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- W2152214911 abstract "Objective We aim to isolate and cultivate highly purified brain microvascular endothelial cells, and to establish an in vitro blood-brain barrier(BBB) model.Methods Grey matters from 3-week old rats were homogenized and fractionated by centrifugation method.After digestion, microvessels were incubated in collagen-coated tissue culture flasks.Then the cells were examined under microscope and the expression of factor VⅢ-related antigen was determined.Results Microscopic observation revealed that BMECs were in the fusiform and the cells formed confluence monolayer after 7 days in culture.Most of the cells were found to express factor VⅢ-related antigen.Conclusion Primary BMECs were successfully isolated.Rats BMECs can be used as an in vitro cell model for studying the pathogenesis of microbial meningitis." @default.
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- W2152214911 date "2009-01-01" @default.
- W2152214911 modified "2023-09-24" @default.
- W2152214911 title "Isolation and cultivation of primary rat brain microvascular endothelial cells." @default.
- W2152214911 hasPublicationYear "2009" @default.
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