FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154151010> ?p ?o ?g. }

• W2154151010 abstract "Deletion mutagenesis was used to define the core region of the catalytic subunit of rabbit muscle protein phosphatase-1. Deletions in the N terminus were found to lead to loss of expression. Deletions of up to 33 residues from the C-terminal region were tolerated, and the truncated enzymes were fully active. Deletion of an additional 21 residues led to loss of expression. Mutants which had had 33 and 25 residues deleted maintained specific activities that were comparable to those of the wild type enzyme. The response of these two deletion mutants to okadaic acid, microcystin, and inhibitor-2 was determined. Only slightly lower IC50 values were observed in all cases, showing that the C terminus itself does not play a major role in the binding of these inhibitors. The deletion mutants formed stable complexes with inhibitor-2 as shown by gel filtration. These studies provide unambiguous evidence that the extreme C-terminal region of protein phosphatase-1 is not directly involved in catalytic function or in the binding of inhibitor-2, microcystin, or okadaic acid, and they also establish that the first approximately 300 residues of the sequence constitute a sufficient core for protein phosphatase-1 catalytic functions." @default.
• W2154151010 created "2016-06-24" @default.
• W2154151010 creator A5013290708 @default.
• W2154151010 creator A5033822905 @default.
• W2154151010 creator A5044741719 @default.
• W2154151010 creator A5060646510 @default.
• W2154151010 date "1994-05-01" @default.
• W2154151010 modified "2023-09-29" @default.
• W2154151010 title "Characterization of deletion mutants of the catalytic subunit of protein phosphatase-1." @default.
• W2154151010 cites W1488248876 @default.
• W2154151010 cites W1496832150 @default.
• W2154151010 cites W1513576040 @default.
• W2154151010 cites W1526307219 @default.
• W2154151010 cites W1528249941 @default.
• W2154151010 cites W1561391742 @default.
• W2154151010 cites W1581498719 @default.
• W2154151010 cites W1591116477 @default.
• W2154151010 cites W1592385070 @default.
• W2154151010 cites W1749352888 @default.
• W2154151010 cites W1965727075 @default.
• W2154151010 cites W1972663144 @default.
• W2154151010 cites W1974657301 @default.
• W2154151010 cites W1975123460 @default.
• W2154151010 cites W1975304761 @default.
• W2154151010 cites W1978967216 @default.
• W2154151010 cites W1980051653 @default.
• W2154151010 cites W1983620691 @default.
• W2154151010 cites W1986306967 @default.
• W2154151010 cites W1991359253 @default.
• W2154151010 cites W1998366755 @default.
• W2154151010 cites W1998410161 @default.
• W2154151010 cites W2003964594 @default.
• W2154151010 cites W2014285665 @default.
• W2154151010 cites W2039918366 @default.
• W2154151010 cites W2045434651 @default.
• W2154151010 cites W2063127137 @default.
• W2154151010 cites W2064248967 @default.
• W2154151010 cites W2067316290 @default.
• W2154151010 cites W2078160615 @default.
• W2154151010 cites W2079067261 @default.
• W2154151010 cites W2079906375 @default.
• W2154151010 cites W2082509930 @default.
• W2154151010 cites W2089969587 @default.
• W2154151010 cites W2100837269 @default.
• W2154151010 cites W2106104578 @default.
• W2154151010 cites W2178456244 @default.
• W2154151010 cites W4211245717 @default.
• W2154151010 cites W4293247451 @default.
• W2154151010 doi "https://doi.org/10.1016/s0021-9258(17)36713-3" @default.
• W2154151010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8188653" @default.
• W2154151010 hasPublicationYear "1994" @default.
• W2154151010 type Work @default.
• W2154151010 sameAs 2154151010 @default.
• W2154151010 citedByCount "16" @default.
• W2154151010 countsByYear W21541510102012 @default.
• W2154151010 crossrefType "journal-article" @default.
• W2154151010 hasAuthorship W2154151010A5013290708 @default.
• W2154151010 hasAuthorship W2154151010A5033822905 @default.
• W2154151010 hasAuthorship W2154151010A5044741719 @default.
• W2154151010 hasAuthorship W2154151010A5060646510 @default.
• W2154151010 hasBestOaLocation W21541510101 @default.
• W2154151010 hasConcept C103408237 @default.
• W2154151010 hasConcept C104292427 @default.
• W2154151010 hasConcept C104317684 @default.
• W2154151010 hasConcept C143065580 @default.
• W2154151010 hasConcept C153911025 @default.
• W2154151010 hasConcept C16318435 @default.
• W2154151010 hasConcept C178666793 @default.
• W2154151010 hasConcept C181199279 @default.
• W2154151010 hasConcept C185592680 @default.
• W2154151010 hasConcept C2776953658 @default.
• W2154151010 hasConcept C2779408958 @default.
• W2154151010 hasConcept C389152 @default.
• W2154151010 hasConcept C55493867 @default.
• W2154151010 hasConcept C86803240 @default.
• W2154151010 hasConcept C97920573 @default.
• W2154151010 hasConceptScore W2154151010C103408237 @default.
• W2154151010 hasConceptScore W2154151010C104292427 @default.
• W2154151010 hasConceptScore W2154151010C104317684 @default.
• W2154151010 hasConceptScore W2154151010C143065580 @default.
• W2154151010 hasConceptScore W2154151010C153911025 @default.
• W2154151010 hasConceptScore W2154151010C16318435 @default.
• W2154151010 hasConceptScore W2154151010C178666793 @default.
• W2154151010 hasConceptScore W2154151010C181199279 @default.
• W2154151010 hasConceptScore W2154151010C185592680 @default.
• W2154151010 hasConceptScore W2154151010C2776953658 @default.
• W2154151010 hasConceptScore W2154151010C2779408958 @default.
• W2154151010 hasConceptScore W2154151010C389152 @default.
• W2154151010 hasConceptScore W2154151010C55493867 @default.
• W2154151010 hasConceptScore W2154151010C86803240 @default.
• W2154151010 hasConceptScore W2154151010C97920573 @default.
• W2154151010 hasLocation W21541510101 @default.
• W2154151010 hasOpenAccess W2154151010 @default.
• W2154151010 hasPrimaryLocation W21541510101 @default.
• W2154151010 hasRelatedWork W1506014073 @default.
• W2154151010 hasRelatedWork W1563232494 @default.
• W2154151010 hasRelatedWork W1596154245 @default.
• W2154151010 hasRelatedWork W1892570697 @default.
• W2154151010 hasRelatedWork W1965837065 @default.
• W2154151010 hasRelatedWork W1969173610 @default.

• next