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• W2154222012 abstract "ABSTRACT Volatile sulfur compounds, including hydrogen sulfide (H 2 S), have been implicated in the development of periodontal disease. Glutathione is an important thiol source for H 2 S production in periodontal pockets. Our recent studies have delineated a pathway of glutathione metabolism in Treponema denticola that releases H 2 S. In this pathway, γ-glutamyltransferase (GGT) has been proposed to catalyze the first step of glutathione degradation. We have cloned the gene of GGT from T. denticola , which contains an open reading frame of 726 bp encoding a protein of 241 amino acids. Transformation of this gene into Escherichia coli led to the expression of a recombinant protein. After purification by chromatography, the recombinant protein showed enzymatic activity typical of GGT, catalyzing the degradation of Na-γ-glutamyl-4-nitroaniline (GNA) and the hydrolysis of glutathione, releasing glutamic acid or glutamine and cysteinylglycine. l -Cysteine is not a substrate of GGT. Importantly, GNA, when added to T. denticola , was able to compete with glutathione and inhibit the production of H 2 S, ammonia, and pyruvate. This was accompanied by the suppression of hemoxidative and hemolytic activities of the bacteria. Purified GGT was inactivated by TLCK ( N α- p -tosyl- l -lysine chloromethyl ketone) and proteinase K treatment. However, higher enzymatic activity was demonstrated in the presence of 2-mercaptoethanol and dithiothreitol. Our further experiments showed that the addition of recombinant GGT to Porphyromonas gingivalis , a bacterium without significant glutathione-metabolizing capacity, drastically increased the utilization of glutathione by the bacterium, producing H 2 S, ammonia, and pyruvate. This was again accompanied by enhanced bacterial hemoxidative and hemolytic activities. Together, the results suggest an important role for GGT in glutathione metabolism in oral bacteria." @default.
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• W2154222012 date "2003-01-01" @default.
• W2154222012 modified "2023-09-23" @default.
• W2154222012 title "Role for Recombinant γ-Glutamyltransferase from Treponema denticola in Glutathione Metabolism" @default.
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• W2154222012 doi "https://doi.org/10.1128/iai.71.1.335-342.2003" @default.
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