FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2152966005> ?p ?o ?g. }

• W2152966005 endingPage "e1003218" @default.
• W2152966005 startingPage "e1003218" @default.
• W2152966005 abstract "The HIV-1 gp120-gp41 complex, which mediates viral fusion and cellular entry, undergoes rapid evolution within its external glycan shield to enable escape from neutralizing antibody (NAb). Understanding how conserved protein determinants retain functionality in the context of such evolution is important for their evaluation and exploitation as potential drug and/or vaccine targets. In this study, we examined how the conserved gp120-gp41 association site, formed by the N- and C-terminal segments of gp120 and the disulfide-bonded region (DSR) of gp41, adapts to glycan changes that are linked to neutralization sensitivity. To this end, a DSR mutant virus (K601D) with defective gp120-association was sequentially passaged in peripheral blood mononuclear cells to select suppressor mutations. We reasoned that the locations of suppressors point to structural elements that are functionally linked to the gp120-gp41 association site. In culture 1, gp120 association and viral replication was restored by loss of the conserved glycan at Asn136 in V1 (T138N mutation) in conjunction with the L494I substitution in C5 within the association site. In culture 2, replication was restored with deletion of the N139INN sequence, which ablates the overlapping Asn141-Asn142-Ser-Ser potential N-linked glycosylation sequons in V1, in conjunction with D601N in the DSR. The 136 and 142 glycan mutations appeared to exert their suppressive effects by altering the dependence of gp120-gp41 interactions on the DSR residues, Leu593, Trp596 and Lys601. The 136 and/or 142 glycan mutations increased the sensitivity of HIV-1 pseudovirions to the glycan-dependent NAbs 2G12 and PG16, and also pooled IgG obtained from HIV-1-infected individuals. Thus adjacent V1 glycans allosterically modulate the distal gp120-gp41 association site. We propose that this represents a mechanism for functional adaptation of the gp120-gp41 association site to an evolving glycan shield in a setting of NAb selection." @default.
• W2152966005 created "2016-06-24" @default.
• W2152966005 creator A5001411115 @default.
• W2152966005 creator A5005856722 @default.
• W2152966005 creator A5045316156 @default.
• W2152966005 creator A5056209665 @default.
• W2152966005 creator A5061230266 @default.
• W2152966005 creator A5076804968 @default.
• W2152966005 creator A5090857215 @default.
• W2152966005 date "2013-04-04" @default.
• W2152966005 modified "2023-09-24" @default.
• W2152966005 title "Allosteric Modulation of the HIV-1 gp120-gp41 Association Site by Adjacent gp120 Variable Region 1 (V1) N-Glycans Linked to Neutralization Sensitivity" @default.
• W2152966005 cites W13108619 @default.
• W2152966005 cites W1505517942 @default.
• W2152966005 cites W1523189068 @default.
• W2152966005 cites W1602599521 @default.
• W2152966005 cites W1628484047 @default.
• W2152966005 cites W1635348995 @default.
• W2152966005 cites W1659719610 @default.
• W2152966005 cites W1664573657 @default.
• W2152966005 cites W1794879226 @default.
• W2152966005 cites W1794989449 @default.
• W2152966005 cites W1842798465 @default.
• W2152966005 cites W1857403217 @default.
• W2152966005 cites W1895363198 @default.
• W2152966005 cites W1953822958 @default.
• W2152966005 cites W1971553870 @default.
• W2152966005 cites W1974092202 @default.
• W2152966005 cites W1981440980 @default.
• W2152966005 cites W1982488181 @default.
• W2152966005 cites W1982887925 @default.
• W2152966005 cites W1984370122 @default.
• W2152966005 cites W1997677784 @default.
• W2152966005 cites W1999053028 @default.
• W2152966005 cites W1999421182 @default.
• W2152966005 cites W2000475809 @default.
• W2152966005 cites W2000884295 @default.
• W2152966005 cites W2005559705 @default.
• W2152966005 cites W2010521915 @default.
• W2152966005 cites W2015263716 @default.
• W2152966005 cites W2015404088 @default.
• W2152966005 cites W2021000829 @default.
• W2152966005 cites W2022125636 @default.
• W2152966005 cites W2023604714 @default.
• W2152966005 cites W2024011553 @default.
• W2152966005 cites W2024588681 @default.
• W2152966005 cites W2024734109 @default.
• W2152966005 cites W2025005245 @default.
• W2152966005 cites W2028234747 @default.
• W2152966005 cites W2029665087 @default.
• W2152966005 cites W2030648131 @default.
• W2152966005 cites W2038233421 @default.
• W2152966005 cites W2038769526 @default.
• W2152966005 cites W2040470885 @default.
• W2152966005 cites W2042476299 @default.
• W2152966005 cites W2046325186 @default.
• W2152966005 cites W2054547688 @default.
• W2152966005 cites W2054805388 @default.
• W2152966005 cites W2055264619 @default.
• W2152966005 cites W2060620553 @default.
• W2152966005 cites W2062204035 @default.
• W2152966005 cites W2062456632 @default.
• W2152966005 cites W2063701397 @default.
• W2152966005 cites W2065283382 @default.
• W2152966005 cites W2068576110 @default.
• W2152966005 cites W2068718867 @default.
• W2152966005 cites W2071513798 @default.
• W2152966005 cites W2072003614 @default.
• W2152966005 cites W2072901265 @default.
• W2152966005 cites W2073044742 @default.
• W2152966005 cites W2075222406 @default.
• W2152966005 cites W2078474080 @default.
• W2152966005 cites W2080089116 @default.
• W2152966005 cites W2083726777 @default.
• W2152966005 cites W2085464725 @default.
• W2152966005 cites W2086572129 @default.
• W2152966005 cites W2092441129 @default.
• W2152966005 cites W2092664889 @default.
• W2152966005 cites W2092772986 @default.
• W2152966005 cites W2093009072 @default.
• W2152966005 cites W2096409198 @default.
• W2152966005 cites W2096702327 @default.
• W2152966005 cites W2098781795 @default.
• W2152966005 cites W2099576234 @default.
• W2152966005 cites W2103803598 @default.
• W2152966005 cites W2109622775 @default.
• W2152966005 cites W2116241527 @default.
• W2152966005 cites W2119193084 @default.
• W2152966005 cites W2120876680 @default.
• W2152966005 cites W2121117011 @default.
• W2152966005 cites W2122617162 @default.
• W2152966005 cites W2125692679 @default.
• W2152966005 cites W2126067457 @default.
• W2152966005 cites W2126817706 @default.
• W2152966005 cites W2126887491 @default.
• W2152966005 cites W2127297918 @default.
• W2152966005 cites W2129274180 @default.
• W2152966005 cites W2130455364 @default.

• next