FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2152420374> ?p ?o ?g. }

• W2152420374 endingPage "541" @default.
• W2152420374 startingPage "535" @default.
• W2152420374 abstract "We report here the design, synthesis and evaluation of a novel photocleavable (PC) biotinylated nucleotide analog, dUTP-PC-Biotin, for DNA polymerase extension reaction to isolate DNA products for mass spectrometry (MS) analysis. This nucleotide analog has a biotin moiety attached to the 5-position of 2'-deoxyribouridine 5'-triphosphate via a photocleavable 2-nitrobenzyl linker. We have demonstrated that dUTP-PC-Biotin can be faithfully incorporated by the DNA polymerase Thermo Sequenase into the growing DNA strand in a DNA polymerase extension reaction and that its incorporation does not hinder the addition of the subsequent nucleotide. Therefore, the DNA extension fragments generated by using the dUTP-PC-Biotin can be efficiently isolated by a streptavidin-coated surface and recovered by near-UV light irradiation at room temperature in mild condition for further analysis without using any chemicals or heat. Single and multiple primer extension reactions were performed using the dUTP-PC-Biotin to generate DNA products for MALDI-TOF MS analysis. Such nucleotide analogs that carry a biotin and a photocleavable linker will allow the isolation and purification of DNA products under mild conditions for MS-based genetic analysis by DNA sequencing or multiplex single nucleotide polymorphism (SNP) detection. Furthermore, these nucleotide analogs should also be useful in isolating DNA-protein complexes under non-denaturing conditions." @default.
• W2152420374 created "2016-06-24" @default.
• W2152420374 creator A5006543775 @default.
• W2152420374 creator A5024297093 @default.
• W2152420374 creator A5083343259 @default.
• W2152420374 creator A5084552494 @default.
• W2152420374 creator A5090220697 @default.
• W2152420374 date "2004-01-21" @default.
• W2152420374 modified "2023-09-29" @default.
• W2152420374 title "Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry" @default.
• W2152420374 cites W1487585044 @default.
• W2152420374 cites W1589411629 @default.
• W2152420374 cites W1966655899 @default.
• W2152420374 cites W1967393314 @default.
• W2152420374 cites W1978349009 @default.
• W2152420374 cites W1987146517 @default.
• W2152420374 cites W1988659744 @default.
• W2152420374 cites W1991394551 @default.
• W2152420374 cites W1996810652 @default.
• W2152420374 cites W2021790416 @default.
• W2152420374 cites W2062723602 @default.
• W2152420374 cites W2082631942 @default.
• W2152420374 cites W2090652275 @default.
• W2152420374 cites W2096884836 @default.
• W2152420374 cites W2104941509 @default.
• W2152420374 cites W2118889049 @default.
• W2152420374 cites W2127230663 @default.
• W2152420374 cites W2129425318 @default.
• W2152420374 cites W2129937879 @default.
• W2152420374 cites W2156922318 @default.
• W2152420374 cites W2168909179 @default.
• W2152420374 doi "https://doi.org/10.1093/nar/gkh198" @default.
• W2152420374 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/373325" @default.
• W2152420374 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14744978" @default.
• W2152420374 hasPublicationYear "2004" @default.
• W2152420374 type Work @default.
• W2152420374 sameAs 2152420374 @default.
• W2152420374 citedByCount "25" @default.
• W2152420374 countsByYear W21524203742012 @default.
• W2152420374 countsByYear W21524203742013 @default.
• W2152420374 countsByYear W21524203742014 @default.
• W2152420374 countsByYear W21524203742015 @default.
• W2152420374 countsByYear W21524203742016 @default.
• W2152420374 countsByYear W21524203742018 @default.
• W2152420374 countsByYear W21524203742022 @default.
• W2152420374 countsByYear W21524203742023 @default.
• W2152420374 crossrefType "journal-article" @default.
• W2152420374 hasAuthorship W2152420374A5006543775 @default.
• W2152420374 hasAuthorship W2152420374A5024297093 @default.
• W2152420374 hasAuthorship W2152420374A5083343259 @default.
• W2152420374 hasAuthorship W2152420374A5084552494 @default.
• W2152420374 hasAuthorship W2152420374A5090220697 @default.
• W2152420374 hasBestOaLocation W21524203741 @default.
• W2152420374 hasConcept C104317684 @default.
• W2152420374 hasConcept C13575015 @default.
• W2152420374 hasConcept C135763542 @default.
• W2152420374 hasConcept C153209595 @default.
• W2152420374 hasConcept C153911025 @default.
• W2152420374 hasConcept C178790620 @default.
• W2152420374 hasConcept C185592680 @default.
• W2152420374 hasConcept C194857396 @default.
• W2152420374 hasConcept C204628709 @default.
• W2152420374 hasConcept C2756471 @default.
• W2152420374 hasConcept C2777563447 @default.
• W2152420374 hasConcept C2778204606 @default.
• W2152420374 hasConcept C3017666073 @default.
• W2152420374 hasConcept C512185932 @default.
• W2152420374 hasConcept C52182516 @default.
• W2152420374 hasConcept C54887055 @default.
• W2152420374 hasConcept C552990157 @default.
• W2152420374 hasConcept C55493867 @default.
• W2152420374 hasConcept C59701697 @default.
• W2152420374 hasConcept C86803240 @default.
• W2152420374 hasConceptScore W2152420374C104317684 @default.
• W2152420374 hasConceptScore W2152420374C13575015 @default.
• W2152420374 hasConceptScore W2152420374C135763542 @default.
• W2152420374 hasConceptScore W2152420374C153209595 @default.
• W2152420374 hasConceptScore W2152420374C153911025 @default.
• W2152420374 hasConceptScore W2152420374C178790620 @default.
• W2152420374 hasConceptScore W2152420374C185592680 @default.
• W2152420374 hasConceptScore W2152420374C194857396 @default.
• W2152420374 hasConceptScore W2152420374C204628709 @default.
• W2152420374 hasConceptScore W2152420374C2756471 @default.
• W2152420374 hasConceptScore W2152420374C2777563447 @default.
• W2152420374 hasConceptScore W2152420374C2778204606 @default.
• W2152420374 hasConceptScore W2152420374C3017666073 @default.
• W2152420374 hasConceptScore W2152420374C512185932 @default.
• W2152420374 hasConceptScore W2152420374C52182516 @default.
• W2152420374 hasConceptScore W2152420374C54887055 @default.
• W2152420374 hasConceptScore W2152420374C552990157 @default.
• W2152420374 hasConceptScore W2152420374C55493867 @default.
• W2152420374 hasConceptScore W2152420374C59701697 @default.
• W2152420374 hasConceptScore W2152420374C86803240 @default.
• W2152420374 hasIssue "2" @default.
• W2152420374 hasLocation W21524203741 @default.
• W2152420374 hasLocation W21524203742 @default.
• W2152420374 hasLocation W21524203743 @default.
• W2152420374 hasLocation W21524203744 @default.

• next