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• W2152661331 endingPage "5366" @default.
• W2152661331 startingPage "5358" @default.
• W2152661331 abstract "ABSTRACT A novel strategy for identification of Carnobacterium food isolates based on restriction fragment length polymorphism (RFLP) of PCR-amplified 16S-23S ribosomal intergenic spacer regions (ISRs) was developed. PCR amplification from all Carnobacterium strains studied always yielded three ISR amplicons, which were designated the small ISR (S-ISR), the medium ISR (M-ISR), and the large ISR (L-ISR). The lengths of these ISRs varied from one species to another. Carnobacterium divergens NCDO 2763 T and C. mobile DSM 4849 T generated one major S-ISR band (ca. 400 bp) and minor M-ISR and L-ISR bands (ca. 500 and ca. 600 bp, respectively). The ISRs amplified from C. gallinarum NCFB 2766 T and C. piscicola NCDO 2762 T were larger (S-ISR, ca. 600 bp; M-ISR, ca. 700 bp; and L-ISR, ca. 800 bp). The L-ISR contained two tDNAs coding for tRNA Ile and tRNA Ala genes. The M-ISR included one tRNA Ala gene, and the S-ISR did not contain a tDNA gene. The RFLP scheme devised involves estimation of variable PCR product sizes together with Hin fI, Taq I, and Hin dIII restriction analysis. Forty-two isolates yielded four unique band patterns that correctly resolved these isolates into four Carnobacterium species. This method is very suitable for rapid, low-cost identification of a wide variety of Carnobacterium species without sequencing." @default.
• W2152661331 created "2016-06-24" @default.
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• W2152661331 date "2002-11-01" @default.
• W2152661331 modified "2023-10-17" @default.
• W2152661331 title "Differentiation of Closely Related <i>Carnobacterium</i> Food Isolates Based on 16S-23S Ribosomal DNA Intergenic Spacer Region Polymorphism" @default.
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• W2152661331 doi "https://doi.org/10.1128/aem.68.11.5358-5366.2002" @default.
• W2152661331 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/129878" @default.
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