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- W2153295178 abstract "Increasing evidence indicates that mycobacteria may be involved in the aetiology and pathophysiology of sarcoidosis.To investigate the association between Mycobacterium tuberculosis complex infection and sarcoidosis.Mediastinal lymph node biopsy specimens (formalin-fixed, paraffin-embedded) from 52 Danish patients with sarcoidosis, 50 patients with mediastinal lymphadenopathy of other non-mycobacterial causes (negative controls) and 12 patients with histologically and/or culture-verified mycobacteriosis (positive controls) were included in the study. Biopsy samples were analysed for the presence of Mycobacterium tuberculosis complex by strand displacement assay and a subset of specimens were examined for bacterial rRNA by fluorescent in situ hybridisation using an eubacterial probe with general bacterial specificity (EUB338).One patient with sarcoidosis displayed a positive M. tuberculosis complex test. All negative controls were negative in the test and 5/12 patients with mycobacteriosis were positive in the test. We detected M. tuberculosis complex DNA in 10-year-old biopsy samples. Thirty-six samples were tested with the eubacterial probe; of these, 67% were positive with no difference between patients and controls.Our results do not support the hypothesis that M. tuberculosis complex infection is involved in the pathogenesis of sarcoidosis. However, we stress the importance of excluding mycobacteriosis in the diagnostic workup of sarcoidosis patients." @default.
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- W2153295178 date "2011-03-16" @default.
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- W2153295178 title "The continuing search for Mycobacterium tuberculosis involvement in sarcoidosis: a study on archival biopsy specimens" @default.
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- W2153295178 doi "https://doi.org/10.1111/j.1752-699x.2010.00205.x" @default.
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