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• W2153411841 abstract "Subcritical water has been recently employed as the mobile phase to eliminate the use of organic solvents in reversed-phase liquid chromatography. Although the influence of temperature on retention in subcritical water chromatography has been reported, the temperature effect on peak width and column efficiency has not yet been quantitatively studied. In this work, several polar and chlorinated compounds are separated using pure subcritical water on Zorbax RX-C8, PRP-1 (polystyrene-divinylbenzene), Hypersil ODS, and ZirChrom-polybutadiene columns. Isothermal separations are performed at temperatures ranging from 60°C to 160°C. The retention time and peak width of analytes are reduced with increasing temperature. However, the column efficiency is either improved or almost unchanged with the increasing temperature in the low-temperature range (lower than the 100°C to 120°C range), but it is decreased when temperature is further raised in the hightemperature range (higher than the 100°C to 120°C range). Therefore, a maximum in column efficiency is obtained at temperatures within the 100°C to 120°C range in most cases." @default.
• W2153411841 created "2016-06-24" @default.
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• W2153411841 date "2002-02-01" @default.
• W2153411841 modified "2023-10-04" @default.
• W2153411841 title "Temperature Effect on Peak Width and Column Efficiency in Subcritical Water Chromatography" @default.
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• W2153411841 doi "https://doi.org/10.1093/chromsci/40.2.107" @default.
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