FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153222771> ?p ?o ?g. }

• W2153222771 endingPage "864" @default.
• W2153222771 startingPage "854" @default.
• W2153222771 abstract "Endosomal escape and nuclear localization are two barriers to gene delivery that need to be addressed in the design of new nonviral gene delivery vehicles. We have previously synthesized low-toxicity polyethylene glycol (PEG)-based vehicles with endosomal escape functionalities, but it was determined that the transfection efficiency of PEG-based vehicles that escaped the endosome was still limited by poor nuclear localization. Two different nuclear localization signal (NLS) peptides, SV40 and TAT, were coupled to PEG-based vehicles with DNA-binding peptides (DBPs) to determine the effect of NLS peptides on the transfection efficiency of PEG-based gene delivery vehicles. Coupling one SV40 peptide, a classical NLS, or two TAT peptides, a nonclassical NLS, to PEG–DBP vehicles increased the transfection efficiency of PEG–DBP/DNA particles 15-fold and resulted in similar efficiency to that of a common cationic polymer vehicle, polyethylenimine (PEI). The transfection efficiency of both types of PEG–DBP–NLS particles was further increased 7-fold in the presence of chloroquine, suggesting that the transfection efficiency of PEG–DBP–NLS particles is limited by their ability to escape the endosome. To develop particles that could escape the endosome and target the nucleus, a mixture of PEG–DBP–NLS vehicles and PEG-based vehicles with DBPs and endosomal escape peptides were complexed with plasmid DNA to form multifunctional particles that had a transfection efficiency 2–3 times higher than that of PEI. Additionally, the PEG-based vehicles were less toxic and more resistant to nonspecific protein adsorption than PEI, making them an attractive alternative for nonviral gene delivery." @default.
• W2153222771 created "2016-06-24" @default.
• W2153222771 creator A5000647986 @default.
• W2153222771 creator A5008091309 @default.
• W2153222771 creator A5088465962 @default.
• W2153222771 date "2009-03-01" @default.
• W2153222771 modified "2023-10-17" @default.
• W2153222771 title "Characterization of a multifunctional PEG-based gene delivery system containing nuclear localization signals and endosomal escape peptides" @default.
• W2153222771 cites W1597997684 @default.
• W2153222771 cites W1965665097 @default.
• W2153222771 cites W1975366433 @default.
• W2153222771 cites W1976067433 @default.
• W2153222771 cites W1976811878 @default.
• W2153222771 cites W1977502221 @default.
• W2153222771 cites W1983844422 @default.
• W2153222771 cites W1986045651 @default.
• W2153222771 cites W1994294376 @default.
• W2153222771 cites W1995574753 @default.
• W2153222771 cites W2001255025 @default.
• W2153222771 cites W2001582257 @default.
• W2153222771 cites W2003904826 @default.
• W2153222771 cites W2008568654 @default.
• W2153222771 cites W2013603367 @default.
• W2153222771 cites W2019144805 @default.
• W2153222771 cites W2023532191 @default.
• W2153222771 cites W2024362853 @default.
• W2153222771 cites W2026065678 @default.
• W2153222771 cites W2031123577 @default.
• W2153222771 cites W2031332074 @default.
• W2153222771 cites W2036354853 @default.
• W2153222771 cites W2039669270 @default.
• W2153222771 cites W2041607789 @default.
• W2153222771 cites W2042143995 @default.
• W2153222771 cites W2043069157 @default.
• W2153222771 cites W2045340668 @default.
• W2153222771 cites W2046160343 @default.
• W2153222771 cites W2048432868 @default.
• W2153222771 cites W2051712296 @default.
• W2153222771 cites W2056842925 @default.
• W2153222771 cites W2071286357 @default.
• W2153222771 cites W2079335891 @default.
• W2153222771 cites W2083597639 @default.
• W2153222771 cites W2084193227 @default.
• W2153222771 cites W2086274688 @default.
• W2153222771 cites W2093353723 @default.
• W2153222771 cites W2093881907 @default.
• W2153222771 cites W2094101506 @default.
• W2153222771 cites W2102342643 @default.
• W2153222771 cites W2105668301 @default.
• W2153222771 cites W2113972541 @default.
• W2153222771 cites W2118878458 @default.
• W2153222771 cites W2130805719 @default.
• W2153222771 cites W2138834926 @default.
• W2153222771 cites W2151142281 @default.
• W2153222771 cites W2165306451 @default.
• W2153222771 cites W2325056821 @default.
• W2153222771 doi "https://doi.org/10.1016/j.actbio.2008.09.009" @default.
• W2153222771 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18926782" @default.
• W2153222771 hasPublicationYear "2009" @default.
• W2153222771 type Work @default.
• W2153222771 sameAs 2153222771 @default.
• W2153222771 citedByCount "60" @default.
• W2153222771 countsByYear W21532227712012 @default.
• W2153222771 countsByYear W21532227712013 @default.
• W2153222771 countsByYear W21532227712014 @default.
• W2153222771 countsByYear W21532227712016 @default.
• W2153222771 countsByYear W21532227712017 @default.
• W2153222771 countsByYear W21532227712018 @default.
• W2153222771 countsByYear W21532227712020 @default.
• W2153222771 countsByYear W21532227712021 @default.
• W2153222771 countsByYear W21532227712022 @default.
• W2153222771 countsByYear W21532227712023 @default.
• W2153222771 crossrefType "journal-article" @default.
• W2153222771 hasAuthorship W2153222771A5000647986 @default.
• W2153222771 hasAuthorship W2153222771A5008091309 @default.
• W2153222771 hasAuthorship W2153222771A5088465962 @default.
• W2153222771 hasConcept C10138342 @default.
• W2153222771 hasConcept C102747710 @default.
• W2153222771 hasConcept C104317684 @default.
• W2153222771 hasConcept C12554922 @default.
• W2153222771 hasConcept C135983454 @default.
• W2153222771 hasConcept C1491633281 @default.
• W2153222771 hasConcept C153911025 @default.
• W2153222771 hasConcept C162324750 @default.
• W2153222771 hasConcept C185592680 @default.
• W2153222771 hasConcept C186435350 @default.
• W2153222771 hasConcept C192562407 @default.
• W2153222771 hasConcept C2775925850 @default.
• W2153222771 hasConcept C2776964284 @default.
• W2153222771 hasConcept C2780114586 @default.
• W2153222771 hasConcept C2780723820 @default.
• W2153222771 hasConcept C49805395 @default.
• W2153222771 hasConcept C54009773 @default.
• W2153222771 hasConcept C54400483 @default.
• W2153222771 hasConcept C55493867 @default.
• W2153222771 hasConcept C59157529 @default.
• W2153222771 hasConcept C86803240 @default.
• W2153222771 hasConcept C95444343 @default.

• next