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• W2152561045 abstract "Vascular endothelial growth factor (VEGF) is involved in angiogenesis, growth, and tumour cell metastasis. VEGF is expressed as alternative splice variants, which exhibit angiogenic and anti-angiogenic properties. We determined the effect of 5-Aza-2′-deoxycytidine (5-dAzaC) DNA methyltransferase (DNMTs) inhibitor on angiogenic and anti-angiogenic VEGF variants expression in immortalized human lung microvascular endothelial cells (HLMEC). Employing reverse transcription, real-time quantitative PCR (RQ-PCR), and Western blot analysis, we determined that 5-dAzaC decreased VEGF121a and VEGF165a angiogenic, and VEGF121b and VEGF165b anti-angiogenic variants expression in HLMEC. However, this DNMTs inhibitor significantly increases expression of VEGF189b anti-angiogenic variant transcript and protein in HLMEC. Our results suggest that the DNMTs activity may have an influence on the expression of angiogenic and anti-angiogenic VEGF variants in human lung microvascular endothelial cells." @default.
• W2152561045 created "2016-06-24" @default.
• W2152561045 creator A5040704094 @default.
• W2152561045 creator A5048107896 @default.
• W2152561045 date "2008-03-01" @default.
• W2152561045 modified "2023-09-27" @default.
• W2152561045 title "5-Aza-2′-deoxycytidine increases the expression of anti-angiogenic vascular endothelial growth factor 189b variant in human lung microvascular endothelial cells" @default.
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• W2152561045 doi "https://doi.org/10.1016/j.biopha.2007.07.015" @default.
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