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• W2152363800 abstract "2-thiazoline-4-carboxylic acid (DL-ATC) to L-cysteine. The enzyme-producing conditions were optimized by using response surface methodology to maximize enzyme activity. Glucose, DL-ATC·3H 2O and rotational speed have significant effects on enzyme activity. Their optimal values were 21.7 g/l glucose, 7.3 g/l DL-ATC·3H 2O and 141 rpm rotational speed. Then validation experiments were conducted under optimal conditions. The enzyme activity was up to 2291 U/g, which was close to the predicted maximum enzyme activity (2284 U/g) and 31.4% higher than the initial activity (1743 U/g). A two-step culture was performed with the optimal conditions for cell growth in the first step and enzyme activity in the second step. The results suggested that high carbon/nitrogen (C/N) mass ratio was favorable to enzyme activity and the two-step culture strategy was proposed to be adopted for achieving both high biomass and enzyme production simultaneously." @default.
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• W2152363800 date "2009-04-20" @default.
• W2152363800 modified "2023-09-24" @default.
• W2152363800 title "Optimization of biomass-producing conditions of Micrococcus sp. S-11 for L-cysteine production" @default.
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• W2152363800 doi "https://doi.org/10.5897/ajb2009.000-9235" @default.
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