Matches in SemOpenAlex for { <https://semopenalex.org/work/W2151320130> ?p ?o ?g. }
- W2151320130 endingPage "2274" @default.
- W2151320130 startingPage "2264" @default.
- W2151320130 abstract "Pressure-jump (p-jump)-induced relaxation kinetics was used to explore the energy landscape of protein folding/unfolding of Y115W, a fluorescent variant of ribonuclease A. Pressure-jumps of 40 MPa amplitude (5 ms dead-time) were conducted both to higher (unfolding) and to lower (folding) pressure, in the range from 100 to 500 MPa, between 30 and 50°C. Significant deviations from the expected symmetrical protein relaxation kinetics were observed. Whereas downward p-jumps resulted always in single exponential kinetics, the kinetics induced by upward p-jumps were biphasic in the low pressure range and monophasic at higher pressures. The relative amplitude of the slow phase decreased as a function of both pressure and temperature. At 50°C, only the fast phase remained. These results can be interpreted within the framework of a two-dimensional energy surface containing a pressure- and temperature-dependent barrier between two unfolded states differing in the isomeric state of the Asn-113–Pro-114 bond. Analysis of the activation volume of the fast kinetic phase revealed a temperature-dependent shift of the unfolding transition state to a larger volume. The observed compensation of this effect by glycerol offers an explanation for its protein stabilizing effect." @default.
- W2151320130 created "2016-06-24" @default.
- W2151320130 creator A5006206320 @default.
- W2151320130 creator A5023053290 @default.
- W2151320130 creator A5024319982 @default.
- W2151320130 creator A5033948579 @default.
- W2151320130 creator A5039899869 @default.
- W2151320130 creator A5060598513 @default.
- W2151320130 creator A5077381064 @default.
- W2151320130 date "2006-09-01" @default.
- W2151320130 modified "2023-09-25" @default.
- W2151320130 title "Pressure-Jump-Induced Kinetics Reveals a Hydration Dependent Folding/Unfolding Mechanism of Ribonuclease A" @default.
- W2151320130 cites W1968729198 @default.
- W2151320130 cites W1970586199 @default.
- W2151320130 cites W1974349872 @default.
- W2151320130 cites W1975936699 @default.
- W2151320130 cites W1991723190 @default.
- W2151320130 cites W1992173219 @default.
- W2151320130 cites W1993637257 @default.
- W2151320130 cites W1994872427 @default.
- W2151320130 cites W1999395076 @default.
- W2151320130 cites W1999448894 @default.
- W2151320130 cites W2000208308 @default.
- W2151320130 cites W2004455414 @default.
- W2151320130 cites W2011926767 @default.
- W2151320130 cites W2013679718 @default.
- W2151320130 cites W2014095962 @default.
- W2151320130 cites W2015659955 @default.
- W2151320130 cites W2024477147 @default.
- W2151320130 cites W2024734522 @default.
- W2151320130 cites W2025485410 @default.
- W2151320130 cites W2028719271 @default.
- W2151320130 cites W2029353628 @default.
- W2151320130 cites W2032654131 @default.
- W2151320130 cites W2034095680 @default.
- W2151320130 cites W2038069471 @default.
- W2151320130 cites W2038689972 @default.
- W2151320130 cites W2039622979 @default.
- W2151320130 cites W2044483297 @default.
- W2151320130 cites W2046033652 @default.
- W2151320130 cites W2051446538 @default.
- W2151320130 cites W2052226736 @default.
- W2151320130 cites W2056320307 @default.
- W2151320130 cites W2058708926 @default.
- W2151320130 cites W2065235574 @default.
- W2151320130 cites W2065529346 @default.
- W2151320130 cites W2067323731 @default.
- W2151320130 cites W2073138345 @default.
- W2151320130 cites W2087217304 @default.
- W2151320130 cites W2088254981 @default.
- W2151320130 cites W2089328114 @default.
- W2151320130 cites W2090564161 @default.
- W2151320130 cites W2091205097 @default.
- W2151320130 cites W2092159730 @default.
- W2151320130 cites W2096750810 @default.
- W2151320130 cites W2103598875 @default.
- W2151320130 cites W2108327555 @default.
- W2151320130 cites W2120506843 @default.
- W2151320130 cites W2121506107 @default.
- W2151320130 cites W2131172044 @default.
- W2151320130 cites W2131218736 @default.
- W2151320130 cites W2141119405 @default.
- W2151320130 cites W2152958744 @default.
- W2151320130 cites W2164242608 @default.
- W2151320130 cites W2171755502 @default.
- W2151320130 cites W2171999720 @default.
- W2151320130 cites W2411126243 @default.
- W2151320130 cites W4237226904 @default.
- W2151320130 cites W4240929891 @default.
- W2151320130 doi "https://doi.org/10.1529/biophysj.106.082552" @default.
- W2151320130 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1557576" @default.
- W2151320130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16798802" @default.
- W2151320130 hasPublicationYear "2006" @default.
- W2151320130 type Work @default.
- W2151320130 sameAs 2151320130 @default.
- W2151320130 citedByCount "25" @default.
- W2151320130 countsByYear W21513201302012 @default.
- W2151320130 countsByYear W21513201302014 @default.
- W2151320130 countsByYear W21513201302015 @default.
- W2151320130 countsByYear W21513201302017 @default.
- W2151320130 countsByYear W21513201302018 @default.
- W2151320130 countsByYear W21513201302019 @default.
- W2151320130 countsByYear W21513201302020 @default.
- W2151320130 countsByYear W21513201302022 @default.
- W2151320130 countsByYear W21513201302023 @default.
- W2151320130 crossrefType "journal-article" @default.
- W2151320130 hasAuthorship W2151320130A5006206320 @default.
- W2151320130 hasAuthorship W2151320130A5023053290 @default.
- W2151320130 hasAuthorship W2151320130A5024319982 @default.
- W2151320130 hasAuthorship W2151320130A5033948579 @default.
- W2151320130 hasAuthorship W2151320130A5039899869 @default.
- W2151320130 hasAuthorship W2151320130A5060598513 @default.
- W2151320130 hasAuthorship W2151320130A5077381064 @default.
- W2151320130 hasBestOaLocation W21513201301 @default.
- W2151320130 hasConcept C104317684 @default.
- W2151320130 hasConcept C119599485 @default.
- W2151320130 hasConcept C121332964 @default.
- W2151320130 hasConcept C12554922 @default.