FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153249512> ?p ?o ?g. }

• W2153249512 abstract "Cedar Bog Nature Preserve, located near Urbana, OH, encompasses several wetland types including an alkaline fen. In this fen, groundwater emerges in quicksand-like discharge zones consisting of porous Ca/Mg carbonates mixed with organic detritus. This study evaluates seasonal changes in the heterotrophic sediment microbial communities, their response to nutrient amendment, and in the groundwater chemistry from a fen discharge zone at Cedar Bog. The hypothesis that the microbial community in this fen upwelling is nutrient limited throughout the year, particularly by C and P, was tested. The activity of the heterotrophic bacterial community in the sediment compartment was measured. A series of single factor experiments were conducted to study organic and inorganic nutrient regulation of these communities and to determine what nutrients, if any, were limiting. Activities were based on H-thymidine incorporation into DNA by control and nutrient amended sediment slurries and verified with C-leucine incorporation into protein. Bacterial cell abundance was determined using Acridine Orange direct counts. Samples amended with carbon showed significant increases in activity in three of four seasons tested. Bog extract also stimulated activity above that of the control for the winter microcosm. The site bacterial activity also appears to be limited by inorganic nitrogen and possibly phosphorus in summer. OHIO J SCI 104 (3):43–50, 2004 Manuscript received 16 October 2002 and in revised form 9 June 2003 (#02-21). Corresponding Author: Governors State University, University Park, IL 60466-0975 USA. Ph: 708/534-4921; Fax: 708/534-1641; Email: t-gsell@govst.edu Present Address: Department of Biology, Wartburg College, Waverly, IA 50677. INTRODUCTION It has long been recognized that bacteria play an important role in nutrient cycling and secondary production in aquatic systems (Bell and others 1983; Elser and others 1995; Fuhrman and Azam 1980; Riemann and Bell 1990; Taylor and Joint 1990). While extensive research has been conducted on the microbiology and nutrient response of bacteria found in aquatic environments (Chrzanowski and others 1995; Ducklow and others 1985; Heinanen and Kuparinen 1992; Hessen 1992; Kuparinen and Heinanen 1993; Meyer and Tate 1983) and in groundwaters (White and others 1982), little is known about the heterotrophic bacterial communities in alkaline fens (Groffman and others 1996: Kang and others 1998). This research was conducted to see if the carbon, nitrogen, and phosphorus levels in this wetland impact the microbial numbers, growth rates, and physiological status in an Ohio fen. In the Cedar Bog Nature Preserve, alkaline fen upwellings occur where groundwater emerges in discharge zones consisting of porous Ca/Mg carbonates mixed with organic detritus. Since these upwellings are the major source of water and nutrients for the wetland, bacterial growth in the upwellings may represent a significant portion of the system’s bacterial secondary production. It is not clear to what extent nutrients limit bacterial activity in these groundwater-saturated sediments. Laboratory microcosms were used to test the hypothesis that nutrients limit the activity and possibly regulate the physiological status of the bacteria in the fen sediments. This was accomplished using bacterial growth rates for control and nutrient amended sediment slurries and total bacterial cell abundance. Specifically, it was hypothesized that dissolved organic matter (DOM) and phosphate limits the growth of bacteria in the alkaline fen discharge zone. Nitrogen levels were known to be relatively high due to local agricultural seepage of anhydrous ammonia and its microbially converted products, and were therefore hypothesized to not be limiting to the sediment microorganisms. It was also recognized that nitrification would not be carbon limited and therefore components of the microbial communities could be impacted by nitrogen shortages, but not by organic carbon levels. The activity of bacterial communities from various ecosystems has been determined using radiotracer incorporation techniques. H-thymidine incorporation is ubiquitous for heterotrophic bacterial DNA and not taken up by cyanobacteria, eucaryotic microalgae, autotrophic bacteria, or fungi (Baath and Johansson 1990; Thorn and Ventullo 1988). H-thymidine labeling of DNA has been used extensively to measure bacterial activity, however there has been concern over a lack of specificity of incorporation in addition to extraction variability observed in freshwater (Chin-Leo and Kirchman 1988; Robarts and others 1986) and sediment (Brittain and Karl 1990; Ducklow and others 1985; Moriarty and Pollard 1981). These potential limitations were overcome by using two measurements of microbial communities activity and therefore provide more reliable estimates of bacterial cell growth in this system. Data obtained using this approach and other bacterial activity estimates were compared. Incorporation of C-leucine into protein (Baath 1994; Chin-Leo and Kirchman 1988; 44 VOL. 104 NUTRIENT LIMITATION IN AN ALKALINE FEN Riemann and others 1990) was used as a supplemental measurement for estimating bacterial growth rates by evaluating the amount of label found in total cellular protein. Radiotracer incorporation into the DNA and protein pools in these assembleges has been shown to be proportional to the growth rate of the bacterial communities (Baath 1994; Tibbles and others 1992) and, therefore, of value to this study. Lipid incorporation of C-leucine was also examined as an indicator of environmental stress (Guckert and others 1992). MATERIALS AND METHODS" @default.
• W2153249512 created "2016-06-24" @default.
• W2153249512 creator A5009933366 @default.
• W2153249512 creator A5017457622 @default.
• W2153249512 date "2004-06-01" @default.
• W2153249512 modified "2023-09-27" @default.
• W2153249512 title "Estimation of Nutrient Limitation of Bacterial Activity in Temperate Alkaline Fen Sediments from Cedar Bog" @default.
• W2153249512 cites W1233072794 @default.
• W2153249512 cites W1503326979 @default.
• W2153249512 cites W1515985097 @default.
• W2153249512 cites W1551657140 @default.
• W2153249512 cites W157217960 @default.
• W2153249512 cites W1772456266 @default.
• W2153249512 cites W1968888328 @default.
• W2153249512 cites W1975415122 @default.
• W2153249512 cites W1984372558 @default.
• W2153249512 cites W1992873046 @default.
• W2153249512 cites W1995904586 @default.
• W2153249512 cites W2003716512 @default.
• W2153249512 cites W2005729889 @default.
• W2153249512 cites W2014178307 @default.
• W2153249512 cites W2018992702 @default.
• W2153249512 cites W2027929188 @default.
• W2153249512 cites W2028189434 @default.
• W2153249512 cites W2032470101 @default.
• W2153249512 cites W2052682182 @default.
• W2153249512 cites W2065863096 @default.
• W2153249512 cites W2081104534 @default.
• W2153249512 cites W2091495447 @default.
• W2153249512 cites W2091610578 @default.
• W2153249512 cites W2093057839 @default.
• W2153249512 cites W2106957978 @default.
• W2153249512 cites W2135610651 @default.
• W2153249512 cites W2136979585 @default.
• W2153249512 cites W2149967925 @default.
• W2153249512 cites W2168526533 @default.
• W2153249512 cites W223579488 @default.
• W2153249512 cites W2797924604 @default.
• W2153249512 cites W2995901967 @default.
• W2153249512 cites W48079217 @default.
• W2153249512 hasPublicationYear "2004" @default.
• W2153249512 type Work @default.
• W2153249512 sameAs 2153249512 @default.
• W2153249512 citedByCount "0" @default.
• W2153249512 crossrefType "journal-article" @default.
• W2153249512 hasAuthorship W2153249512A5009933366 @default.
• W2153249512 hasAuthorship W2153249512A5017457622 @default.
• W2153249512 hasConcept C107872376 @default.
• W2153249512 hasConcept C122284674 @default.
• W2153249512 hasConcept C142796444 @default.
• W2153249512 hasConcept C185592680 @default.
• W2153249512 hasConcept C18903297 @default.
• W2153249512 hasConcept C39432304 @default.
• W2153249512 hasConcept C53657456 @default.
• W2153249512 hasConcept C81461190 @default.
• W2153249512 hasConcept C86803240 @default.
• W2153249512 hasConceptScore W2153249512C107872376 @default.
• W2153249512 hasConceptScore W2153249512C122284674 @default.
• W2153249512 hasConceptScore W2153249512C142796444 @default.
• W2153249512 hasConceptScore W2153249512C185592680 @default.
• W2153249512 hasConceptScore W2153249512C18903297 @default.
• W2153249512 hasConceptScore W2153249512C39432304 @default.
• W2153249512 hasConceptScore W2153249512C53657456 @default.
• W2153249512 hasConceptScore W2153249512C81461190 @default.
• W2153249512 hasConceptScore W2153249512C86803240 @default.
• W2153249512 hasLocation W21532495121 @default.
• W2153249512 hasOpenAccess W2153249512 @default.
• W2153249512 hasPrimaryLocation W21532495121 @default.
• W2153249512 hasRelatedWork W2002995424 @default.
• W2153249512 hasRelatedWork W2006645668 @default.
• W2153249512 hasRelatedWork W2018245796 @default.
• W2153249512 hasRelatedWork W2023204192 @default.
• W2153249512 hasRelatedWork W20234597 @default.
• W2153249512 hasRelatedWork W2059052722 @default.
• W2153249512 hasRelatedWork W2080803032 @default.
• W2153249512 hasRelatedWork W2111114651 @default.
• W2153249512 hasRelatedWork W2112316420 @default.
• W2153249512 hasRelatedWork W2112943289 @default.
• W2153249512 hasRelatedWork W2125738481 @default.
• W2153249512 hasRelatedWork W2127611244 @default.
• W2153249512 hasRelatedWork W2129695632 @default.
• W2153249512 hasRelatedWork W2147751245 @default.
• W2153249512 hasRelatedWork W2157430690 @default.
• W2153249512 hasRelatedWork W2171175187 @default.
• W2153249512 hasRelatedWork W2291336217 @default.
• W2153249512 hasRelatedWork W2313141270 @default.
• W2153249512 hasRelatedWork W2467869946 @default.
• W2153249512 hasRelatedWork W2925048347 @default.
• W2153249512 isParatext "false" @default.
• W2153249512 isRetracted "false" @default.
• W2153249512 magId "2153249512" @default.
• W2153249512 workType "article" @default.