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- W2153446938 abstract "PURPUSE: To identify and characterize extended-spectrum beta-lactamases (ESBLs) present in the chromosomal AmpC-producing enterobacterial isolates. METHODS: Screening for genes encoding ESBLs (blaPER-1, blaTEM, blaSHV, blaCTX-M, blaVEB-1 and blaGES-1) was carried out by PCR amplification with specific primers in 37 non-duplicate, clinically relevant ESBL-producing isolates including 19 Citrobacter freundii, 9 Enterobacter cloacae, 5 Serratia marcescens, 2 Enterobacter aerogenes, 1 Morganella morganii and 1 Providencia rettgeri. For isolates with PCR-positive results, sequencing was performed. Susceptibility to antimicrobials was determined by standard disk diffusion or Etest procedures. RESULTS: The 37 chromosomal AmpC-producing Enterobacteriaceae were found to coproduce the following ESBLs: 14 (37.8%) CTX-M-3, nine (24.3%) TEM-3, eight (21.6%) SHV-2, four (10.8%) CTX-M-15, one (2.7%) TEM-15 and one (2.7%) PER-1. Sixteen isolates (43.2%) also carried blaTEM-1, and one of them carried blaSHV-1 as well. In vitro, all isolates were susceptible to imipenem. Susceptibility to other drugs was as follows: 78 % for ciprofloxacin, 43% for amikacin and 32 % for gentamicin. Associated resistance to amikacin and ciprofloxacin was observed most frequently among CTX-M-positive isolates. CONCLUSIONS: The most prevalent ESBLs were CTXM enzymes (CTX-M-3 and CTX-M-15) followed by TEM-3 and SHV-2. This is the first report of TEM-15 and PER-1-producing Enterobacteriaceae in Bulgaria." @default.
- W2153446938 created "2016-06-24" @default.
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- W2153446938 date "2008-01-01" @default.
- W2153446938 modified "2023-09-27" @default.
- W2153446938 title "Original Contribution IDENTIFICATION OF SHV-2, TEM-3, TEM-15, CTX-M-3, CTX-M-15 AND PER-1 EXTENDED-SPECTRUM BETA-LACTAMASES IN CHROMOSOMAL AMPC-PRODUCING ENTEROBACTERIACEAE ISOLATES FROM CANCER PATIENTS IN BULGARIA" @default.
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