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- W2153869337 abstract "The first complete genome sequence for a myoviridal bacteriophage, PhiEa21-4, infecting Erwinia amylovora, Erwinia pyrifoliae, and Pantoea agglomerans strains has been determined. The unique sequence of this terminally redundant, circularly permuted genome is 84,576 bp. The PhiEa21-4 genome has a GC content of 43.8% and contains 117 putative protein-coding genes and 26 tRNA genes. PhiEa21-4 is the first phage in which a precisely conserved rho-independent terminator has been found dispersed throughout the genome, with 24 copies in all. Also notable in the PhiEa21-4 genome are the presence of tRNAs with six- and nine-base anticodon loops, the absence of a small packaging terminase subunit, and the presence of nadV, a principle component of the NAD(+) salvage pathway, which has been found in only a few phage genomes to date. PhiEa21-4 is the first reported Felix O1-like phage genome; 56% of the predicted PhiEa21-4 proteins share homology with those of the Salmonella phage. Apart from this similarity to Felix O1, the PhiEa21-4 genome appears to be substantially different, both globally and locally, from previously reported sequences. A total of 43 of the 117 genes are unique to PhiEa21-4, and 32 of the Felix O1-like genes do not appear in any phage genome sequences other than PhiEa21-4 and Felix O1. N-terminal sequencing and matrix-assisted laser desorption ionization-time of flight analysis resulted in the identification of five PhiEa21-4 genes coding for virion structural proteins, including the major capsid protein." @default.
- W2153869337 created "2016-06-24" @default.
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- W2153869337 creator A5072146758 @default.
- W2153869337 date "2009-04-01" @default.
- W2153869337 modified "2023-09-30" @default.
- W2153869337 title "Complete Genome of the Broad-Host-Range <i>Erwinia amylovora</i> Phage ΦEa21-4 and Its Relationship to <i>Salmonella</i> Phage Felix O1" @default.
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- W2153869337 doi "https://doi.org/10.1128/aem.02352-08" @default.
- W2153869337 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2663227" @default.
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