Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153565075> ?p ?o ?g. }
- W2153565075 endingPage "4987" @default.
- W2153565075 startingPage "4979" @default.
- W2153565075 abstract "Herpesviruses accomplish DNA replication either by expressing their own deoxyribonucleotide biosynthetic genes or by stimulating the expression of the corresponding cellular genes. Cytomegalovirus (CMV) has adopted the latter strategy to allow efficient replication in quiescent cells. In the present report, we show that murine CMV (MCMV) infection of quiescent fibroblasts induces both mRNA and protein corresponding to the cellular thymidylate synthase (TS) gene, which encodes the enzyme that catalyzes the de novo synthesis of thymidylic acid. The increase in TS gene expression was due to an increase in gene transcription, since the activity of a reporter gene driven by the mouse TS promoter was induced following MCMV infection. Mutagenesis of the potential E2F-responsive element immediately upstream from the TS essential promoter region abolished the virus-mediated stimulation of the TS promoter, suggesting that the transactivating activity of MCMV infection was E2F dependent. Cotransfection experiments revealed that expression of the viral immediate-early 1 protein was sufficient to mediate the increase in TS promoter activity. Finally, MCMV replication and viral DNA synthesis were found to be inhibited by ZD1694, a quinazoline-based folate analog that inhibits TS activity. These results demonstrate that upregulation of cellular TS expression is required for efficient MCMV replication in quiescent cells." @default.
- W2153565075 created "2016-06-24" @default.
- W2153565075 creator A5005108228 @default.
- W2153565075 creator A5024383373 @default.
- W2153565075 creator A5026529883 @default.
- W2153565075 creator A5041453870 @default.
- W2153565075 creator A5042560289 @default.
- W2153565075 creator A5062786874 @default.
- W2153565075 creator A5065623232 @default.
- W2153565075 creator A5084389949 @default.
- W2153565075 date "2000-06-01" @default.
- W2153565075 modified "2023-09-26" @default.
- W2153565075 title "Murine Cytomegalovirus Stimulates Cellular Thymidylate Synthase Gene Expression in Quiescent Cells and Requires the Enzyme for Replication" @default.
- W2153565075 cites W1485678634 @default.
- W2153565075 cites W1600236870 @default.
- W2153565075 cites W1608297032 @default.
- W2153565075 cites W1614146403 @default.
- W2153565075 cites W1621571238 @default.
- W2153565075 cites W1754219621 @default.
- W2153565075 cites W1826236781 @default.
- W2153565075 cites W1847606687 @default.
- W2153565075 cites W1862249071 @default.
- W2153565075 cites W1870943906 @default.
- W2153565075 cites W1943526598 @default.
- W2153565075 cites W1950016994 @default.
- W2153565075 cites W1957815482 @default.
- W2153565075 cites W1971114615 @default.
- W2153565075 cites W1971876556 @default.
- W2153565075 cites W1980645718 @default.
- W2153565075 cites W1983668143 @default.
- W2153565075 cites W1989998960 @default.
- W2153565075 cites W1994619256 @default.
- W2153565075 cites W1996992659 @default.
- W2153565075 cites W2010880776 @default.
- W2153565075 cites W2033245328 @default.
- W2153565075 cites W2033516360 @default.
- W2153565075 cites W2040686546 @default.
- W2153565075 cites W2040850705 @default.
- W2153565075 cites W2046516455 @default.
- W2153565075 cites W2047264397 @default.
- W2153565075 cites W2051403632 @default.
- W2153565075 cites W2058106399 @default.
- W2153565075 cites W2060333964 @default.
- W2153565075 cites W2063825413 @default.
- W2153565075 cites W2069023071 @default.
- W2153565075 cites W2095495999 @default.
- W2153565075 cites W2097033810 @default.
- W2153565075 cites W2098207727 @default.
- W2153565075 cites W2101508260 @default.
- W2153565075 cites W2108367007 @default.
- W2153565075 cites W2109293649 @default.
- W2153565075 cites W2110670582 @default.
- W2153565075 cites W2113156630 @default.
- W2153565075 cites W2114659793 @default.
- W2153565075 cites W2114739240 @default.
- W2153565075 cites W2116761701 @default.
- W2153565075 cites W2118391342 @default.
- W2153565075 cites W2119978969 @default.
- W2153565075 cites W2121760334 @default.
- W2153565075 cites W2124716540 @default.
- W2153565075 cites W2130413473 @default.
- W2153565075 cites W2131232623 @default.
- W2153565075 cites W2138440094 @default.
- W2153565075 cites W2145082982 @default.
- W2153565075 cites W2148382823 @default.
- W2153565075 cites W2153934725 @default.
- W2153565075 cites W2156924638 @default.
- W2153565075 cites W2161086529 @default.
- W2153565075 cites W2170308653 @default.
- W2153565075 cites W2189451570 @default.
- W2153565075 cites W2189484135 @default.
- W2153565075 cites W2283848595 @default.
- W2153565075 cites W2418251045 @default.
- W2153565075 cites W4240285463 @default.
- W2153565075 cites W4251126739 @default.
- W2153565075 cites W4251188023 @default.
- W2153565075 cites W4252766809 @default.
- W2153565075 doi "https://doi.org/10.1128/jvi.74.11.4979-4987.2000" @default.
- W2153565075 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/110849" @default.
- W2153565075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10799571" @default.
- W2153565075 hasPublicationYear "2000" @default.
- W2153565075 type Work @default.
- W2153565075 sameAs 2153565075 @default.
- W2153565075 citedByCount "45" @default.
- W2153565075 countsByYear W21535650752012 @default.
- W2153565075 countsByYear W21535650752013 @default.
- W2153565075 countsByYear W21535650752014 @default.
- W2153565075 countsByYear W21535650752015 @default.
- W2153565075 countsByYear W21535650752016 @default.
- W2153565075 countsByYear W21535650752018 @default.
- W2153565075 countsByYear W21535650752020 @default.
- W2153565075 countsByYear W21535650752021 @default.
- W2153565075 crossrefType "journal-article" @default.
- W2153565075 hasAuthorship W2153565075A5005108228 @default.
- W2153565075 hasAuthorship W2153565075A5024383373 @default.
- W2153565075 hasAuthorship W2153565075A5026529883 @default.
- W2153565075 hasAuthorship W2153565075A5041453870 @default.
- W2153565075 hasAuthorship W2153565075A5042560289 @default.