Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153242244> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2153242244 endingPage "33" @default.
- W2153242244 startingPage "27" @default.
- W2153242244 abstract "Abstract Molecular species identification is becoming more wide-spread in diagnostics and ecological studies, particularly with regard to insects for which morphological identification is difficult or time-consuming. In this study, we describe the development and application of a single-step multiplex PCR for the identification of three mealybug species (Hemiptera: Pseudococcidae) associated with grapevine in South Africa: Planococcus ficus (vine mealybug), Planococcus citri (citrus mealybug) and Pseudococcus longispinus (longtailed mealybug). Mealybugs are pests on many commercial crops, including grapevine, in which they transmit viral diseases. Morphological identification of mealybug species is usually time-consuming, requires a high level of taxonomic expertise and usually only adult females can be identified. The single-step multiplex PCR developed here, based on the mitochondrial cytochrome c oxidase subunit 1 (CO I) gene, is rapid, reliable, sensitive, accurate and simple. The entire identification protocol (including DNA extraction, PCR and electrophoresis) can be completed in approximately four hours. Successful DNA extraction from laboratory and unparasitized field-collected individuals stored in absolute ethanol was 97%. Specimens from which DNA could be extracted were always correctly identified (100% accuracy). The technique developed is simple enough to be implemented in any molecular laboratory. The principles described here can be extended to any organism for which rapid, reliable identification is needed." @default.
- W2153242244 created "2016-06-24" @default.
- W2153242244 creator A5004075940 @default.
- W2153242244 creator A5024547776 @default.
- W2153242244 creator A5075326057 @default.
- W2153242244 date "2007-12-13" @default.
- W2153242244 modified "2023-09-23" @default.
- W2153242244 title "A multiplex PCR assay for the simultaneous identification of three mealybug species (Hemiptera: Pseudococcidae)" @default.
- W2153242244 cites W1538029815 @default.
- W2153242244 cites W1926938475 @default.
- W2153242244 cites W1971707621 @default.
- W2153242244 cites W1978336924 @default.
- W2153242244 cites W1992848977 @default.
- W2153242244 cites W2000249279 @default.
- W2153242244 cites W2042208865 @default.
- W2153242244 cites W2064067799 @default.
- W2153242244 cites W2085525886 @default.
- W2153242244 cites W2097382368 @default.
- W2153242244 cites W2099871331 @default.
- W2153242244 cites W2099901798 @default.
- W2153242244 cites W2106882534 @default.
- W2153242244 cites W2107378193 @default.
- W2153242244 cites W2126107269 @default.
- W2153242244 cites W2132339567 @default.
- W2153242244 cites W2136208626 @default.
- W2153242244 cites W2137314121 @default.
- W2153242244 cites W2141718064 @default.
- W2153242244 cites W2144817874 @default.
- W2153242244 cites W2151197437 @default.
- W2153242244 cites W2327674792 @default.
- W2153242244 doi "https://doi.org/10.1017/s000748530700538x" @default.
- W2153242244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18076775" @default.
- W2153242244 hasPublicationYear "2007" @default.
- W2153242244 type Work @default.
- W2153242244 sameAs 2153242244 @default.
- W2153242244 citedByCount "54" @default.
- W2153242244 countsByYear W21532422442012 @default.
- W2153242244 countsByYear W21532422442013 @default.
- W2153242244 countsByYear W21532422442014 @default.
- W2153242244 countsByYear W21532422442015 @default.
- W2153242244 countsByYear W21532422442016 @default.
- W2153242244 countsByYear W21532422442017 @default.
- W2153242244 countsByYear W21532422442018 @default.
- W2153242244 countsByYear W21532422442019 @default.
- W2153242244 countsByYear W21532422442020 @default.
- W2153242244 countsByYear W21532422442021 @default.
- W2153242244 countsByYear W21532422442022 @default.
- W2153242244 crossrefType "journal-article" @default.
- W2153242244 hasAuthorship W2153242244A5004075940 @default.
- W2153242244 hasAuthorship W2153242244A5024547776 @default.
- W2153242244 hasAuthorship W2153242244A5075326057 @default.
- W2153242244 hasConcept C104317684 @default.
- W2153242244 hasConcept C116834253 @default.
- W2153242244 hasConcept C22508944 @default.
- W2153242244 hasConcept C24586158 @default.
- W2153242244 hasConcept C2778908745 @default.
- W2153242244 hasConcept C2779905978 @default.
- W2153242244 hasConcept C2781188995 @default.
- W2153242244 hasConcept C49105822 @default.
- W2153242244 hasConcept C54355233 @default.
- W2153242244 hasConcept C59822182 @default.
- W2153242244 hasConcept C78063203 @default.
- W2153242244 hasConcept C86803240 @default.
- W2153242244 hasConcept C90583042 @default.
- W2153242244 hasConceptScore W2153242244C104317684 @default.
- W2153242244 hasConceptScore W2153242244C116834253 @default.
- W2153242244 hasConceptScore W2153242244C22508944 @default.
- W2153242244 hasConceptScore W2153242244C24586158 @default.
- W2153242244 hasConceptScore W2153242244C2778908745 @default.
- W2153242244 hasConceptScore W2153242244C2779905978 @default.
- W2153242244 hasConceptScore W2153242244C2781188995 @default.
- W2153242244 hasConceptScore W2153242244C49105822 @default.
- W2153242244 hasConceptScore W2153242244C54355233 @default.
- W2153242244 hasConceptScore W2153242244C59822182 @default.
- W2153242244 hasConceptScore W2153242244C78063203 @default.
- W2153242244 hasConceptScore W2153242244C86803240 @default.
- W2153242244 hasConceptScore W2153242244C90583042 @default.
- W2153242244 hasIssue "1" @default.
- W2153242244 hasLocation W21532422441 @default.
- W2153242244 hasLocation W21532422442 @default.
- W2153242244 hasOpenAccess W2153242244 @default.
- W2153242244 hasPrimaryLocation W21532422441 @default.
- W2153242244 hasRelatedWork W1979544728 @default.
- W2153242244 hasRelatedWork W2094290210 @default.
- W2153242244 hasRelatedWork W2153242244 @default.
- W2153242244 hasRelatedWork W2169443269 @default.
- W2153242244 hasRelatedWork W2171349802 @default.
- W2153242244 hasRelatedWork W2393788122 @default.
- W2153242244 hasRelatedWork W2608881761 @default.
- W2153242244 hasRelatedWork W2714531542 @default.
- W2153242244 hasRelatedWork W2760189234 @default.
- W2153242244 hasRelatedWork W2883539575 @default.
- W2153242244 hasVolume "98" @default.
- W2153242244 isParatext "false" @default.
- W2153242244 isRetracted "false" @default.
- W2153242244 magId "2153242244" @default.
- W2153242244 workType "article" @default.