FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153313883> ?p ?o ?g. }

• W2153313883 endingPage "1022" @default.
• W2153313883 startingPage "1013" @default.
• W2153313883 abstract "BACKGROUND HLA-G in biological fluids has been proposed to be useful as a tumor marker as both a diagnostic and prognostic factor. Most HLA-G measurement procedures are based on ELISA methods using highly specific antibodies. However, results of published studies are in conflict regarding the clinical utility and even the nature of HLA-G present in circulation. METHODS We collected 118 exudates, 94 from cancer patients and 24 from patients without tumors. We measured HLA-G concentrations by ELISA using MEM-G/9 or G233 as capture antibody. Samples were immunoprecipitated with an anti–HLA-G antibody and analyzed by Western blot using a different anti–HLA-G antibody. RESULTS Discrepancies in HLA-G concentrations in exudates were observed depending on what capture anti–HLA-G antibody was used for ELISA (r = 0.376). These discrepancies were not observed when the ELISAs were performed using culture supernatants from HLA-G1–transfected cells (r = 0.983). Immunoprecipitation and Western blot of cell culture supernatants with 2 different anti–HLA-G antibodies produced the typical band at 39 kDa assigned to HLA-G. When the immunoprecipitation and western blot were performed with exudates, however, there were bands at 53 kDa and 70–76 kDa, higher molecular weights than those usually assigned to HLA-G. These HLA-G–like molecules were associated with β2-microglobulin and could also form disulfide bridges with other HLA-G–like molecules. CONCLUSIONS The main HLA-G antigenic molecules in exudates are HLA-G–like complexes, a factor that should be considered when analyzing HLA-G in biological fluids." @default.
• W2153313883 created "2016-06-24" @default.
• W2153313883 creator A5017503015 @default.
• W2153313883 creator A5041694521 @default.
• W2153313883 creator A5051925857 @default.
• W2153313883 creator A5059531830 @default.
• W2153313883 creator A5063231825 @default.
• W2153313883 date "2011-07-01" @default.
• W2153313883 modified "2023-10-11" @default.
• W2153313883 title "Identification of Circulating Nonclassic Human Leukocyte Antigen G (HLA-G)–Like Molecules in Exudates" @default.
• W2153313883 cites W1575587378 @default.
• W2153313883 cites W1973533652 @default.
• W2153313883 cites W1979253523 @default.
• W2153313883 cites W1982946090 @default.
• W2153313883 cites W1988219173 @default.
• W2153313883 cites W1992010653 @default.
• W2153313883 cites W1994841387 @default.
• W2153313883 cites W1994937984 @default.
• W2153313883 cites W2006184289 @default.
• W2153313883 cites W2018219115 @default.
• W2153313883 cites W2021902701 @default.
• W2153313883 cites W2023923460 @default.
• W2153313883 cites W2026095495 @default.
• W2153313883 cites W2026125052 @default.
• W2153313883 cites W2030678127 @default.
• W2153313883 cites W2031509003 @default.
• W2153313883 cites W2032233271 @default.
• W2153313883 cites W2036810329 @default.
• W2153313883 cites W2038561197 @default.
• W2153313883 cites W2040155232 @default.
• W2153313883 cites W2047418972 @default.
• W2153313883 cites W2052439308 @default.
• W2153313883 cites W2057589452 @default.
• W2153313883 cites W2070700511 @default.
• W2153313883 cites W2071704959 @default.
• W2153313883 cites W2088514265 @default.
• W2153313883 cites W2089785223 @default.
• W2153313883 cites W2090887099 @default.
• W2153313883 cites W2091134590 @default.
• W2153313883 cites W2091880298 @default.
• W2153313883 cites W2099052946 @default.
• W2153313883 cites W2112169711 @default.
• W2153313883 cites W2137015486 @default.
• W2153313883 cites W2158829408 @default.
• W2153313883 cites W2160048269 @default.
• W2153313883 doi "https://doi.org/10.1373/clinchem.2010.159673" @default.
• W2153313883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21527645" @default.
• W2153313883 hasPublicationYear "2011" @default.
• W2153313883 type Work @default.
• W2153313883 sameAs 2153313883 @default.
• W2153313883 citedByCount "19" @default.
• W2153313883 countsByYear W21533138832012 @default.
• W2153313883 countsByYear W21533138832013 @default.
• W2153313883 countsByYear W21533138832014 @default.
• W2153313883 countsByYear W21533138832015 @default.
• W2153313883 countsByYear W21533138832016 @default.
• W2153313883 countsByYear W21533138832019 @default.
• W2153313883 countsByYear W21533138832020 @default.
• W2153313883 countsByYear W21533138832022 @default.
• W2153313883 crossrefType "journal-article" @default.
• W2153313883 hasAuthorship W2153313883A5017503015 @default.
• W2153313883 hasAuthorship W2153313883A5041694521 @default.
• W2153313883 hasAuthorship W2153313883A5051925857 @default.
• W2153313883 hasAuthorship W2153313883A5059531830 @default.
• W2153313883 hasAuthorship W2153313883A5063231825 @default.
• W2153313883 hasBestOaLocation W21533138831 @default.
• W2153313883 hasConcept C104317684 @default.
• W2153313883 hasConcept C147483822 @default.
• W2153313883 hasConcept C153911025 @default.
• W2153313883 hasConcept C159654299 @default.
• W2153313883 hasConcept C185592680 @default.
• W2153313883 hasConcept C188280979 @default.
• W2153313883 hasConcept C195616568 @default.
• W2153313883 hasConcept C203014093 @default.
• W2153313883 hasConcept C2776415932 @default.
• W2153313883 hasConcept C2780027760 @default.
• W2153313883 hasConcept C55493867 @default.
• W2153313883 hasConcept C71829478 @default.
• W2153313883 hasConcept C85265743 @default.
• W2153313883 hasConcept C86803240 @default.
• W2153313883 hasConceptScore W2153313883C104317684 @default.
• W2153313883 hasConceptScore W2153313883C147483822 @default.
• W2153313883 hasConceptScore W2153313883C153911025 @default.
• W2153313883 hasConceptScore W2153313883C159654299 @default.
• W2153313883 hasConceptScore W2153313883C185592680 @default.
• W2153313883 hasConceptScore W2153313883C188280979 @default.
• W2153313883 hasConceptScore W2153313883C195616568 @default.
• W2153313883 hasConceptScore W2153313883C203014093 @default.
• W2153313883 hasConceptScore W2153313883C2776415932 @default.
• W2153313883 hasConceptScore W2153313883C2780027760 @default.
• W2153313883 hasConceptScore W2153313883C55493867 @default.
• W2153313883 hasConceptScore W2153313883C71829478 @default.
• W2153313883 hasConceptScore W2153313883C85265743 @default.
• W2153313883 hasConceptScore W2153313883C86803240 @default.
• W2153313883 hasIssue "7" @default.
• W2153313883 hasLocation W21533138831 @default.
• W2153313883 hasLocation W21533138832 @default.
• W2153313883 hasOpenAccess W2153313883 @default.

• next