FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2154271698> ?p ?o ?g. }

• W2154271698 endingPage "34" @default.
• W2154271698 startingPage "28" @default.
• W2154271698 abstract "White spot syndrome virus (WSSV) continues to be the most pathogenic among the penaeid shrimp viruses. In this study, WSSV DNA was detected in pond soil samples using a 2-step nested PCR. Primers described previously were used for the first round of amplification and based on the sequenced amplicon, an inner primer was designed for the 2nd round of amplification. Using plasmid DNA (pET 100) containing the 211 bp target WSSV sequence, analytical sensitivity showed that the 2-step nested PCR protocol was able to detect down to 0.015 fg of the plasmid DNA, or approximately 2 copies of the target DNA sequence. Persistence of WSSV DNA in pond soil samples after various time intervals was determined. WSSV-specific PCR product (161 bp) was still present in the soil samples even after 10 months of storage. The effect of soil heat treatment on the WSSV DNA was also examined. Soils were subjected to 25, 37, 50 and 70 degrees C for 1, 3 and 5 days. The results showed that PCR amplifiable WSSV DNA was still present even after 5 days at 70 degrees C. To our knowledge, this is the first report on the detection of WSSV DNA in soil samples. Based on these findings, it is concluded that the persistence of viral DNA in soil habitats may be an important aspect of WSSV ecology and may have an implication for viral transmissibility." @default.
• W2154271698 created "2016-06-24" @default.
• W2154271698 creator A5042509544 @default.
• W2154271698 creator A5072804809 @default.
• W2154271698 creator A5083225988 @default.
• W2154271698 date "2008-04-01" @default.
• W2154271698 modified "2023-09-30" @default.
• W2154271698 title "Detection of White spot syndrome virus DNA in pond soil using a 2-step nested PCR" @default.
• W2154271698 cites W146138009 @default.
• W2154271698 cites W1873633121 @default.
• W2154271698 cites W1969126148 @default.
• W2154271698 cites W1973970366 @default.
• W2154271698 cites W1980042844 @default.
• W2154271698 cites W1990700109 @default.
• W2154271698 cites W1998989192 @default.
• W2154271698 cites W2014672236 @default.
• W2154271698 cites W2050629636 @default.
• W2154271698 cites W2055241965 @default.
• W2154271698 cites W2059367464 @default.
• W2154271698 cites W2062320783 @default.
• W2154271698 cites W2062389463 @default.
• W2154271698 cites W2065982708 @default.
• W2154271698 cites W2071850340 @default.
• W2154271698 cites W2072535601 @default.
• W2154271698 cites W2072954203 @default.
• W2154271698 cites W2075667802 @default.
• W2154271698 cites W2077493149 @default.
• W2154271698 cites W2082730827 @default.
• W2154271698 cites W2129701786 @default.
• W2154271698 cites W2138270253 @default.
• W2154271698 cites W2143855217 @default.
• W2154271698 cites W2145814889 @default.
• W2154271698 cites W2149194655 @default.
• W2154271698 cites W2161784652 @default.
• W2154271698 cites W2329660842 @default.
• W2154271698 doi "https://doi.org/10.1016/j.jviromet.2008.01.013" @default.
• W2154271698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18313145" @default.
• W2154271698 hasPublicationYear "2008" @default.
• W2154271698 type Work @default.
• W2154271698 sameAs 2154271698 @default.
• W2154271698 citedByCount "27" @default.
• W2154271698 countsByYear W21542716982012 @default.
• W2154271698 countsByYear W21542716982013 @default.
• W2154271698 countsByYear W21542716982014 @default.
• W2154271698 countsByYear W21542716982015 @default.
• W2154271698 countsByYear W21542716982017 @default.
• W2154271698 countsByYear W21542716982019 @default.
• W2154271698 countsByYear W21542716982020 @default.
• W2154271698 countsByYear W21542716982022 @default.
• W2154271698 countsByYear W21542716982023 @default.
• W2154271698 crossrefType "journal-article" @default.
• W2154271698 hasAuthorship W2154271698A5042509544 @default.
• W2154271698 hasAuthorship W2154271698A5072804809 @default.
• W2154271698 hasAuthorship W2154271698A5083225988 @default.
• W2154271698 hasConcept C104317684 @default.
• W2154271698 hasConcept C153911025 @default.
• W2154271698 hasConcept C157431215 @default.
• W2154271698 hasConcept C159047783 @default.
• W2154271698 hasConcept C178790620 @default.
• W2154271698 hasConcept C185592680 @default.
• W2154271698 hasConcept C18903297 @default.
• W2154271698 hasConcept C22744801 @default.
• W2154271698 hasConcept C2522874641 @default.
• W2154271698 hasConcept C2776841996 @default.
• W2154271698 hasConcept C2777563447 @default.
• W2154271698 hasConcept C2779771762 @default.
• W2154271698 hasConcept C49105822 @default.
• W2154271698 hasConcept C52001828 @default.
• W2154271698 hasConcept C54355233 @default.
• W2154271698 hasConcept C552990157 @default.
• W2154271698 hasConcept C78063203 @default.
• W2154271698 hasConcept C8185291 @default.
• W2154271698 hasConcept C86803240 @default.
• W2154271698 hasConcept C90583042 @default.
• W2154271698 hasConceptScore W2154271698C104317684 @default.
• W2154271698 hasConceptScore W2154271698C153911025 @default.
• W2154271698 hasConceptScore W2154271698C157431215 @default.
• W2154271698 hasConceptScore W2154271698C159047783 @default.
• W2154271698 hasConceptScore W2154271698C178790620 @default.
• W2154271698 hasConceptScore W2154271698C185592680 @default.
• W2154271698 hasConceptScore W2154271698C18903297 @default.
• W2154271698 hasConceptScore W2154271698C22744801 @default.
• W2154271698 hasConceptScore W2154271698C2522874641 @default.
• W2154271698 hasConceptScore W2154271698C2776841996 @default.
• W2154271698 hasConceptScore W2154271698C2777563447 @default.
• W2154271698 hasConceptScore W2154271698C2779771762 @default.
• W2154271698 hasConceptScore W2154271698C49105822 @default.
• W2154271698 hasConceptScore W2154271698C52001828 @default.
• W2154271698 hasConceptScore W2154271698C54355233 @default.
• W2154271698 hasConceptScore W2154271698C552990157 @default.
• W2154271698 hasConceptScore W2154271698C78063203 @default.
• W2154271698 hasConceptScore W2154271698C8185291 @default.
• W2154271698 hasConceptScore W2154271698C86803240 @default.
• W2154271698 hasConceptScore W2154271698C90583042 @default.
• W2154271698 hasIssue "1" @default.
• W2154271698 hasLocation W21542716981 @default.
• W2154271698 hasLocation W21542716982 @default.
• W2154271698 hasOpenAccess W2154271698 @default.

• next