FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2152302694> ?p ?o ?g. }

• W2152302694 endingPage "288" @default.
• W2152302694 startingPage "279" @default.
• W2152302694 abstract "It is well established that nitric oxide (NO) inhibits vascular smooth muscle cell (VSMC) proliferation by modulating cell cycle proteins. The 26S proteasome is integral to protein degradation and tightly regulates cell cycle proteins. Therefore, we hypothesized that NO directly inhibits the activity of the 26S proteasome. The three enzymatic activities (chymotrypsin-like, trypsin-like and caspase-like) of the 26S proteasome were examined in VSMC. At baseline, caspase-like activity was approximately 3.5-fold greater than chymotrypsin- and trypsin-like activities. The NO donor S-nitroso-N-acetylpenicillamine (SNAP) significantly inhibited all three catalytically active sites in a time- and concentration-dependent manner (P<0.05). Caspase-like activity was inhibited to a greater degree (77.2% P<0.05). cGMP and cAMP analogs and inhibitors had no statistically significant effect on basal or NO-mediated inhibition of proteasome activity. Dithiothreitol, a reducing agent, prevented and reversed the NO-mediated inhibition of the 26S proteasome. Nitroso-cysteine analysis following S-nitrosoglutathione exposure revealed that the 20S catalytic core of the 26S proteasome contains 10 cysteines which were S-nitrosylated by NO. Evaluation of 26S proteasome subunit protein expression revealed differential regulation of the alpha and beta subunits in VSMC following exposure to NO. Finally, immunohistochemical analysis of subunit expression revealed distinct intracellular localization of the 26S proteasomal subunits at baseline and confirmed upregulation of distinct subunits following NO exposure. In conclusion, NO reversibly inhibits the catalytic activity of the 26S proteasome through S-nitrosylation and differentially regulates proteasomal subunit expression. This may be one mechanism by which NO exerts its effects on the cell cycle and inhibits cellular proliferation in the vasculature." @default.
• W2152302694 created "2016-06-24" @default.
• W2152302694 creator A5021095261 @default.
• W2152302694 creator A5050371463 @default.
• W2152302694 creator A5057836141 @default.
• W2152302694 creator A5066978332 @default.
• W2152302694 creator A5086611091 @default.
• W2152302694 date "2009-06-01" @default.
• W2152302694 modified "2023-09-27" @default.
• W2152302694 title "Nitric oxide regulates the 26S proteasome in vascular smooth muscle cells" @default.
• W2152302694 cites W1481997706 @default.
• W2152302694 cites W1549578642 @default.
• W2152302694 cites W1573531091 @default.
• W2152302694 cites W1963494698 @default.
• W2152302694 cites W1973824723 @default.
• W2152302694 cites W1984728211 @default.
• W2152302694 cites W1988803492 @default.
• W2152302694 cites W1991930087 @default.
• W2152302694 cites W1995596496 @default.
• W2152302694 cites W1996588976 @default.
• W2152302694 cites W1997551404 @default.
• W2152302694 cites W2010374269 @default.
• W2152302694 cites W2018970686 @default.
• W2152302694 cites W2021329972 @default.
• W2152302694 cites W2022699060 @default.
• W2152302694 cites W2024660473 @default.
• W2152302694 cites W2031629641 @default.
• W2152302694 cites W2040205891 @default.
• W2152302694 cites W2041075406 @default.
• W2152302694 cites W2046919270 @default.
• W2152302694 cites W2049743082 @default.
• W2152302694 cites W2065156053 @default.
• W2152302694 cites W2073537531 @default.
• W2152302694 cites W2074497710 @default.
• W2152302694 cites W2080346452 @default.
• W2152302694 cites W2086038877 @default.
• W2152302694 cites W2086192909 @default.
• W2152302694 cites W2088848767 @default.
• W2152302694 cites W2093657684 @default.
• W2152302694 cites W2098367634 @default.
• W2152302694 cites W2098750981 @default.
• W2152302694 cites W2111787291 @default.
• W2152302694 cites W2124270277 @default.
• W2152302694 cites W2150123812 @default.
• W2152302694 cites W2170187489 @default.
• W2152302694 cites W2178503716 @default.
• W2152302694 doi "https://doi.org/10.1016/j.niox.2009.02.005" @default.
• W2152302694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19233305" @default.
• W2152302694 hasPublicationYear "2009" @default.
• W2152302694 type Work @default.
• W2152302694 sameAs 2152302694 @default.
• W2152302694 citedByCount "57" @default.
• W2152302694 countsByYear W21523026942012 @default.
• W2152302694 countsByYear W21523026942013 @default.
• W2152302694 countsByYear W21523026942014 @default.
• W2152302694 countsByYear W21523026942015 @default.
• W2152302694 countsByYear W21523026942016 @default.
• W2152302694 countsByYear W21523026942017 @default.
• W2152302694 countsByYear W21523026942018 @default.
• W2152302694 countsByYear W21523026942019 @default.
• W2152302694 countsByYear W21523026942020 @default.
• W2152302694 countsByYear W21523026942021 @default.
• W2152302694 countsByYear W21523026942022 @default.
• W2152302694 crossrefType "journal-article" @default.
• W2152302694 hasAuthorship W2152302694A5021095261 @default.
• W2152302694 hasAuthorship W2152302694A5050371463 @default.
• W2152302694 hasAuthorship W2152302694A5057836141 @default.
• W2152302694 hasAuthorship W2152302694A5066978332 @default.
• W2152302694 hasAuthorship W2152302694A5086611091 @default.
• W2152302694 hasConcept C104292427 @default.
• W2152302694 hasConcept C104317684 @default.
• W2152302694 hasConcept C127561419 @default.
• W2152302694 hasConcept C134018914 @default.
• W2152302694 hasConcept C178790620 @default.
• W2152302694 hasConcept C181199279 @default.
• W2152302694 hasConcept C185592680 @default.
• W2152302694 hasConcept C27740335 @default.
• W2152302694 hasConcept C2776148510 @default.
• W2152302694 hasConcept C2777824588 @default.
• W2152302694 hasConcept C2778367456 @default.
• W2152302694 hasConcept C2779395532 @default.
• W2152302694 hasConcept C2781307694 @default.
• W2152302694 hasConcept C2992686903 @default.
• W2152302694 hasConcept C519581460 @default.
• W2152302694 hasConcept C55493867 @default.
• W2152302694 hasConcept C79879829 @default.
• W2152302694 hasConcept C86803240 @default.
• W2152302694 hasConcept C95444343 @default.
• W2152302694 hasConceptScore W2152302694C104292427 @default.
• W2152302694 hasConceptScore W2152302694C104317684 @default.
• W2152302694 hasConceptScore W2152302694C127561419 @default.
• W2152302694 hasConceptScore W2152302694C134018914 @default.
• W2152302694 hasConceptScore W2152302694C178790620 @default.
• W2152302694 hasConceptScore W2152302694C181199279 @default.
• W2152302694 hasConceptScore W2152302694C185592680 @default.
• W2152302694 hasConceptScore W2152302694C27740335 @default.
• W2152302694 hasConceptScore W2152302694C2776148510 @default.
• W2152302694 hasConceptScore W2152302694C2777824588 @default.

• next