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• W2151292912 abstract "The diploid nature of human immunodeficiency virus type 1 (HIV-1) indicates that recombination serves a central function in virus replication and evolution. Previously, while examining the nature of obligatory primer strand transfers during reverse transcription, a high rate of recombination was observed at the ends of the viral genome within the viral long terminal repeats, prompting the following question: does recombination occur at a high rate throughout the genome? To address this question, two vectors based upon different strains of HIV-1 were utilized. The vectors were comprised predominantly of autologous HIV-1 sequence and were approximately the same size as the parental genome. The proviral progeny of heterodimeric virions were analyzed after a single cycle of replication, and the sequence heterogeneity between the two strains allowed direct examination of recombination crossovers. The results obtained indicate that HIV-1 undergoes approximately two to three recombination events per genome per replication cycle. These results imply that both HIV-1 RNAs are typically utilized during reverse transcription and that recombination is an important aspect of HIV-1 replication." @default.
• W2151292912 created "2016-06-24" @default.
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• W2151292912 date "2000-02-01" @default.
• W2151292912 modified "2023-10-18" @default.
• W2151292912 title "High Rate of Recombination throughout the Human Immunodeficiency Virus Type 1 Genome" @default.
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• W2151292912 doi "https://doi.org/10.1128/jvi.74.3.1234-1240.2000" @default.
• W2151292912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/111457" @default.
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