FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2153754889> ?p ?o ?g. }

• W2153754889 endingPage "335" @default.
• W2153754889 startingPage "325" @default.
• W2153754889 abstract "A 0.9-kb open reading frame encoding a unique 32-kDa protein was identified downstream of the recA gene of Porphyromonas gingivalis. Reverse transcription-PCR and Northern blot analysis showed that both the recA gene and this open reading frame are part of the same transcriptional unit. This cloned fragment was insertionally inactivated using the ermF-ermAM antibiotic resistance cassette to create a defective mutant by allelic exchange. When plated on Brucella blood agar, the mutant strain, designated P. gingivalis FLL92, was non-black pigmented and showed significant reduction in beta-hemolysis compared with the parent strain, P. gingivalis W83. Arginine- and lysine-specific cysteine protease activities, which were mostly soluble, were approximately 90% lower than that of the parent strain. Expression of the rgpA, rgpB, and kgp protease genes was the same in P. gingivalis FLL92 as in the wild-type strain. In contrast to the parent strain, P. gingivalis FLL92 showed increased autoaggregration in addition to a significant reduction in hemagglutinating and hemolysin activities. In in vivo experiments using a mouse model, P. gingivalis FLL92 was dramatically less virulent than the parent strain. A molecular survey of this mutant and the parent strain using all known P. gingivalis insertion sequence elements as probes suggested that no intragenomic changes due to the movement of these elements have occurred in P. gingivalis FLL92. Taken together, these results suggest that the recA downstream gene, designated vimA (virulence-modulating gene), plays an important role in virulence modulation in P. gingivalis W83, possibly representing a novel posttranscriptional or translational regulation of virulence factors in P. gingivalis." @default.
• W2153754889 created "2016-06-24" @default.
• W2153754889 creator A5005996766 @default.
• W2153754889 creator A5008659449 @default.
• W2153754889 creator A5011804479 @default.
• W2153754889 creator A5012547280 @default.
• W2153754889 creator A5017825677 @default.
• W2153754889 creator A5051149312 @default.
• W2153754889 date "2001-01-01" @default.
• W2153754889 modified "2023-10-18" @default.
• W2153754889 title "<i>vimA</i> Gene Downstream of <i>recA</i> Is Involved in Virulence Modulation in <i>Porphyromonas gingivalis</i> W83" @default.
• W2153754889 cites W1490293827 @default.
• W2153754889 cites W1514298611 @default.
• W2153754889 cites W1894773512 @default.
• W2153754889 cites W1918611745 @default.
• W2153754889 cites W1966169571 @default.
• W2153754889 cites W1972795930 @default.
• W2153754889 cites W1985917059 @default.
• W2153754889 cites W1991471459 @default.
• W2153754889 cites W1991825361 @default.
• W2153754889 cites W1993022619 @default.
• W2153754889 cites W1994384954 @default.
• W2153754889 cites W1999356499 @default.
• W2153754889 cites W2002385668 @default.
• W2153754889 cites W2002393139 @default.
• W2153754889 cites W2009310436 @default.
• W2153754889 cites W2010534284 @default.
• W2153754889 cites W2018289835 @default.
• W2153754889 cites W2019410656 @default.
• W2153754889 cites W2024465781 @default.
• W2153754889 cites W2028622989 @default.
• W2153754889 cites W2034378536 @default.
• W2153754889 cites W2038124483 @default.
• W2153754889 cites W2045053228 @default.
• W2153754889 cites W2048256811 @default.
• W2153754889 cites W2051588048 @default.
• W2153754889 cites W2053008139 @default.
• W2153754889 cites W2053757860 @default.
• W2153754889 cites W2054008251 @default.
• W2153754889 cites W2054826715 @default.
• W2153754889 cites W2055593270 @default.
• W2153754889 cites W2056066300 @default.
• W2153754889 cites W2056077453 @default.
• W2153754889 cites W2068518785 @default.
• W2153754889 cites W2071463327 @default.
• W2153754889 cites W2080094295 @default.
• W2153754889 cites W2084115323 @default.
• W2153754889 cites W2084544627 @default.
• W2153754889 cites W2087053665 @default.
• W2153754889 cites W2097474998 @default.
• W2153754889 cites W2100837269 @default.
• W2153754889 cites W2104164453 @default.
• W2153754889 cites W2107833602 @default.
• W2153754889 cites W2107836119 @default.
• W2153754889 cites W2113100474 @default.
• W2153754889 cites W2113552067 @default.
• W2153754889 cites W2114509351 @default.
• W2153754889 cites W2114554221 @default.
• W2153754889 cites W2122151275 @default.
• W2153754889 cites W2123449504 @default.
• W2153754889 cites W2125944744 @default.
• W2153754889 cites W2129310218 @default.
• W2153754889 cites W2133664523 @default.
• W2153754889 cites W2138270253 @default.
• W2153754889 cites W2143100467 @default.
• W2153754889 cites W2144849415 @default.
• W2153754889 cites W2148437996 @default.
• W2153754889 cites W2148582017 @default.
• W2153754889 cites W2149666020 @default.
• W2153754889 cites W2150260980 @default.
• W2153754889 cites W2151693749 @default.
• W2153754889 cites W2158725057 @default.
• W2153754889 cites W2163102348 @default.
• W2153754889 cites W2164313043 @default.
• W2153754889 cites W2343892901 @default.
• W2153754889 cites W2419171522 @default.
• W2153754889 doi "https://doi.org/10.1128/iai.69.1.325-335.2001" @default.
• W2153754889 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/97887" @default.
• W2153754889 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11119521" @default.
• W2153754889 hasPublicationYear "2001" @default.
• W2153754889 type Work @default.
• W2153754889 sameAs 2153754889 @default.
• W2153754889 citedByCount "57" @default.
• W2153754889 countsByYear W21537548892012 @default.
• W2153754889 countsByYear W21537548892013 @default.
• W2153754889 countsByYear W21537548892014 @default.
• W2153754889 countsByYear W21537548892015 @default.
• W2153754889 countsByYear W21537548892016 @default.
• W2153754889 countsByYear W21537548892017 @default.
• W2153754889 countsByYear W21537548892018 @default.
• W2153754889 countsByYear W21537548892019 @default.
• W2153754889 countsByYear W21537548892020 @default.
• W2153754889 countsByYear W21537548892021 @default.
• W2153754889 crossrefType "journal-article" @default.
• W2153754889 hasAuthorship W2153754889A5005996766 @default.
• W2153754889 hasAuthorship W2153754889A5008659449 @default.
• W2153754889 hasAuthorship W2153754889A5011804479 @default.
• W2153754889 hasAuthorship W2153754889A5012547280 @default.

• next