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- W2152326740 abstract "The effects of temperature on the lateral diffusion of fluorescent phospholipids, sterols and proteins in the plasma membranes of maize root cortex protoplasts were monitored using fluorescence photobleaching recovery (FPR). Diffusion parameters were measured in two cultivars of maize having different chilling tolerance. Hydrodynamic theory predicts that the diffusion coefficient, D, should increase with increasing temperature. In the more chilling-tolerant cultivar, however, D for all three probes was nearly insensitive to temperature. In the more chilling-sensitive cultivar, D was also insensitive to temperature over the range from 12 to 21°C, but D for the lipid probes tended to be higher and more variable at lower temperatures. The proportion of probe molecules free to diffuse in the membrane was less than 1 for all probes, and increased significantly with increasing temperature for the protein probe. These results, taken together, support the concept that the plasma membrane contains domains having differing diffusional characteristics. Temperature effects on membrane diffusion are moderated by the existence of these domains to limit significant changes. The observed tendency for higher diffusion coefficients at low temperatures in the chilling-sensitive cultivar may correlate to morphological changes observed with protoplasts of that cultivar at low temperatures." @default.
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- W2152326740 date "1994-12-01" @default.
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- W2152326740 title "Effects of low temperature on lateral diffusion in plasma membranes on maize (Zea mays L.) root cortex protoplasts: relevance to chilling sensitivity" @default.
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- W2152326740 doi "https://doi.org/10.1111/j.1365-3040.1994.tb00530.x" @default.
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