FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153203308> ?p ?o ?g. }

• W2153203308 endingPage "354" @default.
• W2153203308 startingPage "344" @default.
• W2153203308 abstract "A complete, integrated process for the production of an innovative formulation of penicillin G acylase from Providencia rettgeri(rPACP.rett)of industrial applicability is reported. In order to improve the yield of rPAC, the clone LN5.5, carrying four copies of pac gene integrated into the genome of Pichia pastoris, was constructed. The proteinase activity of the recombinant strain was reduced by knockout of the PEP4 gene encoding for proteinase A, resulting in an increased rPACP.rett activity of approximately 40% (3.8 U/mL vs. 2.7U/mL produced by LN5.5 in flask). A high cell density fermentation process was established with a 5-day methanol induction phase and a final PAC activity of up to 27 U/mL. A single step rPACP.rett purification was also developed with an enzyme activity yield of approximately 95%. The novel features of the rPAC P.rett expressed in P.pastoris were fully exploited and emphasized through the covalent immobilization of rPACP.rett. The enzyme wasimmobilized on a series of structurally correlated methacrylic polymers, specifically designed and produced for optimizing rPACP.rett performances in both hydrolytic and synthetic processes. Polymers presenting aminic functionalities were the most efficient, leading to formulations with higher activity and stability (half time stability >3 years and specific activity ranging from 237 to 477 U/g dry based on benzylpenicillin hydrolysis). The efficiency of the immobilized rPACP.rett was finally evaluated by studying the kinetically controlled synthesis of β-lactam antibiotics (cephalexin) and estimating the synthesis/hydrolysis ratio (S/H), which is a crucial parameter for the feasibility of the process. © 2005 Wiley Periodicals, Inc." @default.
• W2153203308 created "2016-06-24" @default.
• W2153203308 creator A5005433430 @default.
• W2153203308 creator A5005792370 @default.
• W2153203308 creator A5022136580 @default.
• W2153203308 creator A5036035626 @default.
• W2153203308 creator A5043499966 @default.
• W2153203308 creator A5046795553 @default.
• W2153203308 creator A5049925360 @default.
• W2153203308 creator A5061464857 @default.
• W2153203308 creator A5086341808 @default.
• W2153203308 date "2006-01-01" @default.
• W2153203308 modified "2023-09-23" @default.
• W2153203308 title "High-level production and covalent immobilization ofProvidencia rettgeri penicillin G acylase (PAC) from recombinantPichia pastoris for the development of a novel and stable biocatalyst of industrial applicability" @default.
• W2153203308 cites W1520847024 @default.
• W2153203308 cites W156929183 @default.
• W2153203308 cites W1961284860 @default.
• W2153203308 cites W1966164969 @default.
• W2153203308 cites W1971401041 @default.
• W2153203308 cites W1977733114 @default.
• W2153203308 cites W1980828647 @default.
• W2153203308 cites W1985406649 @default.
• W2153203308 cites W1987017680 @default.
• W2153203308 cites W1988270240 @default.
• W2153203308 cites W1991326742 @default.
• W2153203308 cites W1999518578 @default.
• W2153203308 cites W2001778365 @default.
• W2153203308 cites W2003915119 @default.
• W2153203308 cites W2010116385 @default.
• W2153203308 cites W2019157316 @default.
• W2153203308 cites W2019230708 @default.
• W2153203308 cites W2023853843 @default.
• W2153203308 cites W2028875171 @default.
• W2153203308 cites W2033137089 @default.
• W2153203308 cites W2035240741 @default.
• W2153203308 cites W2035744556 @default.
• W2153203308 cites W2039201478 @default.
• W2153203308 cites W2043517011 @default.
• W2153203308 cites W2044430464 @default.
• W2153203308 cites W2051403769 @default.
• W2153203308 cites W2053846879 @default.
• W2153203308 cites W2058919089 @default.
• W2153203308 cites W2059522016 @default.
• W2153203308 cites W2060094660 @default.
• W2153203308 cites W2064762423 @default.
• W2153203308 cites W2068605760 @default.
• W2153203308 cites W2070744344 @default.
• W2153203308 cites W2071076638 @default.
• W2153203308 cites W2071951841 @default.
• W2153203308 cites W2076222724 @default.
• W2153203308 cites W2076871649 @default.
• W2153203308 cites W2079626310 @default.
• W2153203308 cites W2079975830 @default.
• W2153203308 cites W2082037029 @default.
• W2153203308 cites W2083174956 @default.
• W2153203308 cites W2085101776 @default.
• W2153203308 cites W2088749908 @default.
• W2153203308 cites W2088779150 @default.
• W2153203308 cites W2090053392 @default.
• W2153203308 cites W2090369121 @default.
• W2153203308 cites W2094647226 @default.
• W2153203308 cites W2100024090 @default.
• W2153203308 cites W2107248551 @default.
• W2153203308 cites W2111817213 @default.
• W2153203308 cites W2118125226 @default.
• W2153203308 cites W2131846154 @default.
• W2153203308 cites W2150256848 @default.
• W2153203308 cites W21949354 @default.
• W2153203308 cites W2330852867 @default.
• W2153203308 cites W2416484454 @default.
• W2153203308 cites W4239882984 @default.
• W2153203308 cites W4256467154 @default.
• W2153203308 cites W4293247451 @default.
• W2153203308 doi "https://doi.org/10.1002/bit.20728" @default.
• W2153203308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16259000" @default.
• W2153203308 hasPublicationYear "2006" @default.
• W2153203308 type Work @default.
• W2153203308 sameAs 2153203308 @default.
• W2153203308 citedByCount "15" @default.
• W2153203308 countsByYear W21532033082014 @default.
• W2153203308 countsByYear W21532033082015 @default.
• W2153203308 countsByYear W21532033082017 @default.
• W2153203308 countsByYear W21532033082020 @default.
• W2153203308 crossrefType "journal-article" @default.
• W2153203308 hasAuthorship W2153203308A5005433430 @default.
• W2153203308 hasAuthorship W2153203308A5005792370 @default.
• W2153203308 hasAuthorship W2153203308A5022136580 @default.
• W2153203308 hasAuthorship W2153203308A5036035626 @default.
• W2153203308 hasAuthorship W2153203308A5043499966 @default.
• W2153203308 hasAuthorship W2153203308A5046795553 @default.
• W2153203308 hasAuthorship W2153203308A5049925360 @default.
• W2153203308 hasAuthorship W2153203308A5061464857 @default.
• W2153203308 hasAuthorship W2153203308A5086341808 @default.
• W2153203308 hasConcept C100544194 @default.
• W2153203308 hasConcept C104317684 @default.
• W2153203308 hasConcept C178790620 @default.
• W2153203308 hasConcept C180577832 @default.
• W2153203308 hasConcept C181199279 @default.

• next