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- W2154007113 abstract "Abstract Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD + ‐dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli . In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD + as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature‐sensitive mutation in ligA . Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD + ‐dependent DNA ligases. Proteins 2003;51:321–326. © 2003 Wiley‐Liss, Inc." @default.
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- W2154007113 date "2003-04-08" @default.
- W2154007113 modified "2023-10-16" @default.
- W2154007113 title "NAD-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor" @default.
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- W2154007113 doi "https://doi.org/10.1002/prot.10361" @default.
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