Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154223003> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2154223003 endingPage "4056" @default.
- W2154223003 startingPage "4051" @default.
- W2154223003 abstract "Haloalcohol dehalogenases are bacterial enzymes that cleave the carbon-halogen bond in short aliphatic vicinal haloalcohols, like 1-chloro-2,3-propanediol, some of which are recalcitrant environmental pollutants. They use a conserved Ser-Tyr-Arg catalytic triad to deprotonate the haloalcohol oxygen, which attacks the halogen-bearing carbon atom, producing an epoxide and a halide ion. Here, we present the X-ray structure of the haloalcohol dehalogenase HheA(AD2) from Arthrobacter sp. strain AD2 at 2.0-A resolution. Comparison with the previously reported structure of the 34% identical enantioselective haloalcohol dehalogenase HheC from Agrobacterium radiobacter AD1 shows that HheA(AD2) has a similar quaternary and tertiary structure but a much more open substrate-binding pocket. Docking experiments reveal that HheA(AD2) can bind both enantiomers of the haloalcohol substrate 1-p-nitrophenyl-2-chloroethanol in a productive way, which explains the low enantiopreference of HheA(AD2). Other differences are found in the halide-binding site, where the side chain amino group of Asn182 is in a position to stabilize the halogen atom or halide ion in HheA(AD2), in contrast to HheC, where a water molecule has taken over this role. These results broaden the insight into the structural determinants that govern reactivity and selectivity in the haloalcohol dehalogenase family." @default.
- W2154223003 created "2016-06-24" @default.
- W2154223003 creator A5040913948 @default.
- W2154223003 creator A5060858412 @default.
- W2154223003 creator A5066632471 @default.
- W2154223003 creator A5076930474 @default.
- W2154223003 creator A5084133867 @default.
- W2154223003 date "2006-06-01" @default.
- W2154223003 modified "2023-09-26" @default.
- W2154223003 title "The X-Ray Structure of the Haloalcohol Dehalogenase HheA from <i>Arthrobacter</i> sp. Strain AD2: Insight into Enantioselectivity and Halide Binding in the Haloalcohol Dehalogenase Family" @default.
- W2154223003 cites W1539796472 @default.
- W2154223003 cites W1563191931 @default.
- W2154223003 cites W1982608575 @default.
- W2154223003 cites W1986191025 @default.
- W2154223003 cites W1988530421 @default.
- W2154223003 cites W1993404146 @default.
- W2154223003 cites W2001641653 @default.
- W2154223003 cites W2039369222 @default.
- W2154223003 cites W2059890422 @default.
- W2154223003 cites W2105055623 @default.
- W2154223003 cites W2106154707 @default.
- W2154223003 cites W2120434310 @default.
- W2154223003 cites W2125162418 @default.
- W2154223003 cites W2128906655 @default.
- W2154223003 cites W2129300174 @default.
- W2154223003 cites W2134160548 @default.
- W2154223003 cites W2135839939 @default.
- W2154223003 cites W2144689014 @default.
- W2154223003 cites W2151449293 @default.
- W2154223003 cites W2154689668 @default.
- W2154223003 cites W4252114087 @default.
- W2154223003 doi "https://doi.org/10.1128/jb.01866-05" @default.
- W2154223003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1482898" @default.
- W2154223003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16707696" @default.
- W2154223003 hasPublicationYear "2006" @default.
- W2154223003 type Work @default.
- W2154223003 sameAs 2154223003 @default.
- W2154223003 citedByCount "32" @default.
- W2154223003 countsByYear W21542230032012 @default.
- W2154223003 countsByYear W21542230032013 @default.
- W2154223003 countsByYear W21542230032014 @default.
- W2154223003 countsByYear W21542230032015 @default.
- W2154223003 countsByYear W21542230032016 @default.
- W2154223003 countsByYear W21542230032019 @default.
- W2154223003 countsByYear W21542230032020 @default.
- W2154223003 countsByYear W21542230032021 @default.
- W2154223003 countsByYear W21542230032022 @default.
- W2154223003 crossrefType "journal-article" @default.
- W2154223003 hasAuthorship W2154223003A5040913948 @default.
- W2154223003 hasAuthorship W2154223003A5060858412 @default.
- W2154223003 hasAuthorship W2154223003A5066632471 @default.
- W2154223003 hasAuthorship W2154223003A5076930474 @default.
- W2154223003 hasAuthorship W2154223003A5084133867 @default.
- W2154223003 hasBestOaLocation W21542230032 @default.
- W2154223003 hasConcept C145770059 @default.
- W2154223003 hasConcept C181199279 @default.
- W2154223003 hasConcept C185592680 @default.
- W2154223003 hasConcept C2777485300 @default.
- W2154223003 hasConcept C41183919 @default.
- W2154223003 hasConcept C55493867 @default.
- W2154223003 hasConcept C71240020 @default.
- W2154223003 hasConcept C86803240 @default.
- W2154223003 hasConceptScore W2154223003C145770059 @default.
- W2154223003 hasConceptScore W2154223003C181199279 @default.
- W2154223003 hasConceptScore W2154223003C185592680 @default.
- W2154223003 hasConceptScore W2154223003C2777485300 @default.
- W2154223003 hasConceptScore W2154223003C41183919 @default.
- W2154223003 hasConceptScore W2154223003C55493867 @default.
- W2154223003 hasConceptScore W2154223003C71240020 @default.
- W2154223003 hasConceptScore W2154223003C86803240 @default.
- W2154223003 hasIssue "11" @default.
- W2154223003 hasLocation W21542230031 @default.
- W2154223003 hasLocation W21542230032 @default.
- W2154223003 hasLocation W21542230033 @default.
- W2154223003 hasLocation W21542230034 @default.
- W2154223003 hasLocation W21542230035 @default.
- W2154223003 hasLocation W21542230036 @default.
- W2154223003 hasOpenAccess W2154223003 @default.
- W2154223003 hasPrimaryLocation W21542230031 @default.
- W2154223003 hasRelatedWork W2006627653 @default.
- W2154223003 hasRelatedWork W2014252525 @default.
- W2154223003 hasRelatedWork W2022952366 @default.
- W2154223003 hasRelatedWork W2037631993 @default.
- W2154223003 hasRelatedWork W2071763012 @default.
- W2154223003 hasRelatedWork W2082917051 @default.
- W2154223003 hasRelatedWork W2087134015 @default.
- W2154223003 hasRelatedWork W2093753737 @default.
- W2154223003 hasRelatedWork W2155257397 @default.
- W2154223003 hasRelatedWork W2520015457 @default.
- W2154223003 hasVolume "188" @default.
- W2154223003 isParatext "false" @default.
- W2154223003 isRetracted "false" @default.
- W2154223003 magId "2154223003" @default.
- W2154223003 workType "article" @default.