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- W2152768694 abstract "ABSTRACT Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy suppressors of Dd-STATa, of which 4 encoded segments of a known noncoding RNA, dutA . Expression of dutA is specific to the pstA zone, the region where Dd-STATa is activated. In suppressed strains the expression patterns of several putative Dd-STATa target genes become similar to the wild-type strain. In addition, the amount of the tyrosine-phosphorylated form of Dd-STATa is significantly increased in the suppressed strain. These results indicate that partial copies of dutA may act upstream of Dd-STATa to regulate tyrosine phosphorylation by an unknown mechanism." @default.
- W2152768694 created "2016-06-24" @default.
- W2152768694 creator A5003158540 @default.
- W2152768694 creator A5058499479 @default.
- W2152768694 date "2007-06-01" @default.
- W2152768694 modified "2023-10-10" @default.
- W2152768694 title "Evidence that Noncoding RNA <i>dutA</i> Is a Multicopy Suppressor of <i>Dictyostelium discoideum</i> STAT Protein Dd-STATa" @default.
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- W2152768694 doi "https://doi.org/10.1128/ec.00035-07" @default.
- W2152768694 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1951520" @default.
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