Matches in SemOpenAlex for { <https://semopenalex.org/work/W2151433688> ?p ?o ?g. }
- W2151433688 abstract "Variants of the thermolabile neutral protease (Npr) of B. subtilis (Npr-sub) and the thermostable neutral protease of B. stearothermophilus (Npr-ste) were produced by means of site-directed mutagenesis and the effects of the mutations on thermostability were determined. Mutations were designed to alter the interaction between the middle and C-terminal subdomain of these enzymes. In all Nprs a cluster of hydrophobic contacts centered around residue 315 contributes to this interaction. In thermostable Nprs (like Npr-ste) a 10 residue beta-hairpin, covering the domain interface, makes an additional contribution. The hydrophobic residue at position 315 was replaced by smaller amino acids. In addition, the beta-hairpin was deleted from Npr-ste and inserted into Npr-sub. The changes in thermostability observed after these mutations confirmed the importance of the hydrophobic cluster and of the beta-hairpin for the structural integrity of Nprs. Combined mutants showed that the effects of individual mutations affecting the interaction between the subdomains were not additive. The effects on thermostability decreased as the strength of the subdomain interaction increased. The results show that once the subdomain interface is sufficiently stabilized, additional stabilizing mutations at the same interface do not further increase thermostability. The results are interpreted on the basis of a model for the thermal inactivation of neutral proteases, in which it is assumed that inactivation results from the occurrence of local unfolding processes that render these enzymes susceptible to autolysis." @default.
- W2151433688 created "2016-06-24" @default.
- W2151433688 creator A5000439902 @default.
- W2151433688 creator A5004780974 @default.
- W2151433688 creator A5014259812 @default.
- W2151433688 creator A5015251681 @default.
- W2151433688 creator A5075419050 @default.
- W2151433688 creator A5076571272 @default.
- W2151433688 date "1992-10-01" @default.
- W2151433688 modified "2023-09-27" @default.
- W2151433688 title "Effects of changing the interaction between subdomains on the thermostability ofBacillus neutral proteases" @default.
- W2151433688 cites W121717166 @default.
- W2151433688 cites W1491832097 @default.
- W2151433688 cites W1535820474 @default.
- W2151433688 cites W1541008516 @default.
- W2151433688 cites W1548617948 @default.
- W2151433688 cites W1563029436 @default.
- W2151433688 cites W1572975248 @default.
- W2151433688 cites W1581901221 @default.
- W2151433688 cites W1606011614 @default.
- W2151433688 cites W1806837705 @default.
- W2151433688 cites W1811590717 @default.
- W2151433688 cites W1911747505 @default.
- W2151433688 cites W1950598798 @default.
- W2151433688 cites W1964066260 @default.
- W2151433688 cites W1965758127 @default.
- W2151433688 cites W1971112995 @default.
- W2151433688 cites W1976843839 @default.
- W2151433688 cites W1977794986 @default.
- W2151433688 cites W1992230954 @default.
- W2151433688 cites W1993636042 @default.
- W2151433688 cites W1997064547 @default.
- W2151433688 cites W2006645661 @default.
- W2151433688 cites W2009547515 @default.
- W2151433688 cites W2011259458 @default.
- W2151433688 cites W2016131951 @default.
- W2151433688 cites W2024552188 @default.
- W2151433688 cites W2027027784 @default.
- W2151433688 cites W2029871341 @default.
- W2151433688 cites W2035609022 @default.
- W2151433688 cites W2039026099 @default.
- W2151433688 cites W2044328480 @default.
- W2151433688 cites W2044797251 @default.
- W2151433688 cites W2049115197 @default.
- W2151433688 cites W2049576685 @default.
- W2151433688 cites W2051889716 @default.
- W2151433688 cites W2055451650 @default.
- W2151433688 cites W2055514904 @default.
- W2151433688 cites W2055777918 @default.
- W2151433688 cites W2061033322 @default.
- W2151433688 cites W2069172444 @default.
- W2151433688 cites W2078051636 @default.
- W2151433688 cites W2079414398 @default.
- W2151433688 cites W2080680293 @default.
- W2151433688 cites W2089647605 @default.
- W2151433688 cites W2090418631 @default.
- W2151433688 cites W2100884811 @default.
- W2151433688 cites W2107276905 @default.
- W2151433688 cites W2132717858 @default.
- W2151433688 cites W2138270253 @default.
- W2151433688 cites W2139327615 @default.
- W2151433688 cites W2157821159 @default.
- W2151433688 cites W2324214428 @default.
- W2151433688 cites W2327614281 @default.
- W2151433688 cites W2337507300 @default.
- W2151433688 cites W4213114154 @default.
- W2151433688 doi "https://doi.org/10.1002/prot.340140209" @default.
- W2151433688 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1409570" @default.
- W2151433688 hasPublicationYear "1992" @default.
- W2151433688 type Work @default.
- W2151433688 sameAs 2151433688 @default.
- W2151433688 citedByCount "27" @default.
- W2151433688 countsByYear W21514336882013 @default.
- W2151433688 countsByYear W21514336882014 @default.
- W2151433688 countsByYear W21514336882015 @default.
- W2151433688 countsByYear W21514336882016 @default.
- W2151433688 countsByYear W21514336882019 @default.
- W2151433688 countsByYear W21514336882022 @default.
- W2151433688 crossrefType "journal-article" @default.
- W2151433688 hasAuthorship W2151433688A5000439902 @default.
- W2151433688 hasAuthorship W2151433688A5004780974 @default.
- W2151433688 hasAuthorship W2151433688A5014259812 @default.
- W2151433688 hasAuthorship W2151433688A5015251681 @default.
- W2151433688 hasAuthorship W2151433688A5075419050 @default.
- W2151433688 hasAuthorship W2151433688A5076571272 @default.
- W2151433688 hasConcept C104317684 @default.
- W2151433688 hasConcept C12554922 @default.
- W2151433688 hasConcept C143065580 @default.
- W2151433688 hasConcept C181199279 @default.
- W2151433688 hasConcept C182220744 @default.
- W2151433688 hasConcept C185592680 @default.
- W2151433688 hasConcept C2776714187 @default.
- W2151433688 hasConcept C2779155592 @default.
- W2151433688 hasConcept C2781216036 @default.
- W2151433688 hasConcept C55493867 @default.
- W2151433688 hasConcept C8010536 @default.
- W2151433688 hasConcept C86803240 @default.
- W2151433688 hasConceptScore W2151433688C104317684 @default.
- W2151433688 hasConceptScore W2151433688C12554922 @default.
- W2151433688 hasConceptScore W2151433688C143065580 @default.