Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153420005> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2153420005 endingPage "306" @default.
- W2153420005 startingPage "301" @default.
- W2153420005 abstract "Aim: Owing to the widespread use of antibiotic drugs in both human and animals, we thought it would be important to determine the effect of these on lactoperoxidase (LPO; E.C. 1.11.1.7) activity during lactation. The in vitro effects of gentamicin sulfate and ampicillin sodium drugs on bovine LPO enzyme activity in breastmilk were investigated. Materials and Methods: LPO was purified with Amberlite CG 50 resin, CM Sephadex C-50 ion-exchange chromatography, and Sephadex G-100 gel filtration chromatography from skimmed bovine milk. Rz(A412/A280) value for the purified LPO was found to be 0.8. Inhibition or activation effects of the drugs on LPO enzyme were determined using 2,2 1 -azino–bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (ABTS) as a chromogenic substrate at pH = 6.0. Results: The purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). I50 values of gentamicin sulfate and ampicillin sodium drugs exhibiting inhibition effects were obtained by drawing % Activity- [drugs] graphs. The obtained I50 values of gentamicin sulfate and ampicillin sodium were 3.71 mM and 0.89 mM, respectively. Ki constants of the drugs were found by means of Lineweaver-Burk graphs. The obtained Ki constants for gentamicin sulfate and ampicillin sodium were 4.36 ± 3.122 mM and 0.473 ± 0.099 mM, respectively, and they were competitive inhibitors. Conclusions: Use of antibiotics may worsen the LPO activity in milk during lactation. If these antibiotic drugs are required during lactation, their dosage should be carefully determined to decrease the side effects." @default.
- W2153420005 created "2016-06-24" @default.
- W2153420005 creator A5001624661 @default.
- W2153420005 creator A5055644110 @default.
- W2153420005 creator A5061049724 @default.
- W2153420005 creator A5074851120 @default.
- W2153420005 date "2006-05-01" @default.
- W2153420005 modified "2023-10-18" @default.
- W2153420005 title "In vitro effects of some antibiotic drugs on bovine lactoperoxidase enzyme" @default.
- W2153420005 cites W137665672 @default.
- W2153420005 cites W1602589000 @default.
- W2153420005 cites W1775749144 @default.
- W2153420005 cites W1835162629 @default.
- W2153420005 cites W1887495265 @default.
- W2153420005 cites W1970219543 @default.
- W2153420005 cites W1987571383 @default.
- W2153420005 cites W1999770795 @default.
- W2153420005 cites W2014607435 @default.
- W2153420005 cites W2024339664 @default.
- W2153420005 cites W2072228771 @default.
- W2153420005 cites W2084609399 @default.
- W2153420005 cites W2085594302 @default.
- W2153420005 cites W2100837269 @default.
- W2153420005 cites W2106186879 @default.
- W2153420005 cites W2150006743 @default.
- W2153420005 cites W2407974802 @default.
- W2153420005 cites W2411688119 @default.
- W2153420005 cites W2558636281 @default.
- W2153420005 hasPublicationYear "2006" @default.
- W2153420005 type Work @default.
- W2153420005 sameAs 2153420005 @default.
- W2153420005 citedByCount "2" @default.
- W2153420005 countsByYear W21534200052015 @default.
- W2153420005 crossrefType "journal-article" @default.
- W2153420005 hasAuthorship W2153420005A5001624661 @default.
- W2153420005 hasAuthorship W2153420005A5055644110 @default.
- W2153420005 hasAuthorship W2153420005A5061049724 @default.
- W2153420005 hasAuthorship W2153420005A5074851120 @default.
- W2153420005 hasConcept C162008176 @default.
- W2153420005 hasConcept C163994747 @default.
- W2153420005 hasConcept C181199279 @default.
- W2153420005 hasConcept C185592680 @default.
- W2153420005 hasConcept C201956729 @default.
- W2153420005 hasConcept C2775832221 @default.
- W2153420005 hasConcept C2776021129 @default.
- W2153420005 hasConcept C2778913249 @default.
- W2153420005 hasConcept C2910288237 @default.
- W2153420005 hasConcept C43617362 @default.
- W2153420005 hasConcept C501593827 @default.
- W2153420005 hasConcept C55493867 @default.
- W2153420005 hasConcept C71924100 @default.
- W2153420005 hasConceptScore W2153420005C162008176 @default.
- W2153420005 hasConceptScore W2153420005C163994747 @default.
- W2153420005 hasConceptScore W2153420005C181199279 @default.
- W2153420005 hasConceptScore W2153420005C185592680 @default.
- W2153420005 hasConceptScore W2153420005C201956729 @default.
- W2153420005 hasConceptScore W2153420005C2775832221 @default.
- W2153420005 hasConceptScore W2153420005C2776021129 @default.
- W2153420005 hasConceptScore W2153420005C2778913249 @default.
- W2153420005 hasConceptScore W2153420005C2910288237 @default.
- W2153420005 hasConceptScore W2153420005C43617362 @default.
- W2153420005 hasConceptScore W2153420005C501593827 @default.
- W2153420005 hasConceptScore W2153420005C55493867 @default.
- W2153420005 hasConceptScore W2153420005C71924100 @default.
- W2153420005 hasIssue "5" @default.
- W2153420005 hasLocation W21534200051 @default.
- W2153420005 hasOpenAccess W2153420005 @default.
- W2153420005 hasPrimaryLocation W21534200051 @default.
- W2153420005 hasRelatedWork W1508638897 @default.
- W2153420005 hasRelatedWork W1511097185 @default.
- W2153420005 hasRelatedWork W1641732004 @default.
- W2153420005 hasRelatedWork W1862874943 @default.
- W2153420005 hasRelatedWork W1976392811 @default.
- W2153420005 hasRelatedWork W1982423122 @default.
- W2153420005 hasRelatedWork W1988452686 @default.
- W2153420005 hasRelatedWork W1994960299 @default.
- W2153420005 hasRelatedWork W2006661331 @default.
- W2153420005 hasRelatedWork W2020216272 @default.
- W2153420005 hasRelatedWork W2026920058 @default.
- W2153420005 hasRelatedWork W2032607711 @default.
- W2153420005 hasRelatedWork W2054286701 @default.
- W2153420005 hasRelatedWork W2075159361 @default.
- W2153420005 hasRelatedWork W2109816941 @default.
- W2153420005 hasRelatedWork W2116685962 @default.
- W2153420005 hasRelatedWork W2122757055 @default.
- W2153420005 hasRelatedWork W2235615045 @default.
- W2153420005 hasRelatedWork W2400100482 @default.
- W2153420005 hasRelatedWork W828117342 @default.
- W2153420005 hasVolume "36" @default.
- W2153420005 isParatext "false" @default.
- W2153420005 isRetracted "false" @default.
- W2153420005 magId "2153420005" @default.
- W2153420005 workType "article" @default.