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- W2153114966 abstract "In this work the migration behavior of DNA in structured microfluidic channels was investigated with sensitive video fluorescence microscopy and molecular dynamic (MD) simulations. We found that MD simulations were in excellent agreement with experimental results. As already demonstrated, shorter L-DNA (48.5 kbp) molecules exhibit a higher mobility than longer T2-DNA (164 kbp) ( 11. By using this size- dependent mobility of DNA in structured microchannels the separation of a mixture of h- and T2-DNA was carried out on a microchip. The standard method for separating DNA by length is electrophoresis which is usually performed in gels or entangled polymer solutions. The need of large amounts of analyte and long separation times makes slab gel electrophoresis cumbersome whereas the incorporation of cross-linked gels or high viscosity polymer solutions in capillaries is not trivial. Furthermore, DNA fragment lengths for separation in capillaries at constant field are limited to 20-30 kbp. In contrast to alternative methods based on artificial gel structures (2) or entropic traps (3) our approach focuses on a gel-free method based on topographical structuring of polydimethylsiloxan (PDMS) microfluidic channels (l) produced through rapid prototyping. The migration of h- and T2-DNA stained with the bisintercalator YOYO-1 was observed with sensitive fluorescence video microscopy in 1.5, 3 and 5 urn structured microchannels. MD simulations were carried out for parameters comparable to 5 pm channels and large DNA molecules, e.g. h- and T2-DNA. As demonstrated by Duong et al. (l) the resulting mobility in a 3 and 1.5 urn channel was dependent on the DNA size. Due to these results the separation of a DNA sample in free solution was performed in the structured microchannels." @default.
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- W2153114966 date "2003-01-01" @default.
- W2153114966 modified "2023-09-22" @default.
- W2153114966 title "GEL-FREE ELECTROPHORESIS OF h- AND T2-DNA IN STRUCTURED PDMS MICROFLUIDIC DEVICES" @default.
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