Matches in SemOpenAlex for { <https://semopenalex.org/work/W2151992425> ?p ?o ?g. }
- W2151992425 endingPage "4198" @default.
- W2151992425 startingPage "4186" @default.
- W2151992425 abstract "Rrp43p is a Saccharomyces cerevisiae exosome subunit involved in pre-rRNA processing which is found both in the nucleus and in the cytoplasm. So far, no function has been assigned to the cytoplasmic fraction of Rrp43p. We have addressed Rrp43p function by analyzing mRNA stability in three rrp43 temperature-sensitive (ts) strains, which carry different ts alleles (rrp43-1, rrp43-2 and rrp43-3), and by analyzing Rrp43p interactions with the remaining exosome subunits. In the ts strains, endogenous mRNAs (ACT1 and PAB1), as well as a heterologous reporter mRNA (CATpG) showed longer half-lives, relative to a control strain carrying wild-type RRP43. The mutants also accumulated a degradation intermediate of the reporter mRNA that is typical of defective mRNA decay. These results allow us to propose that Rrp43p is required for mRNA degradation. Rrp43p interacts with the exosome complex via Rrp46p, as determined by two-hybrid analyses. Interestingly, the rrp43 ts mutant proteins do not interact with Rrp46p, indicating that the ts phenotype may be caused by disruption of the Rrp43p- Rrp46p interaction. The ts strains also showed a pre-rRNA processing defect, which is consistent with previous studies on Rrp43p function." @default.
- W2151992425 created "2016-06-24" @default.
- W2151992425 creator A5056172916 @default.
- W2151992425 date "2002-10-01" @default.
- W2151992425 modified "2023-09-27" @default.
- W2151992425 title "Temperature-sensitive mutants of the exosome subunit Rrp43p show a deficiency in mRNA degradation and no longer interact with the exosome" @default.
- W2151992425 cites W1524542249 @default.
- W2151992425 cites W1585970141 @default.
- W2151992425 cites W164713944 @default.
- W2151992425 cites W1673502203 @default.
- W2151992425 cites W1794942671 @default.
- W2151992425 cites W1903251151 @default.
- W2151992425 cites W1903792504 @default.
- W2151992425 cites W192451759 @default.
- W2151992425 cites W1967390330 @default.
- W2151992425 cites W1971227936 @default.
- W2151992425 cites W1974575813 @default.
- W2151992425 cites W1975593876 @default.
- W2151992425 cites W1975972022 @default.
- W2151992425 cites W1997279341 @default.
- W2151992425 cites W2005982219 @default.
- W2151992425 cites W2008188499 @default.
- W2151992425 cites W2019025253 @default.
- W2151992425 cites W2028513775 @default.
- W2151992425 cites W2028662837 @default.
- W2151992425 cites W2035792284 @default.
- W2151992425 cites W2040010148 @default.
- W2151992425 cites W2042104697 @default.
- W2151992425 cites W2044386270 @default.
- W2151992425 cites W2065317542 @default.
- W2151992425 cites W2072174598 @default.
- W2151992425 cites W2082514511 @default.
- W2151992425 cites W2082658854 @default.
- W2151992425 cites W2084737875 @default.
- W2151992425 cites W2090850551 @default.
- W2151992425 cites W2090951806 @default.
- W2151992425 cites W2096909079 @default.
- W2151992425 cites W2100269820 @default.
- W2151992425 cites W2110544609 @default.
- W2151992425 cites W2111369118 @default.
- W2151992425 cites W2111978918 @default.
- W2151992425 cites W2115761677 @default.
- W2151992425 cites W2120959840 @default.
- W2151992425 cites W2129232563 @default.
- W2151992425 cites W2129648975 @default.
- W2151992425 cites W2132094774 @default.
- W2151992425 cites W2132398035 @default.
- W2151992425 cites W2134910640 @default.
- W2151992425 cites W2146393576 @default.
- W2151992425 cites W2152432732 @default.
- W2151992425 cites W2152656750 @default.
- W2151992425 cites W2156215734 @default.
- W2151992425 cites W2157099873 @default.
- W2151992425 cites W2168545181 @default.
- W2151992425 cites W4294232809 @default.
- W2151992425 doi "https://doi.org/10.1093/nar/gkf545" @default.
- W2151992425 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/140545" @default.
- W2151992425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12364597" @default.
- W2151992425 hasPublicationYear "2002" @default.
- W2151992425 type Work @default.
- W2151992425 sameAs 2151992425 @default.
- W2151992425 citedByCount "30" @default.
- W2151992425 countsByYear W21519924252013 @default.
- W2151992425 countsByYear W21519924252015 @default.
- W2151992425 countsByYear W21519924252016 @default.
- W2151992425 countsByYear W21519924252017 @default.
- W2151992425 countsByYear W21519924252019 @default.
- W2151992425 countsByYear W21519924252020 @default.
- W2151992425 countsByYear W21519924252022 @default.
- W2151992425 crossrefType "journal-article" @default.
- W2151992425 hasAuthorship W2151992425A5056172916 @default.
- W2151992425 hasBestOaLocation W21519924252 @default.
- W2151992425 hasConcept C104292427 @default.
- W2151992425 hasConcept C104317684 @default.
- W2151992425 hasConcept C105580179 @default.
- W2151992425 hasConcept C143065580 @default.
- W2151992425 hasConcept C145059251 @default.
- W2151992425 hasConcept C149364088 @default.
- W2151992425 hasConcept C153911025 @default.
- W2151992425 hasConcept C190062978 @default.
- W2151992425 hasConcept C20518536 @default.
- W2151992425 hasConcept C2777576037 @default.
- W2151992425 hasConcept C2780115692 @default.
- W2151992425 hasConcept C2781261824 @default.
- W2151992425 hasConcept C2991743866 @default.
- W2151992425 hasConcept C54355233 @default.
- W2151992425 hasConcept C75449159 @default.
- W2151992425 hasConcept C86803240 @default.
- W2151992425 hasConcept C95444343 @default.
- W2151992425 hasConceptScore W2151992425C104292427 @default.
- W2151992425 hasConceptScore W2151992425C104317684 @default.
- W2151992425 hasConceptScore W2151992425C105580179 @default.
- W2151992425 hasConceptScore W2151992425C143065580 @default.
- W2151992425 hasConceptScore W2151992425C145059251 @default.
- W2151992425 hasConceptScore W2151992425C149364088 @default.
- W2151992425 hasConceptScore W2151992425C153911025 @default.
- W2151992425 hasConceptScore W2151992425C190062978 @default.
- W2151992425 hasConceptScore W2151992425C20518536 @default.