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- W2151297560 abstract "Brook trout oocytes were stripped of their follicular layers and were impaled with both voltage-measuring and current-passing microelectrodes. The membrane resting potential (V m ) was −46 ± 1.2 mV (mean ± SE, n = 50, bath grounded) in control denuded oocytes with an intact germinal vesicle. V m was reduced to −38 ± 1.8 mV (n = 16) in eggs that had ovulated in vivo. Accompanying the potential drop was a marked increase in membrane resistance (R m ) from 26 ± 1.7 in denuded oocytes to 1640 ± 150 kΩ∙cm 2 in ovulated eggs and a reduction in membrane current (I m ) from 2380 ± 260 to 25 ± 2.6 nA∙cm 2 . The increased resistance in ovulated eggs was in part the result of reduced Na + and K + membrane conductances; there was no significant change in Cl − conductance. Treatment of denuded oocytes with 17α-20β-dihydroxyprogesterone produced germinal vesicle dissolution; in denuded oocytes that had undergone germinal vesicle dissolution in vitro, R m increased and I m decreased, comparable at least qualitatively to the changes seen after ovulation in vivo. The results indicate that major electrophysiological changes accompany germinal vesicle dissolution and ovulation in brook trout oocytes." @default.
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- W2151297560 date "1985-08-01" @default.
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- W2151297560 title "Electrophysiology of oocytes during meiotic maturation and after ovulation in brook trout (<i>Salvelinus fontinalis</i>)" @default.
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- W2151297560 doi "https://doi.org/10.1139/z85-283" @default.
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