Matches in SemOpenAlex for { <https://semopenalex.org/work/W2152964044> ?p ?o ?g. }
- W2152964044 endingPage "678" @default.
- W2152964044 startingPage "664" @default.
- W2152964044 abstract "Chromosome region maintenance 1/exportin1/Exp1/Xpo1 (CRM1) is the major transport receptor for the export of proteins from the nucleus. It binds to nuclear export signals (NESs) that are rich in leucines and other hydrophobic amino acids. The prediction of NESs is difficult because of the extreme recognition flexibility of CRM1. Furthermore, proteins can be exported upon binding to an NES-containing adaptor protein. Here we present an approach for identifying targets of the CRM1-export pathway via quantitative mass spectrometry using stable isotope labeling with amino acids in cell culture. With this approach, we identified >100 proteins from HeLa cells that were depleted from cytosolic fractions and/or enriched in nuclear fractions in the presence of the selective CRM1-inhibitor leptomycin B. Novel and validated substrates are the polyubiquitin-binding protein sequestosome 1, the cancerous inhibitor of protein phosphatase 2A (PP2A), the guanine nucleotide-binding protein-like 3-like protein, the programmed cell death protein 2-like protein, and the cytosolic carboxypeptidase 1 (CCP1). We identified a functional NES in CCP1 that mediates direct binding to the export receptor CRM1. The method will be applicable to other nucleocytoplasmic transport pathways, as well as to the analysis of nucleocytoplasmic shuttling proteins under different growth conditions." @default.
- W2152964044 created "2016-06-24" @default.
- W2152964044 creator A5009859857 @default.
- W2152964044 creator A5015464663 @default.
- W2152964044 creator A5072098512 @default.
- W2152964044 creator A5081435154 @default.
- W2152964044 creator A5089006758 @default.
- W2152964044 date "2013-03-01" @default.
- W2152964044 modified "2023-10-14" @default.
- W2152964044 title "Identification of CRM1-dependent Nuclear Export Cargos Using Quantitative Mass Spectrometry" @default.
- W2152964044 cites W1481612613 @default.
- W2152964044 cites W1509646835 @default.
- W2152964044 cites W1547109494 @default.
- W2152964044 cites W1596002427 @default.
- W2152964044 cites W1627945794 @default.
- W2152964044 cites W1764126564 @default.
- W2152964044 cites W1882963992 @default.
- W2152964044 cites W1902142041 @default.
- W2152964044 cites W1963705823 @default.
- W2152964044 cites W1966448816 @default.
- W2152964044 cites W1968110789 @default.
- W2152964044 cites W1969153003 @default.
- W2152964044 cites W1970315917 @default.
- W2152964044 cites W1971961101 @default.
- W2152964044 cites W1972387348 @default.
- W2152964044 cites W1974041592 @default.
- W2152964044 cites W1974831269 @default.
- W2152964044 cites W1976199526 @default.
- W2152964044 cites W1979153645 @default.
- W2152964044 cites W1981715749 @default.
- W2152964044 cites W1991366673 @default.
- W2152964044 cites W2001271981 @default.
- W2152964044 cites W2003653198 @default.
- W2152964044 cites W2006971960 @default.
- W2152964044 cites W2008922773 @default.
- W2152964044 cites W2009826707 @default.
- W2152964044 cites W2014970708 @default.
- W2152964044 cites W2016153572 @default.
- W2152964044 cites W2017529338 @default.
- W2152964044 cites W2018751664 @default.
- W2152964044 cites W2026635576 @default.
- W2152964044 cites W2026938943 @default.
- W2152964044 cites W2032785100 @default.
- W2152964044 cites W2035244922 @default.
- W2152964044 cites W2039137539 @default.
- W2152964044 cites W2045228408 @default.
- W2152964044 cites W2046721669 @default.
- W2152964044 cites W2051948296 @default.
- W2152964044 cites W2057545775 @default.
- W2152964044 cites W2063394866 @default.
- W2152964044 cites W2065054164 @default.
- W2152964044 cites W2073109412 @default.
- W2152964044 cites W2073252587 @default.
- W2152964044 cites W2078188527 @default.
- W2152964044 cites W2079270430 @default.
- W2152964044 cites W2080752012 @default.
- W2152964044 cites W2083459920 @default.
- W2152964044 cites W2086256514 @default.
- W2152964044 cites W2087794695 @default.
- W2152964044 cites W2089639332 @default.
- W2152964044 cites W2089667459 @default.
- W2152964044 cites W2099729010 @default.
- W2152964044 cites W2105544978 @default.
- W2152964044 cites W2111683138 @default.
- W2152964044 cites W2113504375 @default.
- W2152964044 cites W2114915170 @default.
- W2152964044 cites W2118701021 @default.
- W2152964044 cites W2119483030 @default.
- W2152964044 cites W2128014386 @default.
- W2152964044 cites W2128551987 @default.
- W2152964044 cites W2137574966 @default.
- W2152964044 cites W2140638448 @default.
- W2152964044 cites W2141755885 @default.
- W2152964044 cites W2142762027 @default.
- W2152964044 cites W2143593035 @default.
- W2152964044 cites W2144781426 @default.
- W2152964044 cites W2145798214 @default.
- W2152964044 cites W2150518441 @default.
- W2152964044 cites W2150605329 @default.
- W2152964044 cites W2153504753 @default.
- W2152964044 cites W2153988632 @default.
- W2152964044 cites W2158037819 @default.
- W2152964044 cites W2158217645 @default.
- W2152964044 cites W2159046366 @default.
- W2152964044 cites W2171258458 @default.
- W2152964044 doi "https://doi.org/10.1074/mcp.m112.024877" @default.
- W2152964044 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3591659" @default.
- W2152964044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23242554" @default.
- W2152964044 hasPublicationYear "2013" @default.
- W2152964044 type Work @default.
- W2152964044 sameAs 2152964044 @default.
- W2152964044 citedByCount "86" @default.
- W2152964044 countsByYear W21529640442013 @default.
- W2152964044 countsByYear W21529640442014 @default.
- W2152964044 countsByYear W21529640442015 @default.
- W2152964044 countsByYear W21529640442016 @default.
- W2152964044 countsByYear W21529640442017 @default.
- W2152964044 countsByYear W21529640442018 @default.