FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2151550135> ?p ?o ?g. }

• W2151550135 endingPage "1220" @default.
• W2151550135 startingPage "1212" @default.
• W2151550135 abstract "It is expected that the increasing outbreaks of diabetes mellitus along with introducing alternative of present insulin consumption methods, will result in increasing the demand of such a drug in future. Plant systems have high potential to produce safe, economical, inexpensive and large-scale biopharmaceuticals and potato is one of the most important bioreactors, globally. Most Agrobacterium-mediated transformation procedures referred to in the literatures have proven to be inefficient for the transformation of Agria and Marfona potato cultivars. In this study a novel one- step method was demonstrated for the transformation of the internodal explants of these cultivars. Using this method, human proinsulin gene was expressed in the transgenic potato plants. In this research, the DNA sequence of Immunoglobulin G binding protein taken from Staphylococcus aureus was infused to the human proinsulin gene and this construct was then transferred to the nuclear genome of potato via Agrobacterium-mediated transformation. Assessment of the transgenic plants was carried out in 3 levels of DNA, RNA and protein. Gel analysis of the PCR and RT-PCR products showed a single 500 bp band in the transgenic potato lines. SDS-PAGE analysis also showed a 17-18 kDa weight band, while this band was not shown in the non-transgenic ones. Leaf samples of transgenic potato had a positive reaction in ELISA with the human insulin antibody and dot blot assays were also positive." @default.
• W2151550135 created "2016-06-24" @default.
• W2151550135 creator A5064537794 @default.
• W2151550135 creator A5066877248 @default.
• W2151550135 creator A5083264560 @default.
• W2151550135 creator A5085307600 @default.
• W2151550135 creator A5030395180 @default.
• W2151550135 date "2012-07-01" @default.
• W2151550135 modified "2023-09-27" @default.
• W2151550135 title "Regeneration and Agrobacterium -mediated transformation of three potato cultivars (Solanum tuberosum cv. Desiree, Agria and Marfona) by human proinsulin gene" @default.
• W2151550135 cites W104318735 @default.
• W2151550135 cites W1540585118 @default.
• W2151550135 cites W1968882642 @default.
• W2151550135 cites W1972529907 @default.
• W2151550135 cites W1972934647 @default.
• W2151550135 cites W1978657730 @default.
• W2151550135 cites W197919629 @default.
• W2151550135 cites W1987968780 @default.
• W2151550135 cites W1992686283 @default.
• W2151550135 cites W1996445758 @default.
• W2151550135 cites W1996678163 @default.
• W2151550135 cites W2002974147 @default.
• W2151550135 cites W2030717516 @default.
• W2151550135 cites W2034407708 @default.
• W2151550135 cites W2034885910 @default.
• W2151550135 cites W2041031476 @default.
• W2151550135 cites W2045355832 @default.
• W2151550135 cites W2047844167 @default.
• W2151550135 cites W2050519822 @default.
• W2151550135 cites W2055499790 @default.
• W2151550135 cites W2060777062 @default.
• W2151550135 cites W2070287693 @default.
• W2151550135 cites W2092603929 @default.
• W2151550135 cites W2100837269 @default.
• W2151550135 cites W2110547825 @default.
• W2151550135 cites W2112726433 @default.
• W2151550135 cites W2117510461 @default.
• W2151550135 cites W2118026266 @default.
• W2151550135 cites W2122265866 @default.
• W2151550135 cites W2130259 @default.
• W2151550135 cites W2147402263 @default.
• W2151550135 cites W2162291940 @default.
• W2151550135 cites W2332049991 @default.
• W2151550135 cites W2524683597 @default.
• W2151550135 cites W2187937656 @default.
• W2151550135 cites W2409330278 @default.
• W2151550135 hasPublicationYear "2012" @default.
• W2151550135 type Work @default.
• W2151550135 sameAs 2151550135 @default.
• W2151550135 citedByCount "3" @default.
• W2151550135 countsByYear W21515501352013 @default.
• W2151550135 countsByYear W21515501352016 @default.
• W2151550135 crossrefType "journal-article" @default.
• W2151550135 hasAuthorship W2151550135A5030395180 @default.
• W2151550135 hasAuthorship W2151550135A5064537794 @default.
• W2151550135 hasAuthorship W2151550135A5066877248 @default.
• W2151550135 hasAuthorship W2151550135A5083264560 @default.
• W2151550135 hasAuthorship W2151550135A5085307600 @default.
• W2151550135 hasConcept C102230213 @default.
• W2151550135 hasConcept C104317684 @default.
• W2151550135 hasConcept C122172344 @default.
• W2151550135 hasConcept C150903083 @default.
• W2151550135 hasConcept C197321923 @default.
• W2151550135 hasConcept C204241405 @default.
• W2151550135 hasConcept C2779306644 @default.
• W2151550135 hasConcept C2779656422 @default.
• W2151550135 hasConcept C2993140171 @default.
• W2151550135 hasConcept C54355233 @default.
• W2151550135 hasConcept C59822182 @default.
• W2151550135 hasConcept C86803240 @default.
• W2151550135 hasConcept C90061646 @default.
• W2151550135 hasConceptScore W2151550135C102230213 @default.
• W2151550135 hasConceptScore W2151550135C104317684 @default.
• W2151550135 hasConceptScore W2151550135C122172344 @default.
• W2151550135 hasConceptScore W2151550135C150903083 @default.
• W2151550135 hasConceptScore W2151550135C197321923 @default.
• W2151550135 hasConceptScore W2151550135C204241405 @default.
• W2151550135 hasConceptScore W2151550135C2779306644 @default.
• W2151550135 hasConceptScore W2151550135C2779656422 @default.
• W2151550135 hasConceptScore W2151550135C2993140171 @default.
• W2151550135 hasConceptScore W2151550135C54355233 @default.
• W2151550135 hasConceptScore W2151550135C59822182 @default.
• W2151550135 hasConceptScore W2151550135C86803240 @default.
• W2151550135 hasConceptScore W2151550135C90061646 @default.
• W2151550135 hasIssue "7" @default.
• W2151550135 hasLocation W21515501351 @default.
• W2151550135 hasOpenAccess W2151550135 @default.
• W2151550135 hasPrimaryLocation W21515501351 @default.
• W2151550135 hasRelatedWork W1631295132 @default.
• W2151550135 hasRelatedWork W1970277309 @default.
• W2151550135 hasRelatedWork W1996797906 @default.
• W2151550135 hasRelatedWork W2000117871 @default.
• W2151550135 hasRelatedWork W2024205930 @default.
• W2151550135 hasRelatedWork W2036942177 @default.
• W2151550135 hasRelatedWork W2072987125 @default.
• W2151550135 hasRelatedWork W2086013189 @default.
• W2151550135 hasRelatedWork W2090197027 @default.
• W2151550135 hasRelatedWork W2100629938 @default.

• next