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- W2153245100 abstract "Cysts from nematode-infested plots on eight potato farms in the Sandveld and Ceres regions of South Africa were analysed by means of rDNA-RFLP. The size of the PCR amplification products for all populations was typical for the genus Globodera . Restriction digestion of the amplified products with Msp I and Hinf I confirmed Globodera rostochiensistol/Ig be present in both the Sandveld and Ceres regions and Globodera pallidatol/Ig be absent from South African potato cyst nematode populations. However, several populations from the Sandveld region showed no recognition of the Hinf I restriction site and no digestion took place, indicating them to be a distinct Globodera species. Sequencing of the ITS1 region confirmed the presence of G. rostochiensisl/Ig and an unknown Globodera species, and the absence of G. pallidal/Ig in South Africa. Species-specific primers for the identification of G. rostochiensisl/Ig and G. pallidal/Ig were optimised." @default.
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- W2153245100 date "2006-01-01" @default.
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- W2153245100 title "Differentiation of South African potato cyst nematodes (PCN) by analysis of the rDNA internal transcribed spacer region" @default.
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