FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153315604> ?p ?o ?g. }

• W2153315604 endingPage "725" @default.
• W2153315604 startingPage "717" @default.
• W2153315604 abstract "Inhibin levels in the serum-free media of primary Sertoli cell-enriched (SCE) cultures were studied as a function of time and hormonal treatment. SCE cultures were established from 20-day-old rats and maintained in SF media supplemented with insulin, transferrin, epidermal growth factor (EGF), and bacitracin. Radioimmunoassayable inhibin was measured using an antibody directed against a synthetic porcine inhibin-α (plα) and measured using this same synthetic peptide as well as a highly purified ovine inhibin standard. Results of these determinations are expressed in terms of a synthetic peptide as femtomoles of pIα-(l–26)-G-Y-per ml/106 cells. Inhibin levels (±SEM) that accumulated at this rate in media from control cultures were 184.9 ± 6.1 and 167.4 ± 5.2 on days 0–4 and 4–8, respectively. When FSH (oFSH 17; 1–1000 ng/ml) was added, a dose-dependent increase in inhibin levels was significant at all time points beyond the initial 24 h. The simultaneous addition of 2 × 10-7 M testosterone (T) with low doses of FSH suppressed the inhibin response to FSH, but when T was combined with 300 ng/ml FSH, there was no effect of T on inhibin levels compared to FSH alone, regardless of time in culture. In spite of the modest effect of T, androstenedione (A; 2 × 10-13 to × 10-5 M) produced a dose-dependent suppression of inhibin levels. This inhibition was also observed at all doses of FSH. The action of A was not due to its conversion to estrogens, as 17β-estradiol had no effect on inhibin production by SCE cultures in either the presence or absence of FSH. The effect of EGF, a component of the basal serum-free medium, was next examined; it produced a 1.5-fold higher level of inhibin (188.7 ± 9.5) compared to cultures without EGF (135.6 ± 5.0). When SCE cultures were plated with FSH plus EGF, the stimulation of inhibin levels was additive. We conclude that in Sertoli cell cultures established from immature rats (1) the accumulation of inhibin in medium declines from 90 fmol/106 cells-day (initial 24 h) to 40–50 fmol/106 cells-day (over the first 48 h) and continues to accumulate in the medium for 8 days of culture; (2) FSH regulates the production of inhibin by Sertoli cells; the best dose response is observed over a 3-day period; (3) has a more marked suppressive effect on inhibin accumulation than does T; and (4) EGF stimulates basal inhibin production, and its effects are additive with those of FSH. (Endocrinology122: 717–725, 1988)" @default.
• W2153315604 created "2016-06-24" @default.
• W2153315604 creator A5002762738 @default.
• W2153315604 creator A5013808498 @default.
• W2153315604 creator A5027647881 @default.
• W2153315604 creator A5070748104 @default.
• W2153315604 date "1988-02-01" @default.
• W2153315604 modified "2023-09-27" @default.
• W2153315604 title "Inhibin Production by Primary Sertoli Cell-Enriched Cultures: Regulation by Follicle-Stimulating Hormone, Androgens, and Epidermal Growth Factor*" @default.
• W2153315604 cites W1936333617 @default.
• W2153315604 cites W1968036213 @default.
• W2153315604 cites W1974167869 @default.
• W2153315604 cites W1979472843 @default.
• W2153315604 cites W1982057209 @default.
• W2153315604 cites W1984484050 @default.
• W2153315604 cites W1985398262 @default.
• W2153315604 cites W1993565704 @default.
• W2153315604 cites W1996012010 @default.
• W2153315604 cites W1999763734 @default.
• W2153315604 cites W2010522639 @default.
• W2153315604 cites W2012779492 @default.
• W2153315604 cites W2036523229 @default.
• W2153315604 cites W2048491470 @default.
• W2153315604 cites W2049198626 @default.
• W2153315604 cites W2050880787 @default.
• W2153315604 cites W2058009234 @default.
• W2153315604 cites W2058915390 @default.
• W2153315604 cites W2064982147 @default.
• W2153315604 cites W2070175415 @default.
• W2153315604 cites W2075583064 @default.
• W2153315604 cites W2077120844 @default.
• W2153315604 cites W2079198737 @default.
• W2153315604 cites W2085828113 @default.
• W2153315604 cites W2093943347 @default.
• W2153315604 cites W2102076137 @default.
• W2153315604 cites W2109386389 @default.
• W2153315604 cites W2121970442 @default.
• W2153315604 cites W2124956283 @default.
• W2153315604 cites W2136195122 @default.
• W2153315604 cites W2409452046 @default.
• W2153315604 cites W2423005646 @default.
• W2153315604 cites W45572950 @default.
• W2153315604 cites W2789161292 @default.
• W2153315604 doi "https://doi.org/10.1210/endo-122-2-717" @default.
• W2153315604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3123203" @default.
• W2153315604 hasPublicationYear "1988" @default.
• W2153315604 type Work @default.
• W2153315604 sameAs 2153315604 @default.
• W2153315604 citedByCount "113" @default.
• W2153315604 countsByYear W21533156042015 @default.
• W2153315604 countsByYear W21533156042016 @default.
• W2153315604 countsByYear W21533156042022 @default.
• W2153315604 crossrefType "journal-article" @default.
• W2153315604 hasAuthorship W2153315604A5002762738 @default.
• W2153315604 hasAuthorship W2153315604A5013808498 @default.
• W2153315604 hasAuthorship W2153315604A5027647881 @default.
• W2153315604 hasAuthorship W2153315604A5070748104 @default.
• W2153315604 hasConcept C123765429 @default.
• W2153315604 hasConcept C126322002 @default.
• W2153315604 hasConcept C134018914 @default.
• W2153315604 hasConcept C170493617 @default.
• W2153315604 hasConcept C185592680 @default.
• W2153315604 hasConcept C2775960820 @default.
• W2153315604 hasConcept C2776362946 @default.
• W2153315604 hasConcept C2776683971 @default.
• W2153315604 hasConcept C2777911890 @default.
• W2153315604 hasConcept C2778575703 @default.
• W2153315604 hasConcept C2778782382 @default.
• W2153315604 hasConcept C2779279165 @default.
• W2153315604 hasConcept C2779279991 @default.
• W2153315604 hasConcept C2781068068 @default.
• W2153315604 hasConcept C71315377 @default.
• W2153315604 hasConcept C71924100 @default.
• W2153315604 hasConcept C86803240 @default.
• W2153315604 hasConceptScore W2153315604C123765429 @default.
• W2153315604 hasConceptScore W2153315604C126322002 @default.
• W2153315604 hasConceptScore W2153315604C134018914 @default.
• W2153315604 hasConceptScore W2153315604C170493617 @default.
• W2153315604 hasConceptScore W2153315604C185592680 @default.
• W2153315604 hasConceptScore W2153315604C2775960820 @default.
• W2153315604 hasConceptScore W2153315604C2776362946 @default.
• W2153315604 hasConceptScore W2153315604C2776683971 @default.
• W2153315604 hasConceptScore W2153315604C2777911890 @default.
• W2153315604 hasConceptScore W2153315604C2778575703 @default.
• W2153315604 hasConceptScore W2153315604C2778782382 @default.
• W2153315604 hasConceptScore W2153315604C2779279165 @default.
• W2153315604 hasConceptScore W2153315604C2779279991 @default.
• W2153315604 hasConceptScore W2153315604C2781068068 @default.
• W2153315604 hasConceptScore W2153315604C71315377 @default.
• W2153315604 hasConceptScore W2153315604C71924100 @default.
• W2153315604 hasConceptScore W2153315604C86803240 @default.
• W2153315604 hasIssue "2" @default.
• W2153315604 hasLocation W21533156041 @default.
• W2153315604 hasLocation W21533156042 @default.
• W2153315604 hasOpenAccess W2153315604 @default.
• W2153315604 hasPrimaryLocation W21533156041 @default.
• W2153315604 hasRelatedWork W1988486434 @default.
• W2153315604 hasRelatedWork W2008511598 @default.

• next