Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153683423> ?p ?o ?g. }
- W2153683423 endingPage "46" @default.
- W2153683423 startingPage "46" @default.
- W2153683423 abstract "Little is known regarding the pool of mobile genetic elements associated with the human gut microbiome. In this study we employed the culture independent TRACA system to isolate novel plasmids from the human gut microbiota, and a comparative metagenomic analysis to investigate the distribution and relative abundance of functions encoded by these plasmids in the human gut microbiome. Novel plasmids were acquired from the human gut microbiome, and homologous nucleotide sequences with high identity (>90%) to two plasmids (pTRACA10 and pTRACA22) were identified in the multiple human gut microbiomes analysed here. However, no homologous nucleotide sequences to these plasmids were identified in the murine gut or environmental metagenomes. Functions encoded by the plasmids pTRACA10 and pTRACA22 were found to be more prevalent in the human gut microbiome when compared to microbial communities from other environments. Among the most prevalent functions identified was a putative RelBE toxin-antitoxin (TA) addiction module, and subsequent analysis revealed that this was most closely related to putative TA modules from gut associated bacteria belonging to the Firmicutes. A broad phylogenetic distribution of RelE toxin genes was observed in gut associated bacterial species (Firmicutes, Bacteroidetes, Actinobacteria and Proteobacteria), but no RelE homologues were identified in gut associated archaeal species. We also provide indirect evidence for the horizontal transfer of these genes between bacterial species belonging to disparate phylogenetic divisions, namely Gram negative Proteobacteria and Gram positive species from the Firmicutes division. The application of a culture independent system to capture novel plasmids from the human gut mobile metagenome, coupled with subsequent comparative metagenomic analysis, highlighted the unexpected prevalence of plasmid encoded functions in the gut microbial ecosystem. In particular the increased relative abundance and broad phylogenetic distribution was identified for a putative RelBE toxin/antitoxin addiction module, a putative phosphohydrolase/phosphoesterase, and an ORF of unknown function. Our analysis also indicates that some plasmids or plasmid families are present in the gut microbiomes of geographically isolated human hosts with a broad global distribution (America, Japan and Europe), and are potentially unique to the human gut microbiome. Further investigation of the plasmid population associated with the human gut is likely to provide important insights into the development, functioning and evolution of the human gut microbiota." @default.
- W2153683423 created "2016-06-24" @default.
- W2153683423 creator A5020253377 @default.
- W2153683423 creator A5021701065 @default.
- W2153683423 creator A5027399260 @default.
- W2153683423 date "2010-01-01" @default.
- W2153683423 modified "2023-10-11" @default.
- W2153683423 title "Comparative metagenomic analysis of plasmid encoded functions in the human gut microbiome" @default.
- W2153683423 cites W1483648977 @default.
- W2153683423 cites W1532814292 @default.
- W2153683423 cites W1562303068 @default.
- W2153683423 cites W1979696774 @default.
- W2153683423 cites W1980914602 @default.
- W2153683423 cites W1990080521 @default.
- W2153683423 cites W2011210667 @default.
- W2153683423 cites W2015318086 @default.
- W2153683423 cites W2015442594 @default.
- W2153683423 cites W2022255652 @default.
- W2153683423 cites W2032870665 @default.
- W2153683423 cites W2035779227 @default.
- W2153683423 cites W2037833069 @default.
- W2153683423 cites W2044915317 @default.
- W2153683423 cites W2047805950 @default.
- W2153683423 cites W2055126178 @default.
- W2153683423 cites W2056536052 @default.
- W2153683423 cites W2057802960 @default.
- W2153683423 cites W2058313830 @default.
- W2153683423 cites W2059105112 @default.
- W2153683423 cites W2075770792 @default.
- W2153683423 cites W2078729905 @default.
- W2153683423 cites W2081084109 @default.
- W2153683423 cites W2088161730 @default.
- W2153683423 cites W2090925778 @default.
- W2153683423 cites W2093814623 @default.
- W2153683423 cites W2102114757 @default.
- W2153683423 cites W2103784576 @default.
- W2153683423 cites W2104688104 @default.
- W2153683423 cites W2105837832 @default.
- W2153683423 cites W2107986672 @default.
- W2153683423 cites W2113601822 @default.
- W2153683423 cites W2117130914 @default.
- W2153683423 cites W2117727476 @default.
- W2153683423 cites W2118001436 @default.
- W2153683423 cites W2123082123 @default.
- W2153683423 cites W2124937554 @default.
- W2153683423 cites W2126573196 @default.
- W2153683423 cites W2126809954 @default.
- W2153683423 cites W2127415562 @default.
- W2153683423 cites W2137042419 @default.
- W2153683423 cites W2137956358 @default.
- W2153683423 cites W2138931362 @default.
- W2153683423 cites W2141296695 @default.
- W2153683423 cites W2141365940 @default.
- W2153683423 cites W2145336165 @default.
- W2153683423 cites W2146074997 @default.
- W2153683423 cites W2146396346 @default.
- W2153683423 cites W2146521959 @default.
- W2153683423 cites W2147783737 @default.
- W2153683423 cites W2155986110 @default.
- W2153683423 cites W2156145269 @default.
- W2153683423 cites W2158992333 @default.
- W2153683423 cites W2160456443 @default.
- W2153683423 cites W2160521790 @default.
- W2153683423 cites W2167062509 @default.
- W2153683423 cites W2167748132 @default.
- W2153683423 cites W2169330513 @default.
- W2153683423 doi "https://doi.org/10.1186/1471-2164-11-46" @default.
- W2153683423 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2822762" @default.
- W2153683423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20085629" @default.
- W2153683423 hasPublicationYear "2010" @default.
- W2153683423 type Work @default.
- W2153683423 sameAs 2153683423 @default.
- W2153683423 citedByCount "61" @default.
- W2153683423 countsByYear W21536834232012 @default.
- W2153683423 countsByYear W21536834232013 @default.
- W2153683423 countsByYear W21536834232014 @default.
- W2153683423 countsByYear W21536834232015 @default.
- W2153683423 countsByYear W21536834232016 @default.
- W2153683423 countsByYear W21536834232017 @default.
- W2153683423 countsByYear W21536834232018 @default.
- W2153683423 countsByYear W21536834232019 @default.
- W2153683423 countsByYear W21536834232021 @default.
- W2153683423 countsByYear W21536834232023 @default.
- W2153683423 crossrefType "journal-article" @default.
- W2153683423 hasAuthorship W2153683423A5020253377 @default.
- W2153683423 hasAuthorship W2153683423A5021701065 @default.
- W2153683423 hasAuthorship W2153683423A5027399260 @default.
- W2153683423 hasBestOaLocation W21536834231 @default.
- W2153683423 hasConcept C104317684 @default.
- W2153683423 hasConcept C132533263 @default.
- W2153683423 hasConcept C143121216 @default.
- W2153683423 hasConcept C15151743 @default.
- W2153683423 hasConcept C193252679 @default.
- W2153683423 hasConcept C203014093 @default.
- W2153683423 hasConcept C22744801 @default.
- W2153683423 hasConcept C2777385719 @default.
- W2153683423 hasConcept C2777695942 @default.
- W2153683423 hasConcept C2779676784 @default.