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• W2153368281 abstract "Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further fractionated using anion-exchange chromatography and fractions pooled on the basis of containing (1) protease activity, (2) a prominent non-protease peptide and (3) material which did not bind to the column. A protection trial showed that only the protease-enriched material conferred protective immunity and this was comparable to that observed in a TSBP-immunized positive control group. Immunization stimulated a marked IgG response with the IgG2 isotype predominating." @default.
• W2153368281 created "2016-06-24" @default.
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• W2153368281 date "2005-04-01" @default.
• W2153368281 modified "2023-10-10" @default.
• W2153368281 title "Protection induced by vaccinating sheep with a thiol-binding extract of Haemonchus contortus membranes is associated with its protease components" @default.
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• W2153368281 doi "https://doi.org/10.1111/j.1365-3024.2005.00750.x" @default.
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