FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2152522747> ?p ?o ?g. }

• W2152522747 endingPage "2156" @default.
• W2152522747 startingPage "2149" @default.
• W2152522747 abstract "Our previous observation that low concentrations of stonefish (Synanceia trachynis) venom elicit spontaneous quantal acetylcholine release from vertebrate motor nerve terminals prompted our present study to purify the quantal transmitter-releasing toxin present in the venom and to characterize the toxin's ability to alter the ultrastructure and immunoreactivity of frog motor nerve terminals. Fractionation of S. trachynis venom by sequential anion exchange fast protein-liquid chromatography (FPLC) and size-exclusion FPLC yielded a highly purified preparation of a membrane-perturbing (haemolytic) protein toxin, named trachynilysin. Trachynilysin (2–20 μg/ml) significantly increased spontaneous quantal acetylcholine release from motor endings, as detected by recording miniature endplate potentials from isolated frog cutaneous pectoris neuromuscular preparations. Ultrastructural analysis of nerve terminals in which quantal acetylcholine release was stimulated to exhaustion by 3 h exposure to trachynilysin revealed swelling of nerve terminals and marked depletion of small clear synaptic vesicles. However, trachynilysin did not induce a parallel depletion of large dense-core vesicles. Large dense-core vesicles contained calcitonin gene-related peptide (CGRP), as revealed by colloidal gold immunostaining, and trachynilysin-treated nerve endings exhibited CGRP-like immunofluorescence similar to that of untreated terminals. Our results indicate that the ability of stonefish venom to elicit spontaneous quantal acetylcholine release from vertebrate motor nerve terminals is a function of trachynilysin, which selectively stimulates the release of small clear synaptic vesicles and impairs the recycling of small clear synaptic vesicles but does not affect the release of large dense-core vesicles. Trachynilysin may be a valuable tool for use in other secretory terminals to discriminate between neurotransmitter and neuropeptide release." @default.
• W2152522747 created "2016-06-24" @default.
• W2152522747 creator A5019459840 @default.
• W2152522747 creator A5033870151 @default.
• W2152522747 creator A5041762507 @default.
• W2152522747 creator A5069599009 @default.
• W2152522747 date "1996-10-01" @default.
• W2152522747 modified "2023-10-11" @default.
• W2152522747 title "Selective Depletion of Clear Synaptic Vesicles and Enhanced Quantal Transmitter Release at Frog Motor Nerve Endings Produced by Trachynilysin, a Protein Toxin Isolated from Stonefish (<i>Synanceia trachynis</i>) Venom" @default.
• W2152522747 cites W1607046039 @default.
• W2152522747 cites W1964028546 @default.
• W2152522747 cites W1967980203 @default.
• W2152522747 cites W1969669037 @default.
• W2152522747 cites W1981509659 @default.
• W2152522747 cites W1983021631 @default.
• W2152522747 cites W1991557342 @default.
• W2152522747 cites W1999230329 @default.
• W2152522747 cites W1999488340 @default.
• W2152522747 cites W2006222347 @default.
• W2152522747 cites W2023004062 @default.
• W2152522747 cites W2023853357 @default.
• W2152522747 cites W2028784666 @default.
• W2152522747 cites W2030030009 @default.
• W2152522747 cites W2031982102 @default.
• W2152522747 cites W2042344199 @default.
• W2152522747 cites W2042921989 @default.
• W2152522747 cites W2047936654 @default.
• W2152522747 cites W2057251498 @default.
• W2152522747 cites W2067563905 @default.
• W2152522747 cites W2075449190 @default.
• W2152522747 cites W2080324921 @default.
• W2152522747 cites W2081506967 @default.
• W2152522747 cites W2087212265 @default.
• W2152522747 cites W2090172076 @default.
• W2152522747 cites W2092125712 @default.
• W2152522747 cites W2093566215 @default.
• W2152522747 cites W2107605597 @default.
• W2152522747 cites W2129090701 @default.
• W2152522747 cites W2139018229 @default.
• W2152522747 cites W2161369269 @default.
• W2152522747 cites W2164517163 @default.
• W2152522747 cites W2211122330 @default.
• W2152522747 cites W36913096 @default.
• W2152522747 cites W4293247451 @default.
• W2152522747 doi "https://doi.org/10.1111/j.1460-9568.1996.tb00736.x" @default.
• W2152522747 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8921306" @default.
• W2152522747 hasPublicationYear "1996" @default.
• W2152522747 type Work @default.
• W2152522747 sameAs 2152522747 @default.
• W2152522747 citedByCount "40" @default.
• W2152522747 countsByYear W21525227472012 @default.
• W2152522747 countsByYear W21525227472013 @default.
• W2152522747 countsByYear W21525227472015 @default.
• W2152522747 countsByYear W21525227472016 @default.
• W2152522747 countsByYear W21525227472017 @default.
• W2152522747 countsByYear W21525227472018 @default.
• W2152522747 countsByYear W21525227472020 @default.
• W2152522747 countsByYear W21525227472021 @default.
• W2152522747 crossrefType "journal-article" @default.
• W2152522747 hasAuthorship W2152522747A5019459840 @default.
• W2152522747 hasAuthorship W2152522747A5033870151 @default.
• W2152522747 hasAuthorship W2152522747A5041762507 @default.
• W2152522747 hasAuthorship W2152522747A5069599009 @default.
• W2152522747 hasConcept C105702510 @default.
• W2152522747 hasConcept C12554922 @default.
• W2152522747 hasConcept C130316041 @default.
• W2152522747 hasConcept C134018914 @default.
• W2152522747 hasConcept C136357698 @default.
• W2152522747 hasConcept C148785051 @default.
• W2152522747 hasConcept C169760540 @default.
• W2152522747 hasConcept C170493617 @default.
• W2152522747 hasConcept C185592680 @default.
• W2152522747 hasConcept C200170125 @default.
• W2152522747 hasConcept C2775910092 @default.
• W2152522747 hasConcept C2775912687 @default.
• W2152522747 hasConcept C2776219046 @default.
• W2152522747 hasConcept C2777240379 @default.
• W2152522747 hasConcept C2779448229 @default.
• W2152522747 hasConcept C2909770972 @default.
• W2152522747 hasConcept C41625074 @default.
• W2152522747 hasConcept C55493867 @default.
• W2152522747 hasConcept C86803240 @default.
• W2152522747 hasConcept C95444343 @default.
• W2152522747 hasConceptScore W2152522747C105702510 @default.
• W2152522747 hasConceptScore W2152522747C12554922 @default.
• W2152522747 hasConceptScore W2152522747C130316041 @default.
• W2152522747 hasConceptScore W2152522747C134018914 @default.
• W2152522747 hasConceptScore W2152522747C136357698 @default.
• W2152522747 hasConceptScore W2152522747C148785051 @default.
• W2152522747 hasConceptScore W2152522747C169760540 @default.
• W2152522747 hasConceptScore W2152522747C170493617 @default.
• W2152522747 hasConceptScore W2152522747C185592680 @default.
• W2152522747 hasConceptScore W2152522747C200170125 @default.
• W2152522747 hasConceptScore W2152522747C2775910092 @default.
• W2152522747 hasConceptScore W2152522747C2775912687 @default.
• W2152522747 hasConceptScore W2152522747C2776219046 @default.
• W2152522747 hasConceptScore W2152522747C2777240379 @default.
• W2152522747 hasConceptScore W2152522747C2779448229 @default.

• next