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• W2151819753 abstract "Extended-spectrum β -lactamase (ESBL)-producing Escherichia coli has spread rapidly worldwide and poses a serious threat to human and animal health. This study collected 51 non-replicate E. coli isolates from 14 different chicken farms in Henan Province in China from December 2007 to August 2008. The prevalence of ESBL-producing E. coli , molecular characterization of the ESBL-related bla genes, including bla TEM , bla SHV and bla CTX-M , and the susceptibilities of these bacteria to various antimicrobial agents were determined. Thirty-one of the 51 isolates were positive for an ESBL phenotype and 29 of these isolates carried one or more bla genes. Twenty-two isolates harboured bla TEM genes and 15 isolates carried bla CTX-M genes (one CTX-M-14, three CTX-M-24 and 11 CTX-M-65). One isolate carried bla TEM -57 ; the remaining bla TEM isolates carried bla TEM-1 with one silent nucleotide base variation (T18C). We believe that this is the first study to report TEM-57 in E. coli isolates. All isolates harbouring bla CTX-M-24 and bla CTX-M-14 and five of the bla CTX-M-65 isolates also harboured the bla TEM-1 gene. To our knowledge, this study is the first to describe detection of CTX-M-65-producing E. coli isolated from chickens. None of the isolates contained the bla SHV gene. Conjugation experiments demonstrated that bla CTX-M and bla TEM genes could be transferred to E. coli DH5 α . The results indicate that ESBL frequency has reached an alarming level in chicken isolates in China, with TEM-1 and CTX-M-65 enzymes being the two predominant β -lactamases detected." @default.
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• W2151819753 date "2009-11-01" @default.
• W2151819753 modified "2023-10-01" @default.
• W2151819753 title "Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from chickens in Henan Province, China" @default.
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• W2151819753 doi "https://doi.org/10.1099/jmm.0.012229-0" @default.
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