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• W2153959639 endingPage "1067" @default.
• W2153959639 startingPage "1059" @default.
• W2153959639 abstract "SUMMARY V-ATPases are complex proteins consisting of a peripheral, ATP-hydrolysing V1 complex and a membrane-bound H+-translocating Vo complex. The plasma membrane V-ATPase from the tobacco hornworm(Manduca sexta) midgut is made up of eight different V1and four different Vo subunits. During starvation and moulting,V-ATPase activity decreases as a result of the dissociation of the V1 complex from the Vo complex. To determine whether subunit biosynthesis is reduced during periods of enzyme inactivity, we measured the transcript levels and transcriptional activities of V-ATPase genes. Northern blots revealed the downregulation of almost all V-ATPase transcripts during starvation. During moulting, transcript levels of the three V-ATPase genes examined, mvB, mvG and mvd, also decreased,and this decrease was negatively correlated with the titre of 20-hydroxyecdysone (20-HE) and positively correlated with the titre of juvenile hormone (JH). To test the biological significance of these correlations, we injected both hormones into feeding larvae and measured transcript levels several hours later. A short-term increase and a long-term decrease in levels of mRNA were observed after 20-HE injection, whereas JH injection had no significant effect. Immunohistochemical studies of the midgut epithelium revealed that 20-HE injection led to changes in goblet cell morphology and in the subcellular distribution of the V1 complex comparable with the situation during the moult and during starvation. Reporter gene assays in Sf21 cells using mvB, mvG and mvdpromoters to initiate transcription of firefly luciferase led, after incubation of the cells with 20-HE, to results comparable with those obtained in the injection experiments. These findings suggest that putative ecdysone-responsive elements are present in all three promoters. Taken together, our results suggest that the expression of V-ATPase genes is controlled in a coordinated manner by ecdysteroids." @default.
• W2153959639 created "2016-06-24" @default.
• W2153959639 creator A5003406909 @default.
• W2153959639 creator A5066946579 @default.
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• W2153959639 date "2002-04-15" @default.
• W2153959639 modified "2023-10-16" @default.
• W2153959639 title "Expression of<i>Manduca sexta</i>V-ATPase genes<i>mvB, mvG</i>and<i>mvd</i>is regulated by ecdysteroids" @default.
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• W2153959639 doi "https://doi.org/10.1242/jeb.205.8.1059" @default.
• W2153959639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11919265" @default.
• W2153959639 hasPublicationYear "2002" @default.
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