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- W2153568228 abstract "Summary A simple method based upon polymerase chain reaction (PCR) was developed to detect mutant alleles of the gene encoding α 2‐tubulin, which confers recessive resistance to tubulin‐binding herbicides in Setaria viridis . Multiplex, bidirectional allele‐specific PCR (Mbi‐PASA) was shown to specifically and reliably detect the presence of all sensitive (Leu 136 , Thr 239 ) and resistant (Phe 136 , Ile 239 ) α 2‐tubulin alleles in a single reaction. Double‐blind analysis of 2000 S. viridis seedlings using seed bioassay and Mbi‐PASA confirmed that the presence of two mutant α 2‐tubulin alleles in a seedling was always associated with cross‐resistance to dinitroaniline and benzoic acid herbicides, sensitivity to a benzamide herbicide, and hypersensitivity to carbamate herbicides. No other resistance mechanism was detected in the S. viridis populations screened. Successful Mbi‐PASA genotyping was achieved with fresh and dried plant fragments from the field. Compared with bioassays, Mbi‐PASA is faster and more robust, removing the need for live plant material. It is the only way of detecting recessive resistance before resistant plants occur in a field. Mbi‐PASA can be performed with basic molecular biology laboratory equipment, and is suitable for high‐throughput genotyping adaptation. It is the tool of choice for resistance diagnosis in such cases where only a few recessive, target‐derived, genes control resistance to herbicides." @default.
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- W2153568228 date "2005-04-19" @default.
- W2153568228 modified "2023-10-16" @default.
- W2153568228 title "A single polymerase chain reaction-based assay for simultaneous detection of two mutations conferring resistance to tubulin-binding herbicides in Setaria viridis" @default.
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- W2153568228 doi "https://doi.org/10.1111/j.1365-3180.2005.00446.x" @default.
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