Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153543145> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2153543145 endingPage "403" @default.
- W2153543145 startingPage "399" @default.
- W2153543145 abstract "Abstract Background: Trisomy 21 or Down syndrome (DS) is the most common aneuploidy disorder. Fetal karyotypic analysis remains the criterion standard for prenatal diagnosis of DS, although the method is time consuming and requires skilled personnel. Real-time quantitative polymerase chain reaction (qPCR) can be used to determine a difference in the amount of gene copy by calculation of the difference between the cycle threshold (ΔCT) of a tested gene and a reference gene. Objectives: To develop a rapid qPCR diagnostic method for trisomy 21. Methods: Ten DS patients with the known karyotype of trisomy 21 were enrolled. Their parents were included as controls. D21S11 locus on chromosome 21 and SM locus on chromosome 16 from each subject were amplified by qPCR. The D21S11/SM ΔCT and 2 -ΔΔCT values were compared between DS patients and their parents. Results: The D21S11/SM ΔCT values of the DS patients were higher than their respective controls except for one family. The mean 2 -ΔΔCT value between patients and mothers was 1.88 ± 0.95 (95% CI 1.20-2.56), and between fathers and mothers as controls was 1.06 ± 0.68 (95% CI 0.58-1.54). Conclusion: The diagnostic method of trisomy 21 by using qPCR is feasible, although false negative results may occur. Using more index genes is recommended to increase the sensitivity and specificity." @default.
- W2153543145 created "2016-06-24" @default.
- W2153543145 creator A5017296127 @default.
- W2153543145 creator A5018079356 @default.
- W2153543145 creator A5030025269 @default.
- W2153543145 creator A5051418370 @default.
- W2153543145 creator A5065383271 @default.
- W2153543145 date "2014-06-01" @default.
- W2153543145 modified "2023-10-14" @default.
- W2153543145 title "Brief communication (Original). Rapid diagnosis of trisomy 21 by relative gene copy using real-time quantitative polymerase chain reaction" @default.
- W2153543145 cites W1957531924 @default.
- W2153543145 cites W1970291363 @default.
- W2153543145 cites W1973651439 @default.
- W2153543145 cites W2017399145 @default.
- W2153543145 cites W2018594718 @default.
- W2153543145 cites W2042691801 @default.
- W2153543145 cites W2048536697 @default.
- W2153543145 cites W2079241422 @default.
- W2153543145 cites W2088559557 @default.
- W2153543145 cites W2094067621 @default.
- W2153543145 cites W2107277218 @default.
- W2153543145 cites W2139714132 @default.
- W2153543145 cites W2159180279 @default.
- W2153543145 cites W2164216363 @default.
- W2153543145 cites W2185511594 @default.
- W2153543145 cites W4241487444 @default.
- W2153543145 doi "https://doi.org/10.5372/1905-7415.0803.306" @default.
- W2153543145 hasPublicationYear "2014" @default.
- W2153543145 type Work @default.
- W2153543145 sameAs 2153543145 @default.
- W2153543145 citedByCount "0" @default.
- W2153543145 crossrefType "journal-article" @default.
- W2153543145 hasAuthorship W2153543145A5017296127 @default.
- W2153543145 hasAuthorship W2153543145A5018079356 @default.
- W2153543145 hasAuthorship W2153543145A5030025269 @default.
- W2153543145 hasAuthorship W2153543145A5051418370 @default.
- W2153543145 hasAuthorship W2153543145A5065383271 @default.
- W2153543145 hasBestOaLocation W21535431451 @default.
- W2153543145 hasConcept C104317684 @default.
- W2153543145 hasConcept C137555145 @default.
- W2153543145 hasConcept C172680121 @default.
- W2153543145 hasConcept C2776229224 @default.
- W2153543145 hasConcept C2778258057 @default.
- W2153543145 hasConcept C2779234561 @default.
- W2153543145 hasConcept C2779672484 @default.
- W2153543145 hasConcept C2779954681 @default.
- W2153543145 hasConcept C30481170 @default.
- W2153543145 hasConcept C48023723 @default.
- W2153543145 hasConcept C49105822 @default.
- W2153543145 hasConcept C53226629 @default.
- W2153543145 hasConcept C54355233 @default.
- W2153543145 hasConcept C71924100 @default.
- W2153543145 hasConcept C7602840 @default.
- W2153543145 hasConcept C84597430 @default.
- W2153543145 hasConcept C86803240 @default.
- W2153543145 hasConceptScore W2153543145C104317684 @default.
- W2153543145 hasConceptScore W2153543145C137555145 @default.
- W2153543145 hasConceptScore W2153543145C172680121 @default.
- W2153543145 hasConceptScore W2153543145C2776229224 @default.
- W2153543145 hasConceptScore W2153543145C2778258057 @default.
- W2153543145 hasConceptScore W2153543145C2779234561 @default.
- W2153543145 hasConceptScore W2153543145C2779672484 @default.
- W2153543145 hasConceptScore W2153543145C2779954681 @default.
- W2153543145 hasConceptScore W2153543145C30481170 @default.
- W2153543145 hasConceptScore W2153543145C48023723 @default.
- W2153543145 hasConceptScore W2153543145C49105822 @default.
- W2153543145 hasConceptScore W2153543145C53226629 @default.
- W2153543145 hasConceptScore W2153543145C54355233 @default.
- W2153543145 hasConceptScore W2153543145C71924100 @default.
- W2153543145 hasConceptScore W2153543145C7602840 @default.
- W2153543145 hasConceptScore W2153543145C84597430 @default.
- W2153543145 hasConceptScore W2153543145C86803240 @default.
- W2153543145 hasIssue "3" @default.
- W2153543145 hasLocation W21535431451 @default.
- W2153543145 hasOpenAccess W2153543145 @default.
- W2153543145 hasPrimaryLocation W21535431451 @default.
- W2153543145 hasRelatedWork W1973651439 @default.
- W2153543145 hasRelatedWork W1989347152 @default.
- W2153543145 hasRelatedWork W1998404587 @default.
- W2153543145 hasRelatedWork W2044362363 @default.
- W2153543145 hasRelatedWork W2153543145 @default.
- W2153543145 hasRelatedWork W2388961464 @default.
- W2153543145 hasRelatedWork W2393047937 @default.
- W2153543145 hasRelatedWork W2461857953 @default.
- W2153543145 hasRelatedWork W271066145 @default.
- W2153543145 hasRelatedWork W2775976160 @default.
- W2153543145 hasVolume "8" @default.
- W2153543145 isParatext "false" @default.
- W2153543145 isRetracted "false" @default.
- W2153543145 magId "2153543145" @default.
- W2153543145 workType "article" @default.