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• W2152788788 abstract "AbstractIn order to develope an oral drug delivery system for human Insulin, interactions of human insulin with DEAE-dextran was studied under different conditions. Effects of various inoic strenghts (0.02 M, 0.067 M, 0.01 M and 0.20 M), different temperatures (25C, 37C and 45C) and pH's (6.9, 7.4 and 8) as well as lyophilization and the addtion of urea on the interaction were perfromed. Bound human insulin was separated from the unbound insulin by gel chromatogrphy. Insulin concentratin was determined spectrophotometrically at 276 nm. The amout of fraction bound was calculated form area under the curve of the chromatographic peaks. The experiments showed that ionic strength had no effect on the binding capacity. However, fraction bound was increased with increased temprature. Under a pH range studies, maximum biniding was observed at pH 7.4. The presence of 0.042 M urea seems to increase the binding of insulin to have no effect on insulin intergrity." @default.
• W2152788788 created "2016-06-24" @default.
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• W2152788788 date "1990-01-01" @default.
• W2152788788 modified "2023-09-24" @default.
• W2152788788 title "Interactions of Human Insulin with Deae-Dextran" @default.
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• W2152788788 doi "https://doi.org/10.3109/03639049009114911" @default.
• W2152788788 hasPublicationYear "1990" @default.
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