FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154264902> ?p ?o ?g. }

• W2154264902 endingPage "426" @default.
• W2154264902 startingPage "415" @default.
• W2154264902 abstract "A glucoamylase::green fluorescent protein fusion (GLA::sGFP) was constructed which allows the green fluorescent protein to be used as an in vivo reporter of protein secretion in Aspergillus niger. Two secretory fusions were designed for secretion of GLA::sGFP which employed slightly different lengths of the glucoamylase protein (GLA499 and GLA514). Expression of GLA::sGFP revealed that fluorescence was localized in the hyphal cell walls and septa, and that fluorescence was most intense at hyphal apices. Extracellular GLA::sGFP was detectable by Western blotting only in the supernatant of young cultures grown in soya milk medium. In older cultures, acidification of the medium and induction of proteases were probably responsible for the loss of extracellular and cell wall fluorescence and the inability to detect GLA::sGFP by Western analysis. A strain containing the GLA::sGFP construct was subjected to UV mutagenesis and survivors screened for mutations in the general secretory pathway. Three mutants were isolated that were unable to form a halo on either starch or gelatin medium. All three mutants grew poorly compared to the parental strain. Fluorescence microscopy revealed that for two of the mutants, GLA::sGFP accumulated intracellularly with no evidence of wall fluorescence, whereas for the third mutant, wall fluorescence was observed with no evidence of intracellular accumulation. These results indicate that the GLA::sGFP fusion constructs can be used as convenient fluorescent markers to study the dynamics of protein secretion in vivo and as a tool in the isolation of mutants in the general secretory pathway." @default.
• W2154264902 created "2016-06-24" @default.
• W2154264902 creator A5032557742 @default.
• W2154264902 creator A5033651373 @default.
• W2154264902 creator A5034104737 @default.
• W2154264902 creator A5034823297 @default.
• W2154264902 creator A5039181800 @default.
• W2154264902 creator A5041257531 @default.
• W2154264902 creator A5045841552 @default.
• W2154264902 creator A5058074531 @default.
• W2154264902 creator A5065088708 @default.
• W2154264902 creator A5065740696 @default.
• W2154264902 creator A5067592841 @default.
• W2154264902 creator A5072964205 @default.
• W2154264902 date "2000-02-01" @default.
• W2154264902 modified "2023-09-26" @default.
• W2154264902 title "Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger" @default.
• W2154264902 cites W11988849 @default.
• W2154264902 cites W1520282413 @default.
• W2154264902 cites W1555329158 @default.
• W2154264902 cites W1908376554 @default.
• W2154264902 cites W1965267392 @default.
• W2154264902 cites W1967171950 @default.
• W2154264902 cites W1975044278 @default.
• W2154264902 cites W1978208012 @default.
• W2154264902 cites W1980073547 @default.
• W2154264902 cites W1983349127 @default.
• W2154264902 cites W1995549160 @default.
• W2154264902 cites W2006685379 @default.
• W2154264902 cites W2007405937 @default.
• W2154264902 cites W2019777184 @default.
• W2154264902 cites W2023066425 @default.
• W2154264902 cites W2023767092 @default.
• W2154264902 cites W2025943607 @default.
• W2154264902 cites W2026137685 @default.
• W2154264902 cites W2026601439 @default.
• W2154264902 cites W2034194918 @default.
• W2154264902 cites W2038756774 @default.
• W2154264902 cites W2039722369 @default.
• W2154264902 cites W2042022922 @default.
• W2154264902 cites W2050828271 @default.
• W2154264902 cites W2053248821 @default.
• W2154264902 cites W2058519324 @default.
• W2154264902 cites W2059909013 @default.
• W2154264902 cites W2063450941 @default.
• W2154264902 cites W2082205744 @default.
• W2154264902 cites W2089024466 @default.
• W2154264902 cites W2098998842 @default.
• W2154264902 cites W2101108802 @default.
• W2154264902 cites W2103758980 @default.
• W2154264902 cites W2116661995 @default.
• W2154264902 cites W2128625412 @default.
• W2154264902 cites W2130652548 @default.
• W2154264902 cites W2131974188 @default.
• W2154264902 cites W2140647347 @default.
• W2154264902 cites W2141590023 @default.
• W2154264902 cites W2145351936 @default.
• W2154264902 cites W2170236982 @default.
• W2154264902 cites W2172088966 @default.
• W2154264902 cites W2325947050 @default.
• W2154264902 cites W4236962085 @default.
• W2154264902 doi "https://doi.org/10.1099/00221287-146-2-415" @default.
• W2154264902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10708380" @default.
• W2154264902 hasPublicationYear "2000" @default.
• W2154264902 type Work @default.
• W2154264902 sameAs 2154264902 @default.
• W2154264902 citedByCount "110" @default.
• W2154264902 countsByYear W21542649022012 @default.
• W2154264902 countsByYear W21542649022013 @default.
• W2154264902 countsByYear W21542649022014 @default.
• W2154264902 countsByYear W21542649022015 @default.
• W2154264902 countsByYear W21542649022016 @default.
• W2154264902 countsByYear W21542649022017 @default.
• W2154264902 countsByYear W21542649022018 @default.
• W2154264902 countsByYear W21542649022019 @default.
• W2154264902 countsByYear W21542649022020 @default.
• W2154264902 countsByYear W21542649022021 @default.
• W2154264902 countsByYear W21542649022022 @default.
• W2154264902 crossrefType "journal-article" @default.
• W2154264902 hasAuthorship W2154264902A5032557742 @default.
• W2154264902 hasAuthorship W2154264902A5033651373 @default.
• W2154264902 hasAuthorship W2154264902A5034104737 @default.
• W2154264902 hasAuthorship W2154264902A5034823297 @default.
• W2154264902 hasAuthorship W2154264902A5039181800 @default.
• W2154264902 hasAuthorship W2154264902A5041257531 @default.
• W2154264902 hasAuthorship W2154264902A5045841552 @default.
• W2154264902 hasAuthorship W2154264902A5058074531 @default.
• W2154264902 hasAuthorship W2154264902A5065088708 @default.
• W2154264902 hasAuthorship W2154264902A5065740696 @default.
• W2154264902 hasAuthorship W2154264902A5067592841 @default.
• W2154264902 hasAuthorship W2154264902A5072964205 @default.
• W2154264902 hasBestOaLocation W21542649021 @default.
• W2154264902 hasConcept C104317684 @default.
• W2154264902 hasConcept C121332964 @default.
• W2154264902 hasConcept C123894998 @default.
• W2154264902 hasConcept C142613039 @default.
• W2154264902 hasConcept C143065580 @default.
• W2154264902 hasConcept C153911025 @default.

• next