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• W2152808311 abstract "Introducing a phase shift between diffracted and undiffracted light from a sample is one of the oldest techniques for generating phase contrast in optical microscopy. A similar approach should help improve the contrast and clarity of images collected by scanning X-ray microscopy. Scanning X-ray microscopy focuses radiation to a small spot and probes the sample by raster scanning. It allows information to be obtained from secondary signals such as X-ray fluorescence, which yields an elemental mapping of the sample not available in full-field imaging. The analysis and interpretation from these secondary signals can be considerably enhanced if these data are coupled with structural information from transmission imaging. However, absorption often is negligible and phase contrast has not been easily available. Originally introduced with visible light, Zernike phase contrast1 is a well-established technique in full-field X-ray microscopes for visualization of weakly absorbing samples2,3,4,5,6,7. On the basis of reciprocity, we demonstrate the implementation of Zernike phase contrast in scanning X-ray microscopy, revealing structural detail simultaneously with hard-X-ray trace-element measurements. The method is straightforward to implement without significant influence on the resolution of the fluorescence images and delivers complementary information. We show images of biological specimens that clearly demonstrate the advantage of correlating morphology with elemental information." @default.
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• W2152808311 date "2010-09-12" @default.
• W2152808311 modified "2023-09-27" @default.
• W2152808311 title "Zernike phase contrast in scanning microscopy with X-rays" @default.
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• W2152808311 doi "https://doi.org/10.1038/nphys1765" @default.
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