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• W2152073175 abstract "Second-generation sequencing technology can now be used to sequence an entire human genome in a matter of days and at low cost. Sequence read lengths, initially very short, have rapidly increased since the technology first appeared, and we now are seeing a growing number of efforts to sequence large genomes de novo from these short reads. In this Perspective, we describe the issues associated with short-read assembly, the different types of data produced by second-gen sequencers, and the latest assembly algorithms designed for these data. We also review the genomes that have been assembled recently from short reads and make recommendations for sequencing strategies that will yield a high-quality assembly." @default.
• W2152073175 created "2016-06-24" @default.
• W2152073175 creator A5010384159 @default.
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• W2152073175 date "2010-05-27" @default.
• W2152073175 modified "2023-10-14" @default.
• W2152073175 title "Assembly of large genomes using second-generation sequencing" @default.
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• W2152073175 doi "https://doi.org/10.1101/gr.101360.109" @default.
• W2152073175 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2928494" @default.
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• W2152073175 hasPublicationYear "2010" @default.
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