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- W2153588377 abstract "The effect of spore inoculum level and mycelia morphology on raw starch digesting amylase production by Aspergillus carbonarius in submerged culture was investigated. Shake flask cultures were inoculated directly with spore concentration range of 104 to 109spores/ml. Morphology was differentiated into, pellets, clumps, branched and free filaments. Results on the percentage morphological occurrences revealed that for all tested inoculum a gradual change in morphology was observed as innoculum level increased from 104 to 109spores/ml though pellets predominated. A decrease in the mean main and total hyphal length, mean hyphal growth unit and marginal increase in mean number of tips/mycelium was observed in the mycelia trees as inoculum levels increased from 104 to 109spores/ml. In the aggregated forms, there was a decrease in the mean area, perimeter, roughness and equivalent diameter while mean compactness increased as inoculum increased from 104 to 109 spores/ml. Irrespective of the predominating form a change in morphology was observed. RSDA production appeared to be related to spore inoculum level. Maximum yield was observed at fermentation inoculated with 104 and 105spores/ml where pellets were most dominant. The impact of spore inoculum size on the resulting mycelia morphology was marginal but had a significant effect on RSDA production.Key words: Aspergillus carbonarius, raw starch digesting amylase, fungal morphology and inoculum." @default.
- W2153588377 created "2016-06-24" @default.
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- W2153588377 date "2012-03-09" @default.
- W2153588377 modified "2023-09-25" @default.
- W2153588377 title "Characterization of morphological forms of Aspergillus carbonarius and the effect of inoculum size on raw starch digesting amylase (RSDA) production" @default.
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- W2153588377 doi "https://doi.org/10.5897/ajmr11.919" @default.
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