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- W2153483399 abstract "Abstract A simple and clean adsorption/desorption process was proposed for recovering Acinetobacter radio resisten s lipase from fermentation broth. The adsorbent used was n ‐hexadecane coated on a hydrophobic nonwoven fabric (NWF). n ‐Hexadecane has a melting point of 16–18 °C, and its affinity for lipase decreases markedly from liquid to solid state. Accordingly, performing the adsorption and desorption above and below, respectively, the melting point would need no extraneous materials for separation. The adsorption isotherms at various temperatures were found to follow the Langmuir model. Simulation of the batch adsorption/desorption process showed that there exists an optimal amount of adsorbent for both concentration factor and enzyme recovery; the process is restrained by equilibrium. The performance of column adsorption/desorption could also be simulated using the adsorption isotherm, and it was shown that the concentration factor was proportional to the amount of adsorbent used. The benefits of this process include easy preparation of adsorbent, low operational cost, no extraneous materials needed, negligible enzyme denaturation, high efficiency, and simple process simulation." @default.
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- W2153483399 date "2003-01-01" @default.
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- W2153483399 title "Recovery of Acinetobacter radioresistens Lipase by Hydrophobic Adsorption to n-Hexadecane Coated on Nonwoven Fabric" @default.
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- W2153483399 doi "https://doi.org/10.1021/bp020124a" @default.
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