Matches in SemOpenAlex for { <https://semopenalex.org/work/W2152901832> ?p ?o ?g. }
- W2152901832 endingPage "5283" @default.
- W2152901832 startingPage "5270" @default.
- W2152901832 abstract "ABSTRACT The 5′ nontranslated region (NTR) and the X tail in the 3′ NTR are the least variable parts of the hepatitis C virus (HCV) genome and play an important role in the initiation of RNA synthesis. By using subgenomic replicons of the HCV isolates Con1 (genotype 1) and JFH1 (genotype 2), we characterized the genotype specificities of the replication signals contained in the NTRs. The replacement of the JFH1 5′ NTR and X tail with the corresponding Con1 sequence resulted in a significant decrease in replication efficiency. Exchange of the X tail specifically reduced negative-strand synthesis, whereas substitution of the 5′ NTR impaired the generation of progeny positive strands. In search for the proteins involved in the recognition of genotype-specific initiation signals, we analyzed recombinant nonstructural protein 5B (NS5B) RNA polymerases of both isolates and found some genotype-specific template preference for the 3′ end of positive-strand RNA in vitro. To further address genotype specificity, we constructed a series of intergenotypic replicon chimeras. When combining NS3 to NS5A of Con1 with NS5B of JFH1, we observed more-efficient replication with the genotype 2a X tail, indicating that NS5B recognizes genotype-specific signals in this region. In contrast, a combination of the NS3 helicase with NS5A and NS5B was required to confer genotype specificity to the 5′ NTR. These results present the first genetic evidence for an interaction between helicase, NS5A, and NS5B required for the initiation of RNA synthesis and provide a system for the specific analysis of HCV positive- and negative-strand syntheses." @default.
- W2152901832 created "2016-06-24" @default.
- W2152901832 creator A5015740141 @default.
- W2152901832 creator A5042695802 @default.
- W2152901832 creator A5044057964 @default.
- W2152901832 creator A5045264718 @default.
- W2152901832 creator A5056333353 @default.
- W2152901832 creator A5064924082 @default.
- W2152901832 creator A5072830646 @default.
- W2152901832 date "2007-05-15" @default.
- W2152901832 modified "2023-10-18" @default.
- W2152901832 title "Identification of Determinants Involved in Initiation of Hepatitis C Virus RNA Synthesis by Using Intergenotypic Replicase Chimeras" @default.
- W2152901832 cites W1520082092 @default.
- W2152901832 cites W1531200550 @default.
- W2152901832 cites W1593744374 @default.
- W2152901832 cites W1600974081 @default.
- W2152901832 cites W1735561674 @default.
- W2152901832 cites W1823300406 @default.
- W2152901832 cites W1914838920 @default.
- W2152901832 cites W1914884504 @default.
- W2152901832 cites W1930745477 @default.
- W2152901832 cites W1969742464 @default.
- W2152901832 cites W1972459659 @default.
- W2152901832 cites W1974733477 @default.
- W2152901832 cites W1976844675 @default.
- W2152901832 cites W1978114108 @default.
- W2152901832 cites W1988237823 @default.
- W2152901832 cites W1992212684 @default.
- W2152901832 cites W1995000176 @default.
- W2152901832 cites W1995467090 @default.
- W2152901832 cites W2002468303 @default.
- W2152901832 cites W2003579943 @default.
- W2152901832 cites W2012282947 @default.
- W2152901832 cites W2012808888 @default.
- W2152901832 cites W2019655344 @default.
- W2152901832 cites W2022198259 @default.
- W2152901832 cites W2032451726 @default.
- W2152901832 cites W2032657437 @default.
- W2152901832 cites W2049146226 @default.
- W2152901832 cites W2049867962 @default.
- W2152901832 cites W2053238170 @default.
- W2152901832 cites W2054700330 @default.
- W2152901832 cites W2059942810 @default.
- W2152901832 cites W2064551924 @default.
- W2152901832 cites W2066705292 @default.
- W2152901832 cites W2066931983 @default.
- W2152901832 cites W2068128206 @default.
- W2152901832 cites W2069853592 @default.
- W2152901832 cites W2073385105 @default.
- W2152901832 cites W2076815050 @default.
- W2152901832 cites W2077103006 @default.
- W2152901832 cites W2078887917 @default.
- W2152901832 cites W2089962959 @default.
- W2152901832 cites W2092333889 @default.
- W2152901832 cites W2096803801 @default.
- W2152901832 cites W2098368373 @default.
- W2152901832 cites W2100309515 @default.
- W2152901832 cites W2101164752 @default.
- W2152901832 cites W2106239124 @default.
- W2152901832 cites W2106870343 @default.
- W2152901832 cites W2109191027 @default.
- W2152901832 cites W2110671968 @default.
- W2152901832 cites W2116683273 @default.
- W2152901832 cites W2118123392 @default.
- W2152901832 cites W2118727579 @default.
- W2152901832 cites W2120900689 @default.
- W2152901832 cites W2123893936 @default.
- W2152901832 cites W2127577355 @default.
- W2152901832 cites W2129743974 @default.
- W2152901832 cites W2132034333 @default.
- W2152901832 cites W2134148301 @default.
- W2152901832 cites W2139172730 @default.
- W2152901832 cites W2139624902 @default.
- W2152901832 cites W2140518449 @default.
- W2152901832 cites W2142481964 @default.
- W2152901832 cites W2144881432 @default.
- W2152901832 cites W2146277683 @default.
- W2152901832 cites W2147290736 @default.
- W2152901832 cites W2149765059 @default.
- W2152901832 cites W2151898297 @default.
- W2152901832 cites W2154098206 @default.
- W2152901832 cites W2157712520 @default.
- W2152901832 cites W2161406362 @default.
- W2152901832 cites W2169218501 @default.
- W2152901832 cites W76787895 @default.
- W2152901832 doi "https://doi.org/10.1128/jvi.00032-07" @default.
- W2152901832 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1900214" @default.
- W2152901832 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17344294" @default.
- W2152901832 hasPublicationYear "2007" @default.
- W2152901832 type Work @default.
- W2152901832 sameAs 2152901832 @default.
- W2152901832 citedByCount "96" @default.
- W2152901832 countsByYear W21529018322012 @default.
- W2152901832 countsByYear W21529018322013 @default.
- W2152901832 countsByYear W21529018322014 @default.
- W2152901832 countsByYear W21529018322015 @default.
- W2152901832 countsByYear W21529018322016 @default.
- W2152901832 countsByYear W21529018322018 @default.